Partner independent fusion gene detection by multiplexed CRISPR-Cas9 enrichment and long read nanopore sequencing

Fusion genes are a hallmarks of cancer, though breakpoint-position promiscuity restricts diagnostic detection. Here, the authors present FUDGE, a CRISPR-Cas9-based enrichment strategy for nanopore sequencing to identify target fusions irrespective of genomic breakpoint or fusion partner.

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Detalles Bibliográficos
Autores principales:	Christina Stangl, Sam de Blank, Ivo Renkens, Liset Westera, Tamara Verbeek, Jose Espejo Valle-Inclan, Rocio Chamorro González, Anton G. Henssen, Markus J. van Roosmalen, Ronald W. Stam, Emile E. Voest, Wigard P. Kloosterman, Gijs van Haaften, Glen R. Monroe
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e33d69e505f647c4a248cf888947cfbb
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spelling	oai:doaj.org-article:e33d69e505f647c4a248cf888947cfbb2021-12-02T15:57:18ZPartner independent fusion gene detection by multiplexed CRISPR-Cas9 enrichment and long read nanopore sequencing10.1038/s41467-020-16641-72041-1723https://doaj.org/article/e33d69e505f647c4a248cf888947cfbb2020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16641-7https://doaj.org/toc/2041-1723Fusion genes are a hallmarks of cancer, though breakpoint-position promiscuity restricts diagnostic detection. Here, the authors present FUDGE, a CRISPR-Cas9-based enrichment strategy for nanopore sequencing to identify target fusions irrespective of genomic breakpoint or fusion partner.Christina StanglSam de BlankIvo RenkensLiset WesteraTamara VerbeekJose Espejo Valle-InclanRocio Chamorro GonzálezAnton G. HenssenMarkus J. van RoosmalenRonald W. StamEmile E. VoestWigard P. KloostermanGijs van HaaftenGlen R. MonroeNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Christina Stangl Sam de Blank Ivo Renkens Liset Westera Tamara Verbeek Jose Espejo Valle-Inclan Rocio Chamorro González Anton G. Henssen Markus J. van Roosmalen Ronald W. Stam Emile E. Voest Wigard P. Kloosterman Gijs van Haaften Glen R. Monroe Partner independent fusion gene detection by multiplexed CRISPR-Cas9 enrichment and long read nanopore sequencing
description	Fusion genes are a hallmarks of cancer, though breakpoint-position promiscuity restricts diagnostic detection. Here, the authors present FUDGE, a CRISPR-Cas9-based enrichment strategy for nanopore sequencing to identify target fusions irrespective of genomic breakpoint or fusion partner.
format	article
author	Christina Stangl Sam de Blank Ivo Renkens Liset Westera Tamara Verbeek Jose Espejo Valle-Inclan Rocio Chamorro González Anton G. Henssen Markus J. van Roosmalen Ronald W. Stam Emile E. Voest Wigard P. Kloosterman Gijs van Haaften Glen R. Monroe
author_facet	Christina Stangl Sam de Blank Ivo Renkens Liset Westera Tamara Verbeek Jose Espejo Valle-Inclan Rocio Chamorro González Anton G. Henssen Markus J. van Roosmalen Ronald W. Stam Emile E. Voest Wigard P. Kloosterman Gijs van Haaften Glen R. Monroe
author_sort	Christina Stangl
title	Partner independent fusion gene detection by multiplexed CRISPR-Cas9 enrichment and long read nanopore sequencing
title_short	Partner independent fusion gene detection by multiplexed CRISPR-Cas9 enrichment and long read nanopore sequencing
title_full	Partner independent fusion gene detection by multiplexed CRISPR-Cas9 enrichment and long read nanopore sequencing
title_fullStr	Partner independent fusion gene detection by multiplexed CRISPR-Cas9 enrichment and long read nanopore sequencing
title_full_unstemmed	Partner independent fusion gene detection by multiplexed CRISPR-Cas9 enrichment and long read nanopore sequencing
title_sort	partner independent fusion gene detection by multiplexed crispr-cas9 enrichment and long read nanopore sequencing
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/e33d69e505f647c4a248cf888947cfbb
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Partner independent fusion gene detection by multiplexed CRISPR-Cas9 enrichment and long read nanopore sequencing

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