Neurotensin and CRH interactions augment human mast cell activation.

Stress affects immunity, but the mechanism is not known. Neurotensin (NT) and corticotropin-releasing hormone (CRH) are secreted under stress in various tissues, and have immunomodulatory actions. We had previously shown that NT augments the ability of CRH to increase mast cell-dependent skin vascul...

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Autores principales:	Konstantinos-Dionysios Alysandratos, Shahrzad Asadi, Asimenia Angelidou, Bodi Zhang, Nikolaos Sismanopoulos, Hailing Yang, Agatha Critchfield, Theoharis C Theoharides
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e3090a62aa70494dba26945830315b3a
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spelling	oai:doaj.org-article:e3090a62aa70494dba26945830315b3a2021-11-18T08:08:51ZNeurotensin and CRH interactions augment human mast cell activation.1932-620310.1371/journal.pone.0048934https://doaj.org/article/e3090a62aa70494dba26945830315b3a2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23155429/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Stress affects immunity, but the mechanism is not known. Neurotensin (NT) and corticotropin-releasing hormone (CRH) are secreted under stress in various tissues, and have immunomodulatory actions. We had previously shown that NT augments the ability of CRH to increase mast cell-dependent skin vascular permeability in rodents. Here we show that NT triggered human mast cell degranulation and significantly augmented CRH-induced vascular endothelial growth factor (VEGF) release. Investigation of various signaling molecules indicated that only NF-κB activation was involved. These effects were blocked by pretreatment with the NTR antagonist SR48692. NT induced expression of CRH receptor-1 (CRHR-1), as shown by Western blot and FACS analysis. Interestingly, CRH also induced NTR gene and protein expression. These results indicate unique interactions among NT, CRH, and mast cells that may contribute to auto-immune and inflammatory diseases that worsen with stress.Konstantinos-Dionysios AlysandratosShahrzad AsadiAsimenia AngelidouBodi ZhangNikolaos SismanopoulosHailing YangAgatha CritchfieldTheoharis C TheoharidesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e48934 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Konstantinos-Dionysios Alysandratos Shahrzad Asadi Asimenia Angelidou Bodi Zhang Nikolaos Sismanopoulos Hailing Yang Agatha Critchfield Theoharis C Theoharides Neurotensin and CRH interactions augment human mast cell activation.
description	Stress affects immunity, but the mechanism is not known. Neurotensin (NT) and corticotropin-releasing hormone (CRH) are secreted under stress in various tissues, and have immunomodulatory actions. We had previously shown that NT augments the ability of CRH to increase mast cell-dependent skin vascular permeability in rodents. Here we show that NT triggered human mast cell degranulation and significantly augmented CRH-induced vascular endothelial growth factor (VEGF) release. Investigation of various signaling molecules indicated that only NF-κB activation was involved. These effects were blocked by pretreatment with the NTR antagonist SR48692. NT induced expression of CRH receptor-1 (CRHR-1), as shown by Western blot and FACS analysis. Interestingly, CRH also induced NTR gene and protein expression. These results indicate unique interactions among NT, CRH, and mast cells that may contribute to auto-immune and inflammatory diseases that worsen with stress.
format	article
author	Konstantinos-Dionysios Alysandratos Shahrzad Asadi Asimenia Angelidou Bodi Zhang Nikolaos Sismanopoulos Hailing Yang Agatha Critchfield Theoharis C Theoharides
author_facet	Konstantinos-Dionysios Alysandratos Shahrzad Asadi Asimenia Angelidou Bodi Zhang Nikolaos Sismanopoulos Hailing Yang Agatha Critchfield Theoharis C Theoharides
author_sort	Konstantinos-Dionysios Alysandratos
title	Neurotensin and CRH interactions augment human mast cell activation.
title_short	Neurotensin and CRH interactions augment human mast cell activation.
title_full	Neurotensin and CRH interactions augment human mast cell activation.
title_fullStr	Neurotensin and CRH interactions augment human mast cell activation.
title_full_unstemmed	Neurotensin and CRH interactions augment human mast cell activation.
title_sort	neurotensin and crh interactions augment human mast cell activation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e3090a62aa70494dba26945830315b3a
work_keys_str_mv	AT konstantinosdionysiosalysandratos neurotensinandcrhinteractionsaugmenthumanmastcellactivation AT shahrzadasadi neurotensinandcrhinteractionsaugmenthumanmastcellactivation AT asimeniaangelidou neurotensinandcrhinteractionsaugmenthumanmastcellactivation AT bodizhang neurotensinandcrhinteractionsaugmenthumanmastcellactivation AT nikolaossismanopoulos neurotensinandcrhinteractionsaugmenthumanmastcellactivation AT hailingyang neurotensinandcrhinteractionsaugmenthumanmastcellactivation AT agathacritchfield neurotensinandcrhinteractionsaugmenthumanmastcellactivation AT theoharisctheoharides neurotensinandcrhinteractionsaugmenthumanmastcellactivation
_version_	1718422201415237632

Neurotensin and CRH interactions augment human mast cell activation.

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