The endogenous mex-3 3´UTR is required for germline repression and contributes to optimal fecundity in C. elegans.

RNA regulation is essential to successful reproduction. Messenger RNAs delivered from parent to progeny govern early embryonic development. RNA-binding proteins (RBPs) are the key effectors of this process, regulating the translation and stability of parental transcripts to control cell fate specifi...

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Autores principales:	Mennatallah M Y Albarqi, Sean P Ryder
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/e316d625d1bb47fe9cae6f5a7712138e
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spelling	oai:doaj.org-article:e316d625d1bb47fe9cae6f5a7712138e2021-12-02T20:02:51ZThe endogenous mex-3 3´UTR is required for germline repression and contributes to optimal fecundity in C. elegans.1553-73901553-740410.1371/journal.pgen.1009775https://doaj.org/article/e316d625d1bb47fe9cae6f5a7712138e2021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009775https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404RNA regulation is essential to successful reproduction. Messenger RNAs delivered from parent to progeny govern early embryonic development. RNA-binding proteins (RBPs) are the key effectors of this process, regulating the translation and stability of parental transcripts to control cell fate specification events prior to zygotic gene activation. The KH-domain RBP MEX-3 is conserved from nematode to human. It was first discovered in Caenorhabditis elegans, where it is essential for anterior cell fate and embryo viability. Here, we show that loss of the endogenous mex-3 3´UTR disrupts its germline expression pattern. An allelic series of 3´UTR deletion variants identify repressing regions of the UTR and demonstrate that repression is not precisely coupled to reproductive success. We also show that several RBPs regulate mex-3 mRNA through its 3´UTR to define its unique germline spatiotemporal expression pattern. Additionally, we find that both poly(A) tail length control and the translation initiation factor IFE-3 contribute to its expression pattern. Together, our results establish the importance of the mex-3 3´UTR to reproductive health and its expression in the germline. Our results suggest that additional mechanisms control MEX-3 function when 3´UTR regulation is compromised.Mennatallah M Y AlbarqiSean P RyderPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 8, p e1009775 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Mennatallah M Y Albarqi Sean P Ryder The endogenous mex-3 3´UTR is required for germline repression and contributes to optimal fecundity in C. elegans.
description	RNA regulation is essential to successful reproduction. Messenger RNAs delivered from parent to progeny govern early embryonic development. RNA-binding proteins (RBPs) are the key effectors of this process, regulating the translation and stability of parental transcripts to control cell fate specification events prior to zygotic gene activation. The KH-domain RBP MEX-3 is conserved from nematode to human. It was first discovered in Caenorhabditis elegans, where it is essential for anterior cell fate and embryo viability. Here, we show that loss of the endogenous mex-3 3´UTR disrupts its germline expression pattern. An allelic series of 3´UTR deletion variants identify repressing regions of the UTR and demonstrate that repression is not precisely coupled to reproductive success. We also show that several RBPs regulate mex-3 mRNA through its 3´UTR to define its unique germline spatiotemporal expression pattern. Additionally, we find that both poly(A) tail length control and the translation initiation factor IFE-3 contribute to its expression pattern. Together, our results establish the importance of the mex-3 3´UTR to reproductive health and its expression in the germline. Our results suggest that additional mechanisms control MEX-3 function when 3´UTR regulation is compromised.
format	article
author	Mennatallah M Y Albarqi Sean P Ryder
author_facet	Mennatallah M Y Albarqi Sean P Ryder
author_sort	Mennatallah M Y Albarqi
title	The endogenous mex-3 3´UTR is required for germline repression and contributes to optimal fecundity in C. elegans.
title_short	The endogenous mex-3 3´UTR is required for germline repression and contributes to optimal fecundity in C. elegans.
title_full	The endogenous mex-3 3´UTR is required for germline repression and contributes to optimal fecundity in C. elegans.
title_fullStr	The endogenous mex-3 3´UTR is required for germline repression and contributes to optimal fecundity in C. elegans.
title_full_unstemmed	The endogenous mex-3 3´UTR is required for germline repression and contributes to optimal fecundity in C. elegans.
title_sort	endogenous mex-3 3´utr is required for germline repression and contributes to optimal fecundity in c. elegans.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/e316d625d1bb47fe9cae6f5a7712138e
work_keys_str_mv	AT mennatallahmyalbarqi theendogenousmex33utrisrequiredforgermlinerepressionandcontributestooptimalfecundityincelegans AT seanpryder theendogenousmex33utrisrequiredforgermlinerepressionandcontributestooptimalfecundityincelegans AT mennatallahmyalbarqi endogenousmex33utrisrequiredforgermlinerepressionandcontributestooptimalfecundityincelegans AT seanpryder endogenousmex33utrisrequiredforgermlinerepressionandcontributestooptimalfecundityincelegans
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The endogenous mex-3 3´UTR is required for germline repression and contributes to optimal fecundity in C. elegans.

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