Caffeine prevents transcription inhibition and P-TEFb/7SK dissociation following UV-induced DNA damage.

<h4>Background</h4>The mechanisms by which DNA damage triggers suppression of transcription of a large number of genes are poorly understood. DNA damage rapidly induces a release of the positive transcription elongation factor b (P-TEFb) from the large inactive multisubunit 7SK snRNP com...

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Autores principales: Giuliana Napolitano, Stefano Amente, Virginia Castiglia, Barbara Gargano, Vera Ruda, Xavier Darzacq, Olivier Bensaude, Barbara Majello, Luigi Lania
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2010
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Acceso en línea:https://doaj.org/article/e2e1c362746c4da2af35c2d2e3ad1ac1
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Sumario:<h4>Background</h4>The mechanisms by which DNA damage triggers suppression of transcription of a large number of genes are poorly understood. DNA damage rapidly induces a release of the positive transcription elongation factor b (P-TEFb) from the large inactive multisubunit 7SK snRNP complex. P-TEFb is required for transcription of most class II genes through stimulation of RNA polymerase II elongation and cotranscriptional pre-mRNA processing.<h4>Methodology/principal findings</h4>We show here that caffeine prevents UV-induced dissociation of P-TEFb as well as transcription inhibition. The caffeine-effect does not involve PI3-kinase-related protein kinases, because inhibition of phosphatidylinositol 3-kinase family members (ATM, ATR and DNA-PK) neither prevents P-TEFb dissociation nor transcription inhibition. Finally, caffeine prevention of transcription inhibition is independent from DNA damage.<h4>Conclusion/significance</h4>Pharmacological prevention of P-TEFb/7SK snRNP dissociation and transcription inhibition following UV-induced DNA damage is correlated.