Gram-Negative Bacterial Lipopolysaccharide Promotes Tumor Cell Proliferation in Breast Implant-Associated Anaplastic Large-Cell Lymphoma

Breast implant-associated anaplastic large-cell lymphoma (BIA-ALCL) is a distinct malignancy associated with textured breast implants. We investigated whether bacteria could trigger the activation and multiplication of BIA-ALCL cells in vitro. BIA-ALCL patient-derived BIA-ALCL tumor cells, BIA-ALCL...

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Autores principales:	Maria Mempin, Honghua Hu, Karen Vickery, Marshall E. Kadin, H. Miles Prince, Nicola Kouttab, John W. Morgan, William P. Adams, Anand K. Deva
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	breast implant-associated anaplastic large-cell lymphoma lipopolysaccharide tumor cell proliferation T-cells’ malignancy Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/e2f79716593c4317a03ff18b5949a9e2
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spelling	oai:doaj.org-article:e2f79716593c4317a03ff18b5949a9e22021-11-11T15:27:41ZGram-Negative Bacterial Lipopolysaccharide Promotes Tumor Cell Proliferation in Breast Implant-Associated Anaplastic Large-Cell Lymphoma10.3390/cancers132152982072-6694https://doaj.org/article/e2f79716593c4317a03ff18b5949a9e22021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5298https://doaj.org/toc/2072-6694Breast implant-associated anaplastic large-cell lymphoma (BIA-ALCL) is a distinct malignancy associated with textured breast implants. We investigated whether bacteria could trigger the activation and multiplication of BIA-ALCL cells in vitro. BIA-ALCL patient-derived BIA-ALCL tumor cells, BIA-ALCL cell lines, cutaneous ALCL cell lines, an immortal T-cell line (MT-4), and peripheral blood mononuclear cells (PBMC) from BIA-ALCL, capsular contracture, and primary augmentation patients were studied. Cells were subjected to various mitogenic stimulation assays including plant phytohemagglutinin (PHA), Gram-negative bacterial lipopolysaccharide (LPS), Staphylococcal superantigens enterotoxin A (SEA), toxic shock syndrome toxin-1 (TSST-1), or sterilized implant shells. Patient-derived BIA-ALCL tumor cells and BIA-ALCL cell lines showed a unique response to LPS stimulation. This response was dampened significantly in the presence of a Toll-like receptor 4 (TLR4) inhibitor peptide. In contrast, cutaneous ALCL cells, MT-4, and PBMC cells from all patients responded significantly more to PHA, SEA, and TSST-1 than to LPS. Breast implant shells of all surface grades alone did not produce a proliferative response of BIA-ALCL cells, indicating the breast implant does not act as a pro-inflammatory stimulant. These findings indicate a possible novel pathway for LPS to promote BIA-ALCL cell proliferation via a TLR4 receptor-mediated bacterial transformation of T-cells into malignancy.Maria MempinHonghua HuKaren VickeryMarshall E. KadinH. Miles PrinceNicola KouttabJohn W. MorganWilliam P. AdamsAnand K. DevaMDPI AGarticlebreast implant-associated anaplastic large-cell lymphomalipopolysaccharidetumor cellproliferationT-cells’ malignancyNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5298, p 5298 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast implant-associated anaplastic large-cell lymphoma lipopolysaccharide tumor cell proliferation T-cells’ malignancy Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	breast implant-associated anaplastic large-cell lymphoma lipopolysaccharide tumor cell proliferation T-cells’ malignancy Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Maria Mempin Honghua Hu Karen Vickery Marshall E. Kadin H. Miles Prince Nicola Kouttab John W. Morgan William P. Adams Anand K. Deva Gram-Negative Bacterial Lipopolysaccharide Promotes Tumor Cell Proliferation in Breast Implant-Associated Anaplastic Large-Cell Lymphoma
description	Breast implant-associated anaplastic large-cell lymphoma (BIA-ALCL) is a distinct malignancy associated with textured breast implants. We investigated whether bacteria could trigger the activation and multiplication of BIA-ALCL cells in vitro. BIA-ALCL patient-derived BIA-ALCL tumor cells, BIA-ALCL cell lines, cutaneous ALCL cell lines, an immortal T-cell line (MT-4), and peripheral blood mononuclear cells (PBMC) from BIA-ALCL, capsular contracture, and primary augmentation patients were studied. Cells were subjected to various mitogenic stimulation assays including plant phytohemagglutinin (PHA), Gram-negative bacterial lipopolysaccharide (LPS), Staphylococcal superantigens enterotoxin A (SEA), toxic shock syndrome toxin-1 (TSST-1), or sterilized implant shells. Patient-derived BIA-ALCL tumor cells and BIA-ALCL cell lines showed a unique response to LPS stimulation. This response was dampened significantly in the presence of a Toll-like receptor 4 (TLR4) inhibitor peptide. In contrast, cutaneous ALCL cells, MT-4, and PBMC cells from all patients responded significantly more to PHA, SEA, and TSST-1 than to LPS. Breast implant shells of all surface grades alone did not produce a proliferative response of BIA-ALCL cells, indicating the breast implant does not act as a pro-inflammatory stimulant. These findings indicate a possible novel pathway for LPS to promote BIA-ALCL cell proliferation via a TLR4 receptor-mediated bacterial transformation of T-cells into malignancy.
format	article
author	Maria Mempin Honghua Hu Karen Vickery Marshall E. Kadin H. Miles Prince Nicola Kouttab John W. Morgan William P. Adams Anand K. Deva
author_facet	Maria Mempin Honghua Hu Karen Vickery Marshall E. Kadin H. Miles Prince Nicola Kouttab John W. Morgan William P. Adams Anand K. Deva
author_sort	Maria Mempin
title	Gram-Negative Bacterial Lipopolysaccharide Promotes Tumor Cell Proliferation in Breast Implant-Associated Anaplastic Large-Cell Lymphoma
title_short	Gram-Negative Bacterial Lipopolysaccharide Promotes Tumor Cell Proliferation in Breast Implant-Associated Anaplastic Large-Cell Lymphoma
title_full	Gram-Negative Bacterial Lipopolysaccharide Promotes Tumor Cell Proliferation in Breast Implant-Associated Anaplastic Large-Cell Lymphoma
title_fullStr	Gram-Negative Bacterial Lipopolysaccharide Promotes Tumor Cell Proliferation in Breast Implant-Associated Anaplastic Large-Cell Lymphoma
title_full_unstemmed	Gram-Negative Bacterial Lipopolysaccharide Promotes Tumor Cell Proliferation in Breast Implant-Associated Anaplastic Large-Cell Lymphoma
title_sort	gram-negative bacterial lipopolysaccharide promotes tumor cell proliferation in breast implant-associated anaplastic large-cell lymphoma
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e2f79716593c4317a03ff18b5949a9e2
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Gram-Negative Bacterial Lipopolysaccharide Promotes Tumor Cell Proliferation in Breast Implant-Associated Anaplastic Large-Cell Lymphoma

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