Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging

Pramod K Avti, Balaji SitharamanDepartment of Biomedical Engineering, Stony Brook University, Stony Brook, NY, USAAbstract: Lanthanoid-based optical probes with excitation wavelengths in the ultra-violet (UV) range (300&ndash;325 nm) have been widely developed as imaging probes. Efficient ce...

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Autores principales:	Avti PK, Sitharaman B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e303d308aa964ab198b9b7a25a025fe3
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spelling	oai:doaj.org-article:e303d308aa964ab198b9b7a25a025fe32021-12-02T05:09:40ZLuminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging1176-91141178-2013https://doaj.org/article/e303d308aa964ab198b9b7a25a025fe32012-04-01T00:00:00Zhttp://www.dovepress.com/luminescent-single-walled-carbon-nanotube-sensitized-europium-nanoprob-a9706https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Pramod K Avti, Balaji SitharamanDepartment of Biomedical Engineering, Stony Brook University, Stony Brook, NY, USAAbstract: Lanthanoid-based optical probes with excitation wavelengths in the ultra-violet (UV) range (300&ndash;325 nm) have been widely developed as imaging probes. Efficient cellular imaging requires that lanthanoid optical probes be excited at visible wavelengths, to avoid UV damage to cells. The efficacy of europium-catalyzed single-walled carbon nanotubes (Eu-SWCNTs), as visible nanoprobes for cellular imaging, is reported in this study. Confocal fluorescence microscopy images of breast cancer cells (SK-BR-3 and MCF-7) and normal cells (NIH 3T3), treated with Eu-SWCNT at 0.2 &micro;g/mL concentration, showed bright red luminescence after excitation at 365 nm and 458 nm wavelengths. Cell viability analysis showed no cytotoxic effects after the incubation of cells with Eu-SWCNTs at this concentration. Eu-SWCNT uptake is via the endocytosis mechanism. Labeling efficiency, defined as the percentage of incubated cells that uptake Eu-SWCNT, was 95%&ndash;100% for all cell types. The average cellular uptake concentration was 6.68 ng Eu per cell. Intracellular localization was further corroborated by transmission electron microscopy and Raman microscopy. The results indicate that Eu-SWCNT shows potential as a novel cellular imaging probe, wherein SWCNT sensitizes Eu3+ ions to allow excitation at visible wavelengths, and stable time-resolved red emission. The ability to functionalize biomolecules on the exterior surface of Eu-SWCNT makes it an excellent candidate for targeted cellular imaging.Keywords: cellular imaging, confocal microscopy, europium, single-walled carbon nanotubes, luminescence, visible excitationAvti PKSitharaman BDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1953-1964 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Avti PK Sitharaman B Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
description	Pramod K Avti, Balaji SitharamanDepartment of Biomedical Engineering, Stony Brook University, Stony Brook, NY, USAAbstract: Lanthanoid-based optical probes with excitation wavelengths in the ultra-violet (UV) range (300&ndash;325 nm) have been widely developed as imaging probes. Efficient cellular imaging requires that lanthanoid optical probes be excited at visible wavelengths, to avoid UV damage to cells. The efficacy of europium-catalyzed single-walled carbon nanotubes (Eu-SWCNTs), as visible nanoprobes for cellular imaging, is reported in this study. Confocal fluorescence microscopy images of breast cancer cells (SK-BR-3 and MCF-7) and normal cells (NIH 3T3), treated with Eu-SWCNT at 0.2 &micro;g/mL concentration, showed bright red luminescence after excitation at 365 nm and 458 nm wavelengths. Cell viability analysis showed no cytotoxic effects after the incubation of cells with Eu-SWCNTs at this concentration. Eu-SWCNT uptake is via the endocytosis mechanism. Labeling efficiency, defined as the percentage of incubated cells that uptake Eu-SWCNT, was 95%&ndash;100% for all cell types. The average cellular uptake concentration was 6.68 ng Eu per cell. Intracellular localization was further corroborated by transmission electron microscopy and Raman microscopy. The results indicate that Eu-SWCNT shows potential as a novel cellular imaging probe, wherein SWCNT sensitizes Eu3+ ions to allow excitation at visible wavelengths, and stable time-resolved red emission. The ability to functionalize biomolecules on the exterior surface of Eu-SWCNT makes it an excellent candidate for targeted cellular imaging.Keywords: cellular imaging, confocal microscopy, europium, single-walled carbon nanotubes, luminescence, visible excitation
format	article
author	Avti PK Sitharaman B
author_facet	Avti PK Sitharaman B
author_sort	Avti PK
title	Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
title_short	Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
title_full	Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
title_fullStr	Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
title_full_unstemmed	Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
title_sort	luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/e303d308aa964ab198b9b7a25a025fe3
work_keys_str_mv	AT avtipk luminescentsinglewalledcarbonnanotubesensitizedeuropiumnanoprobesforcellularimaging AT sitharamanb luminescentsinglewalledcarbonnanotubesensitizedeuropiumnanoprobesforcellularimaging
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Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging

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