Caffeine prevents transcription inhibition and P-TEFb/7SK dissociation following UV-induced DNA damage.

<h4>Background</h4>The mechanisms by which DNA damage triggers suppression of transcription of a large number of genes are poorly understood. DNA damage rapidly induces a release of the positive transcription elongation factor b (P-TEFb) from the large inactive multisubunit 7SK snRNP com...

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Autores principales:	Giuliana Napolitano, Stefano Amente, Virginia Castiglia, Barbara Gargano, Vera Ruda, Xavier Darzacq, Olivier Bensaude, Barbara Majello, Luigi Lania
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2e1c362746c4da2af35c2d2e3ad1ac1
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spelling	oai:doaj.org-article:e2e1c362746c4da2af35c2d2e3ad1ac12021-12-02T20:20:38ZCaffeine prevents transcription inhibition and P-TEFb/7SK dissociation following UV-induced DNA damage.1932-620310.1371/journal.pone.0011245https://doaj.org/article/e2e1c362746c4da2af35c2d2e3ad1ac12010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20574533/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The mechanisms by which DNA damage triggers suppression of transcription of a large number of genes are poorly understood. DNA damage rapidly induces a release of the positive transcription elongation factor b (P-TEFb) from the large inactive multisubunit 7SK snRNP complex. P-TEFb is required for transcription of most class II genes through stimulation of RNA polymerase II elongation and cotranscriptional pre-mRNA processing.<h4>Methodology/principal findings</h4>We show here that caffeine prevents UV-induced dissociation of P-TEFb as well as transcription inhibition. The caffeine-effect does not involve PI3-kinase-related protein kinases, because inhibition of phosphatidylinositol 3-kinase family members (ATM, ATR and DNA-PK) neither prevents P-TEFb dissociation nor transcription inhibition. Finally, caffeine prevention of transcription inhibition is independent from DNA damage.<h4>Conclusion/significance</h4>Pharmacological prevention of P-TEFb/7SK snRNP dissociation and transcription inhibition following UV-induced DNA damage is correlated.Giuliana NapolitanoStefano AmenteVirginia CastigliaBarbara GarganoVera RudaXavier DarzacqOlivier BensaudeBarbara MajelloLuigi LaniaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 6, p e11245 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giuliana Napolitano Stefano Amente Virginia Castiglia Barbara Gargano Vera Ruda Xavier Darzacq Olivier Bensaude Barbara Majello Luigi Lania Caffeine prevents transcription inhibition and P-TEFb/7SK dissociation following UV-induced DNA damage.
description	<h4>Background</h4>The mechanisms by which DNA damage triggers suppression of transcription of a large number of genes are poorly understood. DNA damage rapidly induces a release of the positive transcription elongation factor b (P-TEFb) from the large inactive multisubunit 7SK snRNP complex. P-TEFb is required for transcription of most class II genes through stimulation of RNA polymerase II elongation and cotranscriptional pre-mRNA processing.<h4>Methodology/principal findings</h4>We show here that caffeine prevents UV-induced dissociation of P-TEFb as well as transcription inhibition. The caffeine-effect does not involve PI3-kinase-related protein kinases, because inhibition of phosphatidylinositol 3-kinase family members (ATM, ATR and DNA-PK) neither prevents P-TEFb dissociation nor transcription inhibition. Finally, caffeine prevention of transcription inhibition is independent from DNA damage.<h4>Conclusion/significance</h4>Pharmacological prevention of P-TEFb/7SK snRNP dissociation and transcription inhibition following UV-induced DNA damage is correlated.
format	article
author	Giuliana Napolitano Stefano Amente Virginia Castiglia Barbara Gargano Vera Ruda Xavier Darzacq Olivier Bensaude Barbara Majello Luigi Lania
author_facet	Giuliana Napolitano Stefano Amente Virginia Castiglia Barbara Gargano Vera Ruda Xavier Darzacq Olivier Bensaude Barbara Majello Luigi Lania
author_sort	Giuliana Napolitano
title	Caffeine prevents transcription inhibition and P-TEFb/7SK dissociation following UV-induced DNA damage.
title_short	Caffeine prevents transcription inhibition and P-TEFb/7SK dissociation following UV-induced DNA damage.
title_full	Caffeine prevents transcription inhibition and P-TEFb/7SK dissociation following UV-induced DNA damage.
title_fullStr	Caffeine prevents transcription inhibition and P-TEFb/7SK dissociation following UV-induced DNA damage.
title_full_unstemmed	Caffeine prevents transcription inhibition and P-TEFb/7SK dissociation following UV-induced DNA damage.
title_sort	caffeine prevents transcription inhibition and p-tefb/7sk dissociation following uv-induced dna damage.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/e2e1c362746c4da2af35c2d2e3ad1ac1
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Caffeine prevents transcription inhibition and P-TEFb/7SK dissociation following UV-induced DNA damage.

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