SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation

Type III CRISPR-Cas systems recognize and cleave target RNAs and produce signalling molecules. Here the authors discover that both processes are governed by a flexible seed region, ultimately resulting in SCOPE, a SARSCoV-2 diagnostic assay with atto-molar sensitivity.

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Autores principales:	Jurre A. Steens, Yifan Zhu, David W. Taylor, Jack P. K. Bravo, Stijn H. P. Prinsen, Cor D. Schoen, Bart J. F. Keijser, Michel Ossendrijver, L. Marije Hofstra, Stan J. J. Brouns, Akeo Shinkai, John van der Oost, Raymond H. J. Staals
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e3174dc9798f445a945c96d9e82fdcc7
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spelling	oai:doaj.org-article:e3174dc9798f445a945c96d9e82fdcc72021-12-02T16:46:36ZSCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation10.1038/s41467-021-25337-52041-1723https://doaj.org/article/e3174dc9798f445a945c96d9e82fdcc72021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25337-5https://doaj.org/toc/2041-1723Type III CRISPR-Cas systems recognize and cleave target RNAs and produce signalling molecules. Here the authors discover that both processes are governed by a flexible seed region, ultimately resulting in SCOPE, a SARSCoV-2 diagnostic assay with atto-molar sensitivity.Jurre A. SteensYifan ZhuDavid W. TaylorJack P. K. BravoStijn H. P. PrinsenCor D. SchoenBart J. F. KeijserMichel OssendrijverL. Marije HofstraStan J. J. BrounsAkeo ShinkaiJohn van der OostRaymond H. J. StaalsNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jurre A. Steens Yifan Zhu David W. Taylor Jack P. K. Bravo Stijn H. P. Prinsen Cor D. Schoen Bart J. F. Keijser Michel Ossendrijver L. Marije Hofstra Stan J. J. Brouns Akeo Shinkai John van der Oost Raymond H. J. Staals SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation
description	Type III CRISPR-Cas systems recognize and cleave target RNAs and produce signalling molecules. Here the authors discover that both processes are governed by a flexible seed region, ultimately resulting in SCOPE, a SARSCoV-2 diagnostic assay with atto-molar sensitivity.
format	article
author	Jurre A. Steens Yifan Zhu David W. Taylor Jack P. K. Bravo Stijn H. P. Prinsen Cor D. Schoen Bart J. F. Keijser Michel Ossendrijver L. Marije Hofstra Stan J. J. Brouns Akeo Shinkai John van der Oost Raymond H. J. Staals
author_facet	Jurre A. Steens Yifan Zhu David W. Taylor Jack P. K. Bravo Stijn H. P. Prinsen Cor D. Schoen Bart J. F. Keijser Michel Ossendrijver L. Marije Hofstra Stan J. J. Brouns Akeo Shinkai John van der Oost Raymond H. J. Staals
author_sort	Jurre A. Steens
title	SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation
title_short	SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation
title_full	SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation
title_fullStr	SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation
title_full_unstemmed	SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation
title_sort	scope enables type iii crispr-cas diagnostics using flexible targeting and stringent carf ribonuclease activation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e3174dc9798f445a945c96d9e82fdcc7
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SCOPE enables type III CRISPR-Cas diagnostics using flexible targeting and stringent CARF ribonuclease activation

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