Cooperation of Adhesin Alleles in <italic toggle="yes">Salmonella</italic>-Host Tropism

ABSTRACT Allelic combinations and host specificities for three fimbrial adhesins, FimH, BcfD, and StfH, were compared for 262 strains of Salmonella enterica serovar Newport, a frequent human and livestock pathogen. Like FimH, BcfD had two major alleles (designated A and B), whereas StfH had two alle...

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Autores principales:	Leon De Masi, Min Yue, Changmin Hu, Alexey V. Rakov, Shelley C. Rankin, Dieter M. Schifferli
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Salmonella Newport adhesins allelic variation fimbriae host tropism Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e3534f5bbee84192aa50ca4ed57e9d82
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spelling	oai:doaj.org-article:e3534f5bbee84192aa50ca4ed57e9d822021-11-15T15:21:46ZCooperation of Adhesin Alleles in <italic toggle="yes">Salmonella</italic>-Host Tropism10.1128/mSphere.00066-172379-5042https://doaj.org/article/e3534f5bbee84192aa50ca4ed57e9d822017-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00066-17https://doaj.org/toc/2379-5042ABSTRACT Allelic combinations and host specificities for three fimbrial adhesins, FimH, BcfD, and StfH, were compared for 262 strains of Salmonella enterica serovar Newport, a frequent human and livestock pathogen. Like FimH, BcfD had two major alleles (designated A and B), whereas StfH had two allelic groups, each with two alleles (subgroup A1 and A2 and subgroup B1 and B2). The most prevalent combinations of FimH/BcfD/StfH alleles in S. Newport were A/A/A1 and B/B/B1. The former set was most frequently found in bovine and porcine strains, whereas the latter combination was most frequently found in environmental and human isolates. Bacteria genetically engineered to express Fim, Bcf, or Stf fimbriae on their surface were tested with the different alleles for binding to human, porcine, and bovine intestinal epithelial cells. The major allelic combinations with bovine and porcine strains (A/A/A1) or with human isolates (B/B/B1) provided at least two alleles capable of binding significantly better than the other alleles to an intestinal epithelial cell line from the respective host(s). However, each combination of alleles kept at least one allele mediating binding to an intestinal epithelial cell from another host. These findings indicated that allelic variation in multiple adhesins of S. Newport contributes to bacterial adaptation to certain preferential hosts without losing the capacity to maintain a broad host range. IMPORTANCE Salmonella enterica remains a leading foodborne bacterial pathogen in the United States; infected livestock serve often as the source of contaminated food products. A study estimated that over a billion Salmonella gastroenteritis cases and up to 33 million typhoid cases occur annually worldwide, with 3.5 million deaths. Although many Salmonella strains with a broad host range present preferential associations with certain host species, it is not clear what determines the various levels of host adaptation. Here, causal properties of host associations were determined with allelic variants of three colonization factors of S. enterica serovar Newport, a most frequent zoonotic serovar. This is the first study that related not only individual but also a small group of host-associated gene variants with functional properties that cooperate to determine the level of host-adapted virulence. The detected associations should help to identify sources of Salmonella infections in both humans and animals.Leon De MasiMin YueChangmin HuAlexey V. RakovShelley C. RankinDieter M. SchifferliAmerican Society for MicrobiologyarticleSalmonella Newportadhesinsallelic variationfimbriaehost tropismMicrobiologyQR1-502ENmSphere, Vol 2, Iss 2 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Salmonella Newport adhesins allelic variation fimbriae host tropism Microbiology QR1-502
spellingShingle	Salmonella Newport adhesins allelic variation fimbriae host tropism Microbiology QR1-502 Leon De Masi Min Yue Changmin Hu Alexey V. Rakov Shelley C. Rankin Dieter M. Schifferli Cooperation of Adhesin Alleles in <italic toggle="yes">Salmonella</italic>-Host Tropism
description	ABSTRACT Allelic combinations and host specificities for three fimbrial adhesins, FimH, BcfD, and StfH, were compared for 262 strains of Salmonella enterica serovar Newport, a frequent human and livestock pathogen. Like FimH, BcfD had two major alleles (designated A and B), whereas StfH had two allelic groups, each with two alleles (subgroup A1 and A2 and subgroup B1 and B2). The most prevalent combinations of FimH/BcfD/StfH alleles in S. Newport were A/A/A1 and B/B/B1. The former set was most frequently found in bovine and porcine strains, whereas the latter combination was most frequently found in environmental and human isolates. Bacteria genetically engineered to express Fim, Bcf, or Stf fimbriae on their surface were tested with the different alleles for binding to human, porcine, and bovine intestinal epithelial cells. The major allelic combinations with bovine and porcine strains (A/A/A1) or with human isolates (B/B/B1) provided at least two alleles capable of binding significantly better than the other alleles to an intestinal epithelial cell line from the respective host(s). However, each combination of alleles kept at least one allele mediating binding to an intestinal epithelial cell from another host. These findings indicated that allelic variation in multiple adhesins of S. Newport contributes to bacterial adaptation to certain preferential hosts without losing the capacity to maintain a broad host range. IMPORTANCE Salmonella enterica remains a leading foodborne bacterial pathogen in the United States; infected livestock serve often as the source of contaminated food products. A study estimated that over a billion Salmonella gastroenteritis cases and up to 33 million typhoid cases occur annually worldwide, with 3.5 million deaths. Although many Salmonella strains with a broad host range present preferential associations with certain host species, it is not clear what determines the various levels of host adaptation. Here, causal properties of host associations were determined with allelic variants of three colonization factors of S. enterica serovar Newport, a most frequent zoonotic serovar. This is the first study that related not only individual but also a small group of host-associated gene variants with functional properties that cooperate to determine the level of host-adapted virulence. The detected associations should help to identify sources of Salmonella infections in both humans and animals.
format	article
author	Leon De Masi Min Yue Changmin Hu Alexey V. Rakov Shelley C. Rankin Dieter M. Schifferli
author_facet	Leon De Masi Min Yue Changmin Hu Alexey V. Rakov Shelley C. Rankin Dieter M. Schifferli
author_sort	Leon De Masi
title	Cooperation of Adhesin Alleles in <italic toggle="yes">Salmonella</italic>-Host Tropism
title_short	Cooperation of Adhesin Alleles in <italic toggle="yes">Salmonella</italic>-Host Tropism
title_full	Cooperation of Adhesin Alleles in <italic toggle="yes">Salmonella</italic>-Host Tropism
title_fullStr	Cooperation of Adhesin Alleles in <italic toggle="yes">Salmonella</italic>-Host Tropism
title_full_unstemmed	Cooperation of Adhesin Alleles in <italic toggle="yes">Salmonella</italic>-Host Tropism
title_sort	cooperation of adhesin alleles in <italic toggle="yes">salmonella</italic>-host tropism
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/e3534f5bbee84192aa50ca4ed57e9d82
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Cooperation of Adhesin Alleles in <italic toggle="yes">Salmonella</italic>-Host Tropism

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