Genetic diversity and drug susceptibility of Mycobacterium tuberculosis in a city with a high prevalence of drug resistant tuberculosis from Southeast of Mexico

Abstract Background Mexico is on the top five countries with the highest number of TB cases in America continent, nevertheless, information about genotypes circulating is practically unknown. Considering the above this study aims to characterize the genetic diversity of TB in the city of Veracruz, M...

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Autores principales:	Roberto Zenteno-Cuevas, Daniela Munro-Rojas, Damián Pérez-Martínez, Esdras Fernandez-Morales, Ana C. Jimenez-Ruano, Hilda Montero, Leila Escobar, Everest de Igartua, Ángel Trigos, Javier Fuentes-Dominguez
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Spoligotyping MIRU-VNTR Genotyping Tuberculosis Mexico Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/e30d0e01d06c497f99ce8e7345497e35
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spelling	oai:doaj.org-article:e30d0e01d06c497f99ce8e7345497e352021-12-05T12:26:16ZGenetic diversity and drug susceptibility of Mycobacterium tuberculosis in a city with a high prevalence of drug resistant tuberculosis from Southeast of Mexico10.1186/s12879-021-06904-z1471-2334https://doaj.org/article/e30d0e01d06c497f99ce8e7345497e352021-11-01T00:00:00Zhttps://doi.org/10.1186/s12879-021-06904-zhttps://doaj.org/toc/1471-2334Abstract Background Mexico is on the top five countries with the highest number of TB cases in America continent, nevertheless, information about genotypes circulating is practically unknown. Considering the above this study aims to characterize the genetic diversity of TB in the city of Veracruz, México. Methods A cross-sectional study was conducted among positive smear samples from patients living in Veracruz City, samples were cultured, and first-line drug profiles determined. Genotyping was made by spoligotyping and MIRU-VNTR 24 loci. Associations of lineages, clusters, and variables were also analyzed. Results Among the 202 isolates analyzed resistance to at least one drug was observed in 60 (30%) isolates and 41(20%) were multidrug-resistant. Three major lineages were identified: L4/Euro-American (88%), L1/Indo-Oceanic (9%), and L2/East Asian (3%). The Euro-American lineage included more than six sublineages, the most abundant were: H (32%), T (23%), LAM (18%), and X (12%). 140 isolates (70%) were placed in 42 SITs patterns. Conclusions These results provide the first baseline data on the genetic structure of TB in the city of Veracruz. Sublineages H, X and LAM were predominant; however, it was founded an important diversity of genotypes that could contribute to the dispersion of TB and explain the high prevalence. This information might be useful for the development of further interventions to reduce impact of TB.Roberto Zenteno-CuevasDaniela Munro-RojasDamián Pérez-MartínezEsdras Fernandez-MoralesAna C. Jimenez-RuanoHilda MonteroLeila EscobarEverest de IgartuaÁngel TrigosJavier Fuentes-DominguezBMCarticleSpoligotypingMIRU-VNTRGenotypingTuberculosisMexicoInfectious and parasitic diseasesRC109-216ENBMC Infectious Diseases, Vol 21, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Spoligotyping MIRU-VNTR Genotyping Tuberculosis Mexico Infectious and parasitic diseases RC109-216
spellingShingle	Spoligotyping MIRU-VNTR Genotyping Tuberculosis Mexico Infectious and parasitic diseases RC109-216 Roberto Zenteno-Cuevas Daniela Munro-Rojas Damián Pérez-Martínez Esdras Fernandez-Morales Ana C. Jimenez-Ruano Hilda Montero Leila Escobar Everest de Igartua Ángel Trigos Javier Fuentes-Dominguez Genetic diversity and drug susceptibility of Mycobacterium tuberculosis in a city with a high prevalence of drug resistant tuberculosis from Southeast of Mexico
description	Abstract Background Mexico is on the top five countries with the highest number of TB cases in America continent, nevertheless, information about genotypes circulating is practically unknown. Considering the above this study aims to characterize the genetic diversity of TB in the city of Veracruz, México. Methods A cross-sectional study was conducted among positive smear samples from patients living in Veracruz City, samples were cultured, and first-line drug profiles determined. Genotyping was made by spoligotyping and MIRU-VNTR 24 loci. Associations of lineages, clusters, and variables were also analyzed. Results Among the 202 isolates analyzed resistance to at least one drug was observed in 60 (30%) isolates and 41(20%) were multidrug-resistant. Three major lineages were identified: L4/Euro-American (88%), L1/Indo-Oceanic (9%), and L2/East Asian (3%). The Euro-American lineage included more than six sublineages, the most abundant were: H (32%), T (23%), LAM (18%), and X (12%). 140 isolates (70%) were placed in 42 SITs patterns. Conclusions These results provide the first baseline data on the genetic structure of TB in the city of Veracruz. Sublineages H, X and LAM were predominant; however, it was founded an important diversity of genotypes that could contribute to the dispersion of TB and explain the high prevalence. This information might be useful for the development of further interventions to reduce impact of TB.
format	article
author	Roberto Zenteno-Cuevas Daniela Munro-Rojas Damián Pérez-Martínez Esdras Fernandez-Morales Ana C. Jimenez-Ruano Hilda Montero Leila Escobar Everest de Igartua Ángel Trigos Javier Fuentes-Dominguez
author_facet	Roberto Zenteno-Cuevas Daniela Munro-Rojas Damián Pérez-Martínez Esdras Fernandez-Morales Ana C. Jimenez-Ruano Hilda Montero Leila Escobar Everest de Igartua Ángel Trigos Javier Fuentes-Dominguez
author_sort	Roberto Zenteno-Cuevas
title	Genetic diversity and drug susceptibility of Mycobacterium tuberculosis in a city with a high prevalence of drug resistant tuberculosis from Southeast of Mexico
title_short	Genetic diversity and drug susceptibility of Mycobacterium tuberculosis in a city with a high prevalence of drug resistant tuberculosis from Southeast of Mexico
title_full	Genetic diversity and drug susceptibility of Mycobacterium tuberculosis in a city with a high prevalence of drug resistant tuberculosis from Southeast of Mexico
title_fullStr	Genetic diversity and drug susceptibility of Mycobacterium tuberculosis in a city with a high prevalence of drug resistant tuberculosis from Southeast of Mexico
title_full_unstemmed	Genetic diversity and drug susceptibility of Mycobacterium tuberculosis in a city with a high prevalence of drug resistant tuberculosis from Southeast of Mexico
title_sort	genetic diversity and drug susceptibility of mycobacterium tuberculosis in a city with a high prevalence of drug resistant tuberculosis from southeast of mexico
publisher	BMC
publishDate	2021
url	https://doaj.org/article/e30d0e01d06c497f99ce8e7345497e35
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Genetic diversity and drug susceptibility of Mycobacterium tuberculosis in a city with a high prevalence of drug resistant tuberculosis from Southeast of Mexico

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