Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling

Abstract Soil is considered an extensively explored ecological niche for microorganisms that produce useful biologically active natural products suitable for pharmaceutical applications. The current study aimed at investigating biological activities and metabolic profiles of three fungal strains ide...

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Autores principales:	Afrah E. Mohammed, Hana Sonbol, Suaad Saleh Alwakeel, Modhi O. Alotaibi, Sohailah Alotaibi, Nouf Alothman, Rasha Saad Suliman, Hanadi Talal Ahmedah, Rizwan Ali
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e3356b2f041f437c80fe1821f253a21d
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spelling	oai:doaj.org-article:e3356b2f041f437c80fe1821f253a21d2021-12-02T15:52:59ZInvestigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling10.1038/s41598-021-83556-82045-2322https://doaj.org/article/e3356b2f041f437c80fe1821f253a21d2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83556-8https://doaj.org/toc/2045-2322Abstract Soil is considered an extensively explored ecological niche for microorganisms that produce useful biologically active natural products suitable for pharmaceutical applications. The current study aimed at investigating biological activities and metabolic profiles of three fungal strains identified from different desert sites in Saudi Arabia. Soil fungal isolates were collected from AlQasab, Tabuk, and Almuzahimiyah in Saudi Arabia and identified. Furthermore, their antibacterial activity was investigated against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumonia, and Escherichia coli in blood, nutrient, and Sabouraud dextrose agars. Moreover, fungal extracts were evaluated on cell viability/proliferation against human breast carcinoma and colorectal adenocarcinoma cells. To identify the biomolecules of the fungal extracts, High-performance liquid chromatography HPLC–DAD coupled to analytical LC–QTOF-MS method was employed for fungal ethyl acetate crude extract. Identified fungal isolates, Chaetomium sp. Bipolaris sp. and Fusarium venenatum showed varied inhibitory activity against tested microbes in relation to crude extract, microbial strain tested, and growth media. F. venenatum showed higher anticancer activity compared to Chaetomium sp. and Bipolaris sp. extracts against four of the tested cancer cell lines. Screening by HPLC and LC/MS-QTOF identified nine compounds from Chaetomium sp. and three from Bipolaris sp. however, for F. venenatum extracts compounds were not fully identified. In light of the present findings, some biological activities of fungal extracts were approved in vitro, suggesting that such extracts could be a useful starting point to find compounds that possess promising agents for medical applications. Further investigations to identify exact biomolecules from F. venenatum extracts are needed.Afrah E. MohammedHana SonbolSuaad Saleh AlwakeelModhi O. AlotaibiSohailah AlotaibiNouf AlothmanRasha Saad SulimanHanadi Talal AhmedahRizwan AliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Afrah E. Mohammed Hana Sonbol Suaad Saleh Alwakeel Modhi O. Alotaibi Sohailah Alotaibi Nouf Alothman Rasha Saad Suliman Hanadi Talal Ahmedah Rizwan Ali Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling
description	Abstract Soil is considered an extensively explored ecological niche for microorganisms that produce useful biologically active natural products suitable for pharmaceutical applications. The current study aimed at investigating biological activities and metabolic profiles of three fungal strains identified from different desert sites in Saudi Arabia. Soil fungal isolates were collected from AlQasab, Tabuk, and Almuzahimiyah in Saudi Arabia and identified. Furthermore, their antibacterial activity was investigated against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumonia, and Escherichia coli in blood, nutrient, and Sabouraud dextrose agars. Moreover, fungal extracts were evaluated on cell viability/proliferation against human breast carcinoma and colorectal adenocarcinoma cells. To identify the biomolecules of the fungal extracts, High-performance liquid chromatography HPLC–DAD coupled to analytical LC–QTOF-MS method was employed for fungal ethyl acetate crude extract. Identified fungal isolates, Chaetomium sp. Bipolaris sp. and Fusarium venenatum showed varied inhibitory activity against tested microbes in relation to crude extract, microbial strain tested, and growth media. F. venenatum showed higher anticancer activity compared to Chaetomium sp. and Bipolaris sp. extracts against four of the tested cancer cell lines. Screening by HPLC and LC/MS-QTOF identified nine compounds from Chaetomium sp. and three from Bipolaris sp. however, for F. venenatum extracts compounds were not fully identified. In light of the present findings, some biological activities of fungal extracts were approved in vitro, suggesting that such extracts could be a useful starting point to find compounds that possess promising agents for medical applications. Further investigations to identify exact biomolecules from F. venenatum extracts are needed.
format	article
author	Afrah E. Mohammed Hana Sonbol Suaad Saleh Alwakeel Modhi O. Alotaibi Sohailah Alotaibi Nouf Alothman Rasha Saad Suliman Hanadi Talal Ahmedah Rizwan Ali
author_facet	Afrah E. Mohammed Hana Sonbol Suaad Saleh Alwakeel Modhi O. Alotaibi Sohailah Alotaibi Nouf Alothman Rasha Saad Suliman Hanadi Talal Ahmedah Rizwan Ali
author_sort	Afrah E. Mohammed
title	Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling
title_short	Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling
title_full	Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling
title_fullStr	Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling
title_full_unstemmed	Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling
title_sort	investigation of biological activity of soil fungal extracts and lc/ms-qtof based metabolite profiling
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e3356b2f041f437c80fe1821f253a21d
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Investigation of biological activity of soil fungal extracts and LC/MS-QTOF based metabolite profiling

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