Canonical Wnt/β-catenin signalling is essential for optic cup formation.

A multitude of signalling pathways are involved in the process of forming an eye. Here we demonstrate that β-catenin is essential for eye development as inactivation of β-catenin prior to cellular specification in the optic vesicle caused anophthalmia in mice. By achieving this early and tissue-spec...

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Autores principales:	Anna-Carin Hägglund, Anna Berghard, Leif Carlsson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e30e901822c042888d2b5bdf82aa986f
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spelling	oai:doaj.org-article:e30e901822c042888d2b5bdf82aa986f2021-11-18T08:43:34ZCanonical Wnt/β-catenin signalling is essential for optic cup formation.1932-620310.1371/journal.pone.0081158https://doaj.org/article/e30e901822c042888d2b5bdf82aa986f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24324671/?tool=EBIhttps://doaj.org/toc/1932-6203A multitude of signalling pathways are involved in the process of forming an eye. Here we demonstrate that β-catenin is essential for eye development as inactivation of β-catenin prior to cellular specification in the optic vesicle caused anophthalmia in mice. By achieving this early and tissue-specific β-catenin inactivation we find that retinal pigment epithelium (RPE) commitment was blocked and eye development was arrested prior to optic cup formation due to a loss of canonical Wnt signalling in the dorsal optic vesicle. Thus, these results show that Wnt/β-catenin signalling is required earlier and play a more central role in eye development than previous studies have indicated. In our genetic model system a few RPE cells could escape β-catenin inactivation leading to the formation of a small optic rudiment. The optic rudiment contained several neural retinal cell classes surrounded by an RPE. Unlike the RPE cells, the neural retinal cells could be β-catenin-negative revealing that differentiation of the neural retinal cell classes is β-catenin-independent. Moreover, although dorsoventral patterning is initiated in the mutant optic vesicle, the neural retinal cells in the optic rudiment displayed almost exclusively ventral identity. Thus, β-catenin is required for optic cup formation, commitment to RPE cells and maintenance of dorsal identity of the retina.Anna-Carin HägglundAnna BerghardLeif CarlssonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e81158 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna-Carin Hägglund Anna Berghard Leif Carlsson Canonical Wnt/β-catenin signalling is essential for optic cup formation.
description	A multitude of signalling pathways are involved in the process of forming an eye. Here we demonstrate that β-catenin is essential for eye development as inactivation of β-catenin prior to cellular specification in the optic vesicle caused anophthalmia in mice. By achieving this early and tissue-specific β-catenin inactivation we find that retinal pigment epithelium (RPE) commitment was blocked and eye development was arrested prior to optic cup formation due to a loss of canonical Wnt signalling in the dorsal optic vesicle. Thus, these results show that Wnt/β-catenin signalling is required earlier and play a more central role in eye development than previous studies have indicated. In our genetic model system a few RPE cells could escape β-catenin inactivation leading to the formation of a small optic rudiment. The optic rudiment contained several neural retinal cell classes surrounded by an RPE. Unlike the RPE cells, the neural retinal cells could be β-catenin-negative revealing that differentiation of the neural retinal cell classes is β-catenin-independent. Moreover, although dorsoventral patterning is initiated in the mutant optic vesicle, the neural retinal cells in the optic rudiment displayed almost exclusively ventral identity. Thus, β-catenin is required for optic cup formation, commitment to RPE cells and maintenance of dorsal identity of the retina.
format	article
author	Anna-Carin Hägglund Anna Berghard Leif Carlsson
author_facet	Anna-Carin Hägglund Anna Berghard Leif Carlsson
author_sort	Anna-Carin Hägglund
title	Canonical Wnt/β-catenin signalling is essential for optic cup formation.
title_short	Canonical Wnt/β-catenin signalling is essential for optic cup formation.
title_full	Canonical Wnt/β-catenin signalling is essential for optic cup formation.
title_fullStr	Canonical Wnt/β-catenin signalling is essential for optic cup formation.
title_full_unstemmed	Canonical Wnt/β-catenin signalling is essential for optic cup formation.
title_sort	canonical wnt/β-catenin signalling is essential for optic cup formation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e30e901822c042888d2b5bdf82aa986f
work_keys_str_mv	AT annacarinhagglund canonicalwntbcateninsignallingisessentialforopticcupformation AT annaberghard canonicalwntbcateninsignallingisessentialforopticcupformation AT leifcarlsson canonicalwntbcateninsignallingisessentialforopticcupformation
_version_	1718421414670761984

Canonical Wnt/β-catenin signalling is essential for optic cup formation.

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