Biodegradation of 2-hydroxyl-1,4 naphthoquinone (lawsone) by Pseudomonas taiwanensis LH-3 isolated from activated sludge

Abstract 2-hydroxy-1,4 naphthoquinone (lawsone) is widely used and induces environmental pollutions during its production and application. In the present study, a lawsone-degrading bacterium strain, LH-3 was successfully isolated from the activated sludge. Based on the 16S rRNA gene analysis, the st...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Li Yang, Tianming Cai, Dahu Ding, Tianjin Cai, Canlan Jiang, Hua Li, Qian Yang, Liwei Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2dbccb8d94047c2b73565bc6a75c998
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e2dbccb8d94047c2b73565bc6a75c998
record_format	dspace
spelling	oai:doaj.org-article:e2dbccb8d94047c2b73565bc6a75c9982021-12-02T15:05:48ZBiodegradation of 2-hydroxyl-1,4 naphthoquinone (lawsone) by Pseudomonas taiwanensis LH-3 isolated from activated sludge10.1038/s41598-017-06338-12045-2322https://doaj.org/article/e2dbccb8d94047c2b73565bc6a75c9982017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06338-1https://doaj.org/toc/2045-2322Abstract 2-hydroxy-1,4 naphthoquinone (lawsone) is widely used and induces environmental pollutions during its production and application. In the present study, a lawsone-degrading bacterium strain, LH-3 was successfully isolated from the activated sludge. Based on the 16S rRNA gene analysis, the strain LH-3 phylogenetically belonged to the Pseudomonas taiwanensis. It could degrade 200 mg L−1 lawsone completely in 9 h with an inoculum quantity of 1% (v/v). The effects of environmental conditions on the degradation process and the degradation pathway were systematically investigated. LH-3 could maintain its high degradation efficiency under high salt condition. The identified intermediates of salicylic acid, 2-hydroxy-4-oxo-chroman-2-carboxylic acid, and catechol elucidated the potential degradation pathway. Furthermore, the immobilized LH-3 strain cells prepared with alginate gel and biochar performed excellent stability in nine successive degradation runs. It could sucessfully survive in laboratory scale sequencing batch reactor and become to be the dominant species. This study clearly revealed that LH-3 could serve as an attractive candidate for the microbial remediation of lawsone-containing wastewater.Li YangTianming CaiDahu DingTianjin CaiCanlan JiangHua LiQian YangLiwei ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Li Yang Tianming Cai Dahu Ding Tianjin Cai Canlan Jiang Hua Li Qian Yang Liwei Chen Biodegradation of 2-hydroxyl-1,4 naphthoquinone (lawsone) by Pseudomonas taiwanensis LH-3 isolated from activated sludge
description	Abstract 2-hydroxy-1,4 naphthoquinone (lawsone) is widely used and induces environmental pollutions during its production and application. In the present study, a lawsone-degrading bacterium strain, LH-3 was successfully isolated from the activated sludge. Based on the 16S rRNA gene analysis, the strain LH-3 phylogenetically belonged to the Pseudomonas taiwanensis. It could degrade 200 mg L−1 lawsone completely in 9 h with an inoculum quantity of 1% (v/v). The effects of environmental conditions on the degradation process and the degradation pathway were systematically investigated. LH-3 could maintain its high degradation efficiency under high salt condition. The identified intermediates of salicylic acid, 2-hydroxy-4-oxo-chroman-2-carboxylic acid, and catechol elucidated the potential degradation pathway. Furthermore, the immobilized LH-3 strain cells prepared with alginate gel and biochar performed excellent stability in nine successive degradation runs. It could sucessfully survive in laboratory scale sequencing batch reactor and become to be the dominant species. This study clearly revealed that LH-3 could serve as an attractive candidate for the microbial remediation of lawsone-containing wastewater.
format	article
author	Li Yang Tianming Cai Dahu Ding Tianjin Cai Canlan Jiang Hua Li Qian Yang Liwei Chen
author_facet	Li Yang Tianming Cai Dahu Ding Tianjin Cai Canlan Jiang Hua Li Qian Yang Liwei Chen
author_sort	Li Yang
title	Biodegradation of 2-hydroxyl-1,4 naphthoquinone (lawsone) by Pseudomonas taiwanensis LH-3 isolated from activated sludge
title_short	Biodegradation of 2-hydroxyl-1,4 naphthoquinone (lawsone) by Pseudomonas taiwanensis LH-3 isolated from activated sludge
title_full	Biodegradation of 2-hydroxyl-1,4 naphthoquinone (lawsone) by Pseudomonas taiwanensis LH-3 isolated from activated sludge
title_fullStr	Biodegradation of 2-hydroxyl-1,4 naphthoquinone (lawsone) by Pseudomonas taiwanensis LH-3 isolated from activated sludge
title_full_unstemmed	Biodegradation of 2-hydroxyl-1,4 naphthoquinone (lawsone) by Pseudomonas taiwanensis LH-3 isolated from activated sludge
title_sort	biodegradation of 2-hydroxyl-1,4 naphthoquinone (lawsone) by pseudomonas taiwanensis lh-3 isolated from activated sludge
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e2dbccb8d94047c2b73565bc6a75c998
work_keys_str_mv	AT liyang biodegradationof2hydroxyl14naphthoquinonelawsonebypseudomonastaiwanensislh3isolatedfromactivatedsludge AT tianmingcai biodegradationof2hydroxyl14naphthoquinonelawsonebypseudomonastaiwanensislh3isolatedfromactivatedsludge AT dahuding biodegradationof2hydroxyl14naphthoquinonelawsonebypseudomonastaiwanensislh3isolatedfromactivatedsludge AT tianjincai biodegradationof2hydroxyl14naphthoquinonelawsonebypseudomonastaiwanensislh3isolatedfromactivatedsludge AT canlanjiang biodegradationof2hydroxyl14naphthoquinonelawsonebypseudomonastaiwanensislh3isolatedfromactivatedsludge AT huali biodegradationof2hydroxyl14naphthoquinonelawsonebypseudomonastaiwanensislh3isolatedfromactivatedsludge AT qianyang biodegradationof2hydroxyl14naphthoquinonelawsonebypseudomonastaiwanensislh3isolatedfromactivatedsludge AT liweichen biodegradationof2hydroxyl14naphthoquinonelawsonebypseudomonastaiwanensislh3isolatedfromactivatedsludge
_version_	1718388739677356032

Biodegradation of 2-hydroxyl-1,4 naphthoquinone (lawsone) by Pseudomonas taiwanensis LH-3 isolated from activated sludge

Ejemplares similares