Quantitative analysis of calcium spikes in noisy fluorescent background.

Intracellular calcium signals are studied by laser-scanning confocal fluorescence microscopy. The required spatio-temporal resolution makes description of calcium signals difficult because of the low signal-to-noise ratio. We designed a new procedure of calcium spike analysis based on their fitting...

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Autores principales:	Radoslav Janicek, Matej Hotka, Alexandra Zahradníková, Ivan Zahradník
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2da4f72ae9844ef89bebe140b19aa16
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spelling	oai:doaj.org-article:e2da4f72ae9844ef89bebe140b19aa162021-11-18T07:43:39ZQuantitative analysis of calcium spikes in noisy fluorescent background.1932-620310.1371/journal.pone.0064394https://doaj.org/article/e2da4f72ae9844ef89bebe140b19aa162013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23741324/?tool=EBIhttps://doaj.org/toc/1932-6203Intracellular calcium signals are studied by laser-scanning confocal fluorescence microscopy. The required spatio-temporal resolution makes description of calcium signals difficult because of the low signal-to-noise ratio. We designed a new procedure of calcium spike analysis based on their fitting with a model. The accuracy and precision of calcium spike description were tested on synthetic datasets generated either with randomly varied spike parameters and Gaussian noise of constant amplitude, or with constant spike parameters and Gaussian noise of various amplitudes. Statistical analysis was used to evaluate the performance of spike fitting algorithms. The procedure was optimized for reliable estimation of calcium spike parameters and for dismissal of false events. A new algorithm was introduced that corrects the acquisition time of pixels in line-scan images that is in error due to sequential acquisition of individual pixels along the space coordinate. New software was developed in Matlab and provided for general use. It allows interactive dissection of temporal profiles of calcium spikes from x-t images, their fitting with predefined function(s) and acceptance of results on statistical grounds, thus allowing efficient analysis and reliable description of calcium signaling in cardiac myocytes down to the in situ function of ryanodine receptors.Radoslav JanicekMatej HotkaAlexandra ZahradníkováAlexandra ZahradníkováIvan ZahradníkPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64394 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Radoslav Janicek Matej Hotka Alexandra Zahradníková Alexandra Zahradníková Ivan Zahradník Quantitative analysis of calcium spikes in noisy fluorescent background.
description	Intracellular calcium signals are studied by laser-scanning confocal fluorescence microscopy. The required spatio-temporal resolution makes description of calcium signals difficult because of the low signal-to-noise ratio. We designed a new procedure of calcium spike analysis based on their fitting with a model. The accuracy and precision of calcium spike description were tested on synthetic datasets generated either with randomly varied spike parameters and Gaussian noise of constant amplitude, or with constant spike parameters and Gaussian noise of various amplitudes. Statistical analysis was used to evaluate the performance of spike fitting algorithms. The procedure was optimized for reliable estimation of calcium spike parameters and for dismissal of false events. A new algorithm was introduced that corrects the acquisition time of pixels in line-scan images that is in error due to sequential acquisition of individual pixels along the space coordinate. New software was developed in Matlab and provided for general use. It allows interactive dissection of temporal profiles of calcium spikes from x-t images, their fitting with predefined function(s) and acceptance of results on statistical grounds, thus allowing efficient analysis and reliable description of calcium signaling in cardiac myocytes down to the in situ function of ryanodine receptors.
format	article
author	Radoslav Janicek Matej Hotka Alexandra Zahradníková Alexandra Zahradníková Ivan Zahradník
author_facet	Radoslav Janicek Matej Hotka Alexandra Zahradníková Alexandra Zahradníková Ivan Zahradník
author_sort	Radoslav Janicek
title	Quantitative analysis of calcium spikes in noisy fluorescent background.
title_short	Quantitative analysis of calcium spikes in noisy fluorescent background.
title_full	Quantitative analysis of calcium spikes in noisy fluorescent background.
title_fullStr	Quantitative analysis of calcium spikes in noisy fluorescent background.
title_full_unstemmed	Quantitative analysis of calcium spikes in noisy fluorescent background.
title_sort	quantitative analysis of calcium spikes in noisy fluorescent background.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e2da4f72ae9844ef89bebe140b19aa16
work_keys_str_mv	AT radoslavjanicek quantitativeanalysisofcalciumspikesinnoisyfluorescentbackground AT matejhotka quantitativeanalysisofcalciumspikesinnoisyfluorescentbackground AT alexandrazahradnikova quantitativeanalysisofcalciumspikesinnoisyfluorescentbackground AT alexandrazahradnikova quantitativeanalysisofcalciumspikesinnoisyfluorescentbackground AT ivanzahradnik quantitativeanalysisofcalciumspikesinnoisyfluorescentbackground
_version_	1718423070045110272

Quantitative analysis of calcium spikes in noisy fluorescent background.

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