Microbial Antigens Stimulate Metalloprotease-7 Secretion in Human B-Lymphocytes Using mTOR-Dependent and Independent Pathways

Abstract Metalloproteinases (MMPs) contribute to tissue remodeling and acute inflammation not only by degrading extracellular matrix proteins but also by controlling the influx of chemokines through the regulation and shedding of syndecans. B-lymphocytes, in addition to their well-known function as...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mohamed F. Ali, Harika Dasari, Virginia P. Van Keulen, Divi Cornec, George Vasmatzis, Tobias Peikert, Eva M. Carmona
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2dd6df12e6f4057a4bba2a7a7dcea30
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e2dd6df12e6f4057a4bba2a7a7dcea30
record_format	dspace
spelling	oai:doaj.org-article:e2dd6df12e6f4057a4bba2a7a7dcea302021-12-02T15:05:50ZMicrobial Antigens Stimulate Metalloprotease-7 Secretion in Human B-Lymphocytes Using mTOR-Dependent and Independent Pathways10.1038/s41598-017-04199-22045-2322https://doaj.org/article/e2dd6df12e6f4057a4bba2a7a7dcea302017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04199-2https://doaj.org/toc/2045-2322Abstract Metalloproteinases (MMPs) contribute to tissue remodeling and acute inflammation not only by degrading extracellular matrix proteins but also by controlling the influx of chemokines through the regulation and shedding of syndecans. B-lymphocytes, in addition to their well-known function as antibody producing cells, participate in the innate immune response by secreting inflammatory cytokines and chemokines. However, there is little information about the role of B-lymphocytes in the regulation of MMPs; consequently, herein we investigated whether activated human circulating B-lymphocytes contributed to the secretion of MMPs. We demonstrate that B-lymphocytes activated by un-methylated CpG motifs, found in bacterial DNA, and β-glucans, found in the cell wall of fungi, both induced MMP-7. Interestingly, while CpG-stimulated cells activated the mTOR pathway via TLR9 receptor to induced MMP-7, β-glucan-stimulated cells were mTOR-independent and used Dectin-1 receptor. B-lymphocytes did not seem to have a major role in the secretion of tissue inhibitors of metalloproteinases (TIMPs). However, secreted MMP-7 participated in the shedding of Syndecan-4 from the surface of B-lymphocytes. In conclusion, circulating human B-lymphocytes contribute to the regulation of the innate immune system by participating in the secretion of MMP-7 which in turn is important for the shedding of Syndecan-4 in response to infectious stimuli.Mohamed F. AliHarika DasariVirginia P. Van KeulenDivi CornecGeorge VasmatzisTobias PeikertEva M. CarmonaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohamed F. Ali Harika Dasari Virginia P. Van Keulen Divi Cornec George Vasmatzis Tobias Peikert Eva M. Carmona Microbial Antigens Stimulate Metalloprotease-7 Secretion in Human B-Lymphocytes Using mTOR-Dependent and Independent Pathways
description	Abstract Metalloproteinases (MMPs) contribute to tissue remodeling and acute inflammation not only by degrading extracellular matrix proteins but also by controlling the influx of chemokines through the regulation and shedding of syndecans. B-lymphocytes, in addition to their well-known function as antibody producing cells, participate in the innate immune response by secreting inflammatory cytokines and chemokines. However, there is little information about the role of B-lymphocytes in the regulation of MMPs; consequently, herein we investigated whether activated human circulating B-lymphocytes contributed to the secretion of MMPs. We demonstrate that B-lymphocytes activated by un-methylated CpG motifs, found in bacterial DNA, and β-glucans, found in the cell wall of fungi, both induced MMP-7. Interestingly, while CpG-stimulated cells activated the mTOR pathway via TLR9 receptor to induced MMP-7, β-glucan-stimulated cells were mTOR-independent and used Dectin-1 receptor. B-lymphocytes did not seem to have a major role in the secretion of tissue inhibitors of metalloproteinases (TIMPs). However, secreted MMP-7 participated in the shedding of Syndecan-4 from the surface of B-lymphocytes. In conclusion, circulating human B-lymphocytes contribute to the regulation of the innate immune system by participating in the secretion of MMP-7 which in turn is important for the shedding of Syndecan-4 in response to infectious stimuli.
format	article
author	Mohamed F. Ali Harika Dasari Virginia P. Van Keulen Divi Cornec George Vasmatzis Tobias Peikert Eva M. Carmona
author_facet	Mohamed F. Ali Harika Dasari Virginia P. Van Keulen Divi Cornec George Vasmatzis Tobias Peikert Eva M. Carmona
author_sort	Mohamed F. Ali
title	Microbial Antigens Stimulate Metalloprotease-7 Secretion in Human B-Lymphocytes Using mTOR-Dependent and Independent Pathways
title_short	Microbial Antigens Stimulate Metalloprotease-7 Secretion in Human B-Lymphocytes Using mTOR-Dependent and Independent Pathways
title_full	Microbial Antigens Stimulate Metalloprotease-7 Secretion in Human B-Lymphocytes Using mTOR-Dependent and Independent Pathways
title_fullStr	Microbial Antigens Stimulate Metalloprotease-7 Secretion in Human B-Lymphocytes Using mTOR-Dependent and Independent Pathways
title_full_unstemmed	Microbial Antigens Stimulate Metalloprotease-7 Secretion in Human B-Lymphocytes Using mTOR-Dependent and Independent Pathways
title_sort	microbial antigens stimulate metalloprotease-7 secretion in human b-lymphocytes using mtor-dependent and independent pathways
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e2dd6df12e6f4057a4bba2a7a7dcea30
work_keys_str_mv	AT mohamedfali microbialantigensstimulatemetalloprotease7secretioninhumanblymphocytesusingmtordependentandindependentpathways AT harikadasari microbialantigensstimulatemetalloprotease7secretioninhumanblymphocytesusingmtordependentandindependentpathways AT virginiapvankeulen microbialantigensstimulatemetalloprotease7secretioninhumanblymphocytesusingmtordependentandindependentpathways AT divicornec microbialantigensstimulatemetalloprotease7secretioninhumanblymphocytesusingmtordependentandindependentpathways AT georgevasmatzis microbialantigensstimulatemetalloprotease7secretioninhumanblymphocytesusingmtordependentandindependentpathways AT tobiaspeikert microbialantigensstimulatemetalloprotease7secretioninhumanblymphocytesusingmtordependentandindependentpathways AT evamcarmona microbialantigensstimulatemetalloprotease7secretioninhumanblymphocytesusingmtordependentandindependentpathways
_version_	1718388675960635392

Microbial Antigens Stimulate Metalloprotease-7 Secretion in Human B-Lymphocytes Using mTOR-Dependent and Independent Pathways

Ejemplares similares