High temporal-resolution scanning transmission electron microscopy using sparse-serpentine scan pathways

Abstract Scanning transmission electron microscopy (STEM) provides structural analysis with sub-angstrom resolution. But the pixel-by-pixel scanning process is a limiting factor in acquiring high-speed data. Different strategies have been implemented to increase scanning speeds while at the same tim...

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Autores principales:	Eduardo Ortega, Daniel Nicholls, Nigel D. Browning, Niels de Jonge
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2dbd5205327416b8f2b7b29cfb08690
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spelling	oai:doaj.org-article:e2dbd5205327416b8f2b7b29cfb086902021-11-28T12:16:40ZHigh temporal-resolution scanning transmission electron microscopy using sparse-serpentine scan pathways10.1038/s41598-021-02052-12045-2322https://doaj.org/article/e2dbd5205327416b8f2b7b29cfb086902021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02052-1https://doaj.org/toc/2045-2322Abstract Scanning transmission electron microscopy (STEM) provides structural analysis with sub-angstrom resolution. But the pixel-by-pixel scanning process is a limiting factor in acquiring high-speed data. Different strategies have been implemented to increase scanning speeds while at the same time minimizing beam damage via optimizing the scanning strategy. Here, we achieve the highest possible scanning speed by eliminating the image acquisition dead time induced by the beam flyback time combined with reducing the amount of scanning pixels via sparse imaging. A calibration procedure was developed to compensate for the hysteresis of the magnetic scan coils. A combination of sparse and serpentine scanning routines was tested for a crystalline thin film, gold nanoparticles, and in an in-situ liquid phase STEM experiment. Frame rates of 92, 23 and 5.8 s-1 were achieved for images of a width of 128, 256, and 512 pixels, respectively. The methods described here can be applied to single-particle tracking and analysis of radiation sensitive materials.Eduardo OrtegaDaniel NichollsNigel D. BrowningNiels de JongeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eduardo Ortega Daniel Nicholls Nigel D. Browning Niels de Jonge High temporal-resolution scanning transmission electron microscopy using sparse-serpentine scan pathways
description	Abstract Scanning transmission electron microscopy (STEM) provides structural analysis with sub-angstrom resolution. But the pixel-by-pixel scanning process is a limiting factor in acquiring high-speed data. Different strategies have been implemented to increase scanning speeds while at the same time minimizing beam damage via optimizing the scanning strategy. Here, we achieve the highest possible scanning speed by eliminating the image acquisition dead time induced by the beam flyback time combined with reducing the amount of scanning pixels via sparse imaging. A calibration procedure was developed to compensate for the hysteresis of the magnetic scan coils. A combination of sparse and serpentine scanning routines was tested for a crystalline thin film, gold nanoparticles, and in an in-situ liquid phase STEM experiment. Frame rates of 92, 23 and 5.8 s-1 were achieved for images of a width of 128, 256, and 512 pixels, respectively. The methods described here can be applied to single-particle tracking and analysis of radiation sensitive materials.
format	article
author	Eduardo Ortega Daniel Nicholls Nigel D. Browning Niels de Jonge
author_facet	Eduardo Ortega Daniel Nicholls Nigel D. Browning Niels de Jonge
author_sort	Eduardo Ortega
title	High temporal-resolution scanning transmission electron microscopy using sparse-serpentine scan pathways
title_short	High temporal-resolution scanning transmission electron microscopy using sparse-serpentine scan pathways
title_full	High temporal-resolution scanning transmission electron microscopy using sparse-serpentine scan pathways
title_fullStr	High temporal-resolution scanning transmission electron microscopy using sparse-serpentine scan pathways
title_full_unstemmed	High temporal-resolution scanning transmission electron microscopy using sparse-serpentine scan pathways
title_sort	high temporal-resolution scanning transmission electron microscopy using sparse-serpentine scan pathways
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e2dbd5205327416b8f2b7b29cfb08690
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High temporal-resolution scanning transmission electron microscopy using sparse-serpentine scan pathways

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