Analysis of MicroRNA Profile Alterations in Extracellular Vesicles From Mesenchymal Stromal Cells Overexpressing Stem Cell Factor

Mesenchymal stem/stromal cells (MSCs) represent a promising tool to treat cardiovascular diseases. One mode of action through which MSCs exert their protective effects is secretion of extracellular vesicles (EVs). Recently, we demonstrated that rat adipose-derived MSC-overexpressing stem cell factor...

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Autores principales:	Ekaterina Zubkova, Evgeniy Evtushenko, Irina Beloglazova, German Osmak, Phillip Koshkin, Alexander Moschenko, Mikhail Menshikov, Yelena Parfyonova
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	mesenchymal stromal cells extracellular vesicles adeno-associated viral vectors miRNA stress Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e2f417c216fc4d05b5a87b1345c969f1
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spelling	oai:doaj.org-article:e2f417c216fc4d05b5a87b1345c969f12021-11-15T14:26:09ZAnalysis of MicroRNA Profile Alterations in Extracellular Vesicles From Mesenchymal Stromal Cells Overexpressing Stem Cell Factor2296-634X10.3389/fcell.2021.754025https://doaj.org/article/e2f417c216fc4d05b5a87b1345c969f12021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.754025/fullhttps://doaj.org/toc/2296-634XMesenchymal stem/stromal cells (MSCs) represent a promising tool to treat cardiovascular diseases. One mode of action through which MSCs exert their protective effects is secretion of extracellular vesicles (EVs). Recently, we demonstrated that rat adipose-derived MSC-overexpressing stem cell factor (SCF) can induce endogenous regenerative processes and improve cardiac function. In the present work, we isolated EVs from intact, GFP- or SCF-overexpressing rat MSC and analyzed microarray datasets of their miRNA cargo. We uncovered a total of 95 differentially expressed miRNAs. We did not observe significant differences between EVs from GFP-MSC and SCF-MSC that may indicate intrinsic changes in MSC after viral transduction. About 80 miRNAs were downregulated in EVs from both SCF- or GFP-MSC. We assembled the miRNA-based network and found several nodes of target genes among which Vim Sept3 and Vsnl1 are involved in regulation of cellular migration that is consistent with our previous EVs data. Topological analyses of the network also revealed that among the downregulated miRNA-rno-miRNA-128-3p that regulates plenty of targets is presumably associated with chemokine signaling pathways. Overall, our data suggest that genetic modification of MSC has a great impact on their miRNA composition and provide novel insights into the regulatory networks underlying EV effects.Ekaterina ZubkovaEvgeniy EvtushenkoIrina BeloglazovaGerman OsmakPhillip KoshkinAlexander MoschenkoMikhail MenshikovYelena ParfyonovaFrontiers Media S.A.articlemesenchymal stromal cellsextracellular vesiclesadeno-associated viral vectorsmiRNAstressBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mesenchymal stromal cells extracellular vesicles adeno-associated viral vectors miRNA stress Biology (General) QH301-705.5
spellingShingle	mesenchymal stromal cells extracellular vesicles adeno-associated viral vectors miRNA stress Biology (General) QH301-705.5 Ekaterina Zubkova Evgeniy Evtushenko Irina Beloglazova German Osmak Phillip Koshkin Alexander Moschenko Mikhail Menshikov Yelena Parfyonova Analysis of MicroRNA Profile Alterations in Extracellular Vesicles From Mesenchymal Stromal Cells Overexpressing Stem Cell Factor
description	Mesenchymal stem/stromal cells (MSCs) represent a promising tool to treat cardiovascular diseases. One mode of action through which MSCs exert their protective effects is secretion of extracellular vesicles (EVs). Recently, we demonstrated that rat adipose-derived MSC-overexpressing stem cell factor (SCF) can induce endogenous regenerative processes and improve cardiac function. In the present work, we isolated EVs from intact, GFP- or SCF-overexpressing rat MSC and analyzed microarray datasets of their miRNA cargo. We uncovered a total of 95 differentially expressed miRNAs. We did not observe significant differences between EVs from GFP-MSC and SCF-MSC that may indicate intrinsic changes in MSC after viral transduction. About 80 miRNAs were downregulated in EVs from both SCF- or GFP-MSC. We assembled the miRNA-based network and found several nodes of target genes among which Vim Sept3 and Vsnl1 are involved in regulation of cellular migration that is consistent with our previous EVs data. Topological analyses of the network also revealed that among the downregulated miRNA-rno-miRNA-128-3p that regulates plenty of targets is presumably associated with chemokine signaling pathways. Overall, our data suggest that genetic modification of MSC has a great impact on their miRNA composition and provide novel insights into the regulatory networks underlying EV effects.
format	article
author	Ekaterina Zubkova Evgeniy Evtushenko Irina Beloglazova German Osmak Phillip Koshkin Alexander Moschenko Mikhail Menshikov Yelena Parfyonova
author_facet	Ekaterina Zubkova Evgeniy Evtushenko Irina Beloglazova German Osmak Phillip Koshkin Alexander Moschenko Mikhail Menshikov Yelena Parfyonova
author_sort	Ekaterina Zubkova
title	Analysis of MicroRNA Profile Alterations in Extracellular Vesicles From Mesenchymal Stromal Cells Overexpressing Stem Cell Factor
title_short	Analysis of MicroRNA Profile Alterations in Extracellular Vesicles From Mesenchymal Stromal Cells Overexpressing Stem Cell Factor
title_full	Analysis of MicroRNA Profile Alterations in Extracellular Vesicles From Mesenchymal Stromal Cells Overexpressing Stem Cell Factor
title_fullStr	Analysis of MicroRNA Profile Alterations in Extracellular Vesicles From Mesenchymal Stromal Cells Overexpressing Stem Cell Factor
title_full_unstemmed	Analysis of MicroRNA Profile Alterations in Extracellular Vesicles From Mesenchymal Stromal Cells Overexpressing Stem Cell Factor
title_sort	analysis of microrna profile alterations in extracellular vesicles from mesenchymal stromal cells overexpressing stem cell factor
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e2f417c216fc4d05b5a87b1345c969f1
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Analysis of MicroRNA Profile Alterations in Extracellular Vesicles From Mesenchymal Stromal Cells Overexpressing Stem Cell Factor

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