The role of bacterial urease activity on the uniformity of carbonate precipitation profiles of bio-treated coarse sand specimens

Abstract Protocols for microbially induced carbonate precipitation (MICP) have been extensively studied in the literature to optimise the process with regard to the amount of injected chemicals, the ratio of urea to calcium chloride, the method of injection and injection intervals, and the populatio...

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Autores principales:	Charalampos Konstantinou, Yuze Wang, Giovanna Biscontin, Kenichi Soga
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2f4a822879546508f9f1a238acbd284
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spelling	oai:doaj.org-article:e2f4a822879546508f9f1a238acbd2842021-12-02T11:39:47ZThe role of bacterial urease activity on the uniformity of carbonate precipitation profiles of bio-treated coarse sand specimens10.1038/s41598-021-85712-62045-2322https://doaj.org/article/e2f4a822879546508f9f1a238acbd2842021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85712-6https://doaj.org/toc/2045-2322Abstract Protocols for microbially induced carbonate precipitation (MICP) have been extensively studied in the literature to optimise the process with regard to the amount of injected chemicals, the ratio of urea to calcium chloride, the method of injection and injection intervals, and the population of the bacteria, usually using fine- to medium-grained poorly graded sands. This study assesses the effect of varying urease activities, which have not been studied systematically, and population densities of the bacteria on the uniformity of cementation in very coarse sands (considered poor candidates for treatment). A procedure for producing bacteria with the desired urease activities was developed and qPCR tests were conducted to measure the counts of the RNA of the Ure-C genes. Sand biocementaton experiments followed, showing that slower rates of MICP reactions promote more effective and uniform cementation. Lowering urease activity, in particular, results in progressively more uniformly cemented samples and it is proven to be effective enough when its value is less than 10 mmol/L/h. The work presented highlights the importance of urease activity in controlling the quality and quantity of calcium carbonate cements.Charalampos KonstantinouYuze WangGiovanna BiscontinKenichi SogaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charalampos Konstantinou Yuze Wang Giovanna Biscontin Kenichi Soga The role of bacterial urease activity on the uniformity of carbonate precipitation profiles of bio-treated coarse sand specimens
description	Abstract Protocols for microbially induced carbonate precipitation (MICP) have been extensively studied in the literature to optimise the process with regard to the amount of injected chemicals, the ratio of urea to calcium chloride, the method of injection and injection intervals, and the population of the bacteria, usually using fine- to medium-grained poorly graded sands. This study assesses the effect of varying urease activities, which have not been studied systematically, and population densities of the bacteria on the uniformity of cementation in very coarse sands (considered poor candidates for treatment). A procedure for producing bacteria with the desired urease activities was developed and qPCR tests were conducted to measure the counts of the RNA of the Ure-C genes. Sand biocementaton experiments followed, showing that slower rates of MICP reactions promote more effective and uniform cementation. Lowering urease activity, in particular, results in progressively more uniformly cemented samples and it is proven to be effective enough when its value is less than 10 mmol/L/h. The work presented highlights the importance of urease activity in controlling the quality and quantity of calcium carbonate cements.
format	article
author	Charalampos Konstantinou Yuze Wang Giovanna Biscontin Kenichi Soga
author_facet	Charalampos Konstantinou Yuze Wang Giovanna Biscontin Kenichi Soga
author_sort	Charalampos Konstantinou
title	The role of bacterial urease activity on the uniformity of carbonate precipitation profiles of bio-treated coarse sand specimens
title_short	The role of bacterial urease activity on the uniformity of carbonate precipitation profiles of bio-treated coarse sand specimens
title_full	The role of bacterial urease activity on the uniformity of carbonate precipitation profiles of bio-treated coarse sand specimens
title_fullStr	The role of bacterial urease activity on the uniformity of carbonate precipitation profiles of bio-treated coarse sand specimens
title_full_unstemmed	The role of bacterial urease activity on the uniformity of carbonate precipitation profiles of bio-treated coarse sand specimens
title_sort	role of bacterial urease activity on the uniformity of carbonate precipitation profiles of bio-treated coarse sand specimens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e2f4a822879546508f9f1a238acbd284
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The role of bacterial urease activity on the uniformity of carbonate precipitation profiles of bio-treated coarse sand specimens

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