Optimized Silica-Binding Peptide-Mediated Delivery of Bactericidal Lysin Efficiently Prevents <i>Staphylococcus aureus</i> from Adhering to Device Surfaces

Staphylococcal-associated device-related infections (DRIs) represent a significant clinical challenge causing major medical and economic sequelae. Bacterial colonization, proliferation, and biofilm formation after adherence to surfaces of the indwelling device are probably the primary cause of DRIs....

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Autores principales:	Wan Yang, Vijay Singh Gondil, Dehua Luo, Jin He, Hongping Wei, Hang Yang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	lysin <i>Staphylococcus aureus</i> silica-binding peptide antimicrobial agents immobilization surface functionalization antimicrobial agents Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/e2e40985d0c243509b0097a50e781d41
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spelling	oai:doaj.org-article:e2e40985d0c243509b0097a50e781d412021-11-25T17:57:39ZOptimized Silica-Binding Peptide-Mediated Delivery of Bactericidal Lysin Efficiently Prevents <i>Staphylococcus aureus</i> from Adhering to Device Surfaces10.3390/ijms2222125441422-00671661-6596https://doaj.org/article/e2e40985d0c243509b0097a50e781d412021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12544https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Staphylococcal-associated device-related infections (DRIs) represent a significant clinical challenge causing major medical and economic sequelae. Bacterial colonization, proliferation, and biofilm formation after adherence to surfaces of the indwelling device are probably the primary cause of DRIs. To address this issue, we incorporated constructs of silica-binding peptide (SiBP) with ClyF, an anti-staphylococcal lysin, into functionalized coatings to impart bactericidal activity against planktonic and sessile <i>Staphylococcus aureus</i>. An optimized construct, SiBP1-ClyF, exhibited improved thermostability and staphylolytic activity compared to its parental lysin ClyF. SiBP1-ClyF-functionalized coatings were efficient in killing MRSA strain N315 (>99.999% within 1 h) and preventing the growth of static and dynamic <i>S. aureus</i> biofilms on various surfaces, including siliconized glass, silicone-coated latex catheter, and silicone catheter. Additionally, SiBP1-ClyF-immobilized surfaces supported normal attachment and growth of mammalian cells. Although the recycling potential and long-term stability of lysin-immobilized surfaces are still affected by the fragility of biological protein molecules, the present study provides a generic strategy for efficient delivery of bactericidal lysin to solid surfaces, which serves as a new approach to prevent the growth of antibiotic-resistant microorganisms on surfaces in hospital settings and could be adapted for other target pathogens as well.Wan YangVijay Singh GondilDehua LuoJin HeHongping WeiHang YangMDPI AGarticlelysin<i>Staphylococcus aureus</i>silica-binding peptideantimicrobial agents immobilizationsurface functionalizationantimicrobial agentsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12544, p 12544 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lysin <i>Staphylococcus aureus</i> silica-binding peptide antimicrobial agents immobilization surface functionalization antimicrobial agents Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	lysin <i>Staphylococcus aureus</i> silica-binding peptide antimicrobial agents immobilization surface functionalization antimicrobial agents Biology (General) QH301-705.5 Chemistry QD1-999 Wan Yang Vijay Singh Gondil Dehua Luo Jin He Hongping Wei Hang Yang Optimized Silica-Binding Peptide-Mediated Delivery of Bactericidal Lysin Efficiently Prevents <i>Staphylococcus aureus</i> from Adhering to Device Surfaces
description	Staphylococcal-associated device-related infections (DRIs) represent a significant clinical challenge causing major medical and economic sequelae. Bacterial colonization, proliferation, and biofilm formation after adherence to surfaces of the indwelling device are probably the primary cause of DRIs. To address this issue, we incorporated constructs of silica-binding peptide (SiBP) with ClyF, an anti-staphylococcal lysin, into functionalized coatings to impart bactericidal activity against planktonic and sessile <i>Staphylococcus aureus</i>. An optimized construct, SiBP1-ClyF, exhibited improved thermostability and staphylolytic activity compared to its parental lysin ClyF. SiBP1-ClyF-functionalized coatings were efficient in killing MRSA strain N315 (>99.999% within 1 h) and preventing the growth of static and dynamic <i>S. aureus</i> biofilms on various surfaces, including siliconized glass, silicone-coated latex catheter, and silicone catheter. Additionally, SiBP1-ClyF-immobilized surfaces supported normal attachment and growth of mammalian cells. Although the recycling potential and long-term stability of lysin-immobilized surfaces are still affected by the fragility of biological protein molecules, the present study provides a generic strategy for efficient delivery of bactericidal lysin to solid surfaces, which serves as a new approach to prevent the growth of antibiotic-resistant microorganisms on surfaces in hospital settings and could be adapted for other target pathogens as well.
format	article
author	Wan Yang Vijay Singh Gondil Dehua Luo Jin He Hongping Wei Hang Yang
author_facet	Wan Yang Vijay Singh Gondil Dehua Luo Jin He Hongping Wei Hang Yang
author_sort	Wan Yang
title	Optimized Silica-Binding Peptide-Mediated Delivery of Bactericidal Lysin Efficiently Prevents <i>Staphylococcus aureus</i> from Adhering to Device Surfaces
title_short	Optimized Silica-Binding Peptide-Mediated Delivery of Bactericidal Lysin Efficiently Prevents <i>Staphylococcus aureus</i> from Adhering to Device Surfaces
title_full	Optimized Silica-Binding Peptide-Mediated Delivery of Bactericidal Lysin Efficiently Prevents <i>Staphylococcus aureus</i> from Adhering to Device Surfaces
title_fullStr	Optimized Silica-Binding Peptide-Mediated Delivery of Bactericidal Lysin Efficiently Prevents <i>Staphylococcus aureus</i> from Adhering to Device Surfaces
title_full_unstemmed	Optimized Silica-Binding Peptide-Mediated Delivery of Bactericidal Lysin Efficiently Prevents <i>Staphylococcus aureus</i> from Adhering to Device Surfaces
title_sort	optimized silica-binding peptide-mediated delivery of bactericidal lysin efficiently prevents <i>staphylococcus aureus</i> from adhering to device surfaces
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e2e40985d0c243509b0097a50e781d41
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Optimized Silica-Binding Peptide-Mediated Delivery of Bactericidal Lysin Efficiently Prevents <i>Staphylococcus aureus</i> from Adhering to Device Surfaces

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