Novel IncR/IncP6 Hybrid Plasmid pCRE3-KPC Recovered from a Clinical KPC-2-Producing <named-content content-type="genus-species">Citrobacter braakii</named-content> Isolate

ABSTRACT Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have become widespread in hospitals and the environment. Here, we describe a blaKPC-2-carrying plasmid called pCRE3-KPC, which was recovered from a clinical multidrug-resistant Citrobacter braakii CRE3 strain in China. T...

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Autores principales:	Dandan Dong, Ziqiang Mi, Dujun Li, Mingming Gao, Nan Jia, Manli Li, Yigang Tong, Xianglilan Zhang, Yuanqi Zhu
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Citrobacter braakii blaKPC-2 IncR IncP6 plasmid transposon Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e2dd153d7f33459bb8b654ca38b1cbe4
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spelling	oai:doaj.org-article:e2dd153d7f33459bb8b654ca38b1cbe42021-11-15T15:29:17ZNovel IncR/IncP6 Hybrid Plasmid pCRE3-KPC Recovered from a Clinical KPC-2-Producing <named-content content-type="genus-species">Citrobacter braakii</named-content> Isolate10.1128/mSphere.00891-192379-5042https://doaj.org/article/e2dd153d7f33459bb8b654ca38b1cbe42020-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00891-19https://doaj.org/toc/2379-5042ABSTRACT Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have become widespread in hospitals and the environment. Here, we describe a blaKPC-2-carrying plasmid called pCRE3-KPC, which was recovered from a clinical multidrug-resistant Citrobacter braakii CRE3 strain in China. The complete nucleotide sequence of pCRE3-KPC was determined by combining MiSeq and MinION sequencing and then compared with those of three related plasmids. Plasmid conjugal transfer and electroporation tests, modified carbapenem inactivation method, and bacterial antimicrobial susceptibility test were carried out. We compared this plasmid with three related plasmids to verify that the backbone of pCRE3-KPC was composed of the backbones of the IncR plasmid and IncP6 plasmid. Further bioinformatics analysis showed that pCRE3-KPC carried two resistance-related regions (the blaKPC-2 gene cluster and the aacC2-tmrB-related region). The aacC2-tmrB-related region included two novel insertion sequences (ISCfr28 and ISCfr16). IMPORTANCE Reports of human-pathogenic C. braakii strains, especially of strains showing resistance to carbapenems, are rare. To the best of our knowledge, our results represent the first detection of carbapenemase gene blaKPC-2 in C. braakii strains. In addition, we have studied detailed genetic characteristics of the novel IncR/IncP6 hybrid plasmid pCRE3-KPC, which was isolated from a clinical multidrug-resistant Citrobacter braakii CRE3 strain. Our results may provide further insight into the horizontal transfer of multidrug resistance genes in bacteria and into the genomic diversity and molecular evolution of plasmids.Dandan DongZiqiang MiDujun LiMingming GaoNan JiaManli LiYigang TongXianglilan ZhangYuanqi ZhuAmerican Society for MicrobiologyarticleCitrobacter braakiiblaKPC-2IncRIncP6plasmidtransposonMicrobiologyQR1-502ENmSphere, Vol 5, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Citrobacter braakii blaKPC-2 IncR IncP6 plasmid transposon Microbiology QR1-502
spellingShingle	Citrobacter braakii blaKPC-2 IncR IncP6 plasmid transposon Microbiology QR1-502 Dandan Dong Ziqiang Mi Dujun Li Mingming Gao Nan Jia Manli Li Yigang Tong Xianglilan Zhang Yuanqi Zhu Novel IncR/IncP6 Hybrid Plasmid pCRE3-KPC Recovered from a Clinical KPC-2-Producing <named-content content-type="genus-species">Citrobacter braakii</named-content> Isolate
description	ABSTRACT Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have become widespread in hospitals and the environment. Here, we describe a blaKPC-2-carrying plasmid called pCRE3-KPC, which was recovered from a clinical multidrug-resistant Citrobacter braakii CRE3 strain in China. The complete nucleotide sequence of pCRE3-KPC was determined by combining MiSeq and MinION sequencing and then compared with those of three related plasmids. Plasmid conjugal transfer and electroporation tests, modified carbapenem inactivation method, and bacterial antimicrobial susceptibility test were carried out. We compared this plasmid with three related plasmids to verify that the backbone of pCRE3-KPC was composed of the backbones of the IncR plasmid and IncP6 plasmid. Further bioinformatics analysis showed that pCRE3-KPC carried two resistance-related regions (the blaKPC-2 gene cluster and the aacC2-tmrB-related region). The aacC2-tmrB-related region included two novel insertion sequences (ISCfr28 and ISCfr16). IMPORTANCE Reports of human-pathogenic C. braakii strains, especially of strains showing resistance to carbapenems, are rare. To the best of our knowledge, our results represent the first detection of carbapenemase gene blaKPC-2 in C. braakii strains. In addition, we have studied detailed genetic characteristics of the novel IncR/IncP6 hybrid plasmid pCRE3-KPC, which was isolated from a clinical multidrug-resistant Citrobacter braakii CRE3 strain. Our results may provide further insight into the horizontal transfer of multidrug resistance genes in bacteria and into the genomic diversity and molecular evolution of plasmids.
format	article
author	Dandan Dong Ziqiang Mi Dujun Li Mingming Gao Nan Jia Manli Li Yigang Tong Xianglilan Zhang Yuanqi Zhu
author_facet	Dandan Dong Ziqiang Mi Dujun Li Mingming Gao Nan Jia Manli Li Yigang Tong Xianglilan Zhang Yuanqi Zhu
author_sort	Dandan Dong
title	Novel IncR/IncP6 Hybrid Plasmid pCRE3-KPC Recovered from a Clinical KPC-2-Producing <named-content content-type="genus-species">Citrobacter braakii</named-content> Isolate
title_short	Novel IncR/IncP6 Hybrid Plasmid pCRE3-KPC Recovered from a Clinical KPC-2-Producing <named-content content-type="genus-species">Citrobacter braakii</named-content> Isolate
title_full	Novel IncR/IncP6 Hybrid Plasmid pCRE3-KPC Recovered from a Clinical KPC-2-Producing <named-content content-type="genus-species">Citrobacter braakii</named-content> Isolate
title_fullStr	Novel IncR/IncP6 Hybrid Plasmid pCRE3-KPC Recovered from a Clinical KPC-2-Producing <named-content content-type="genus-species">Citrobacter braakii</named-content> Isolate
title_full_unstemmed	Novel IncR/IncP6 Hybrid Plasmid pCRE3-KPC Recovered from a Clinical KPC-2-Producing <named-content content-type="genus-species">Citrobacter braakii</named-content> Isolate
title_sort	novel incr/incp6 hybrid plasmid pcre3-kpc recovered from a clinical kpc-2-producing <named-content content-type="genus-species">citrobacter braakii</named-content> isolate
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/e2dd153d7f33459bb8b654ca38b1cbe4
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Novel IncR/IncP6 Hybrid Plasmid pCRE3-KPC Recovered from a Clinical KPC-2-Producing <named-content content-type="genus-species">Citrobacter braakii</named-content> Isolate

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