Evaluation of the neuroprotective and antioxidant effects of Dorema aucheri extract on cerebral ischaemia-reperfusion injury in rats

Context: The hydroalcoholic extract of Dorema aucheri Bilhar (Umbelliferae) (DA) leaves, a medicinal plant, has powerful antioxidant properties. Objective: This study evaluates the neuroprotective effects of pre-treatment with DA leaves extract against cerebral ischaemia-induced brain injury through...

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Autores principales:	Javad Rasouli Vani, Mohammad Taghi Mohammadi, Mahsa Sarami Foroshani, Elham Rezazade
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	brain infarction brain swelling oxidative stress nitrosative stress Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/e32d832c9a8f4f0d961baee287613032
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spelling	oai:doaj.org-article:e32d832c9a8f4f0d961baee2876130322021-11-17T14:21:56ZEvaluation of the neuroprotective and antioxidant effects of Dorema aucheri extract on cerebral ischaemia-reperfusion injury in rats1388-02091744-511610.1080/13880209.2019.1597132https://doaj.org/article/e32d832c9a8f4f0d961baee2876130322019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1597132https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: The hydroalcoholic extract of Dorema aucheri Bilhar (Umbelliferae) (DA) leaves, a medicinal plant, has powerful antioxidant properties. Objective: This study evaluates the neuroprotective effects of pre-treatment with DA leaves extract against cerebral ischaemia-induced brain injury through alteration of the antioxidant capacity. Materials and methods: The study was conducted in three groups of Wistar rats (N = 47) as follows; sham, control ischaemic and pre-treated ischaemic groups. Rats were administered a fresh hydroalcoholic extract of DA leaves at a dosage of 200 mg/kg/day for 14 days. Then, the middle cerebral artery (MCA) of the right hemisphere was occluded for 90 min to achieve cerebral ischaemia. After 24 h reperfusion, cerebral infarction and superoxide dismutase (SOD) and catalase activities, as well as malondialdehyde (MDA), glutathione, and NOx contents were determined in the right hemispheres. Results: Occlusion of the right MCA caused noticeable cerebral infarction (298 ± 21 mm3) in control ischaemic group, but pre-treatment with DA extract considerably attenuated it (92 ± 14 mm3) in the pre-treated ischaemic group. DA extract significantly decreased the levels of MDA by 28% and NOx by 11% in pre-treated ischaemic group compared to the control ischaemic group. DA extract also enhanced glutathione content by 7%, SOD activity by 16% and catalase activity by 46% in pre-treated ischaemic rats compared to control ischaemic rats. Discussion and conclusions: DA is able to improve the antioxidant capacity and injuries of ischaemic brain. It is proposed as a neuroprotectant following cerebral ischaemia to decrease the injuries of ischaemic stroke.Javad Rasouli VaniMohammad Taghi MohammadiMahsa Sarami ForoshaniElham RezazadeTaylor & Francis Grouparticlebrain infarctionbrain swellingoxidative stressnitrosative stressTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 255-262 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	brain infarction brain swelling oxidative stress nitrosative stress Therapeutics. Pharmacology RM1-950
spellingShingle	brain infarction brain swelling oxidative stress nitrosative stress Therapeutics. Pharmacology RM1-950 Javad Rasouli Vani Mohammad Taghi Mohammadi Mahsa Sarami Foroshani Elham Rezazade Evaluation of the neuroprotective and antioxidant effects of Dorema aucheri extract on cerebral ischaemia-reperfusion injury in rats
description	Context: The hydroalcoholic extract of Dorema aucheri Bilhar (Umbelliferae) (DA) leaves, a medicinal plant, has powerful antioxidant properties. Objective: This study evaluates the neuroprotective effects of pre-treatment with DA leaves extract against cerebral ischaemia-induced brain injury through alteration of the antioxidant capacity. Materials and methods: The study was conducted in three groups of Wistar rats (N = 47) as follows; sham, control ischaemic and pre-treated ischaemic groups. Rats were administered a fresh hydroalcoholic extract of DA leaves at a dosage of 200 mg/kg/day for 14 days. Then, the middle cerebral artery (MCA) of the right hemisphere was occluded for 90 min to achieve cerebral ischaemia. After 24 h reperfusion, cerebral infarction and superoxide dismutase (SOD) and catalase activities, as well as malondialdehyde (MDA), glutathione, and NOx contents were determined in the right hemispheres. Results: Occlusion of the right MCA caused noticeable cerebral infarction (298 ± 21 mm3) in control ischaemic group, but pre-treatment with DA extract considerably attenuated it (92 ± 14 mm3) in the pre-treated ischaemic group. DA extract significantly decreased the levels of MDA by 28% and NOx by 11% in pre-treated ischaemic group compared to the control ischaemic group. DA extract also enhanced glutathione content by 7%, SOD activity by 16% and catalase activity by 46% in pre-treated ischaemic rats compared to control ischaemic rats. Discussion and conclusions: DA is able to improve the antioxidant capacity and injuries of ischaemic brain. It is proposed as a neuroprotectant following cerebral ischaemia to decrease the injuries of ischaemic stroke.
format	article
author	Javad Rasouli Vani Mohammad Taghi Mohammadi Mahsa Sarami Foroshani Elham Rezazade
author_facet	Javad Rasouli Vani Mohammad Taghi Mohammadi Mahsa Sarami Foroshani Elham Rezazade
author_sort	Javad Rasouli Vani
title	Evaluation of the neuroprotective and antioxidant effects of Dorema aucheri extract on cerebral ischaemia-reperfusion injury in rats
title_short	Evaluation of the neuroprotective and antioxidant effects of Dorema aucheri extract on cerebral ischaemia-reperfusion injury in rats
title_full	Evaluation of the neuroprotective and antioxidant effects of Dorema aucheri extract on cerebral ischaemia-reperfusion injury in rats
title_fullStr	Evaluation of the neuroprotective and antioxidant effects of Dorema aucheri extract on cerebral ischaemia-reperfusion injury in rats
title_full_unstemmed	Evaluation of the neuroprotective and antioxidant effects of Dorema aucheri extract on cerebral ischaemia-reperfusion injury in rats
title_sort	evaluation of the neuroprotective and antioxidant effects of dorema aucheri extract on cerebral ischaemia-reperfusion injury in rats
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/e32d832c9a8f4f0d961baee287613032
work_keys_str_mv	AT javadrasoulivani evaluationoftheneuroprotectiveandantioxidanteffectsofdoremaaucheriextractoncerebralischaemiareperfusioninjuryinrats AT mohammadtaghimohammadi evaluationoftheneuroprotectiveandantioxidanteffectsofdoremaaucheriextractoncerebralischaemiareperfusioninjuryinrats AT mahsasaramiforoshani evaluationoftheneuroprotectiveandantioxidanteffectsofdoremaaucheriextractoncerebralischaemiareperfusioninjuryinrats AT elhamrezazade evaluationoftheneuroprotectiveandantioxidanteffectsofdoremaaucheriextractoncerebralischaemiareperfusioninjuryinrats
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Evaluation of the neuroprotective and antioxidant effects of Dorema aucheri extract on cerebral ischaemia-reperfusion injury in rats

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