Nasal microbiota clusters associate with inflammatory response, viral load, and symptom severity in experimental rhinovirus challenge

Abstract The role of nasal and fecal microbiota in viral respiratory infections has not been established. We collected nasal swabs and washes, and fecal samples in a clinical study assessing the effect of probiotic Bifidobacterium animalis subsp. lactis Bl-04 on experimental rhinovirus infection. Th...

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Autores principales:	Markus J. Lehtinen, Ashley A. Hibberd, Sofia Männikkö, Nicolas Yeung, Tommi Kauko, Sofia Forssten, Liisa Lehtoranta, Sampo J. Lahtinen, Buffy Stahl, Anna Lyra, Ronald B. Turner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e356adef849345e5961d55ed4fc753c9
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spelling	oai:doaj.org-article:e356adef849345e5961d55ed4fc753c92021-12-02T11:40:36ZNasal microbiota clusters associate with inflammatory response, viral load, and symptom severity in experimental rhinovirus challenge10.1038/s41598-018-29793-w2045-2322https://doaj.org/article/e356adef849345e5961d55ed4fc753c92018-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-29793-whttps://doaj.org/toc/2045-2322Abstract The role of nasal and fecal microbiota in viral respiratory infections has not been established. We collected nasal swabs and washes, and fecal samples in a clinical study assessing the effect of probiotic Bifidobacterium animalis subsp. lactis Bl-04 on experimental rhinovirus infection. The nasal and fecal microbiota were characterized by 16S rRNA gene sequencing. The resulting data were compared with nasal inflammatory marker concentrations, viral load, and clinical symptoms. By using unsupervised clustering, the nasal microbiota divided into six clusters. The clusters predominant of Staphylococcus, Corynebacterium/Alloiococcus, Moraxella, and Pseudomonadaceae/Mixed had characteristic inflammatory marker and viral load profiles in nasal washes. The nasal microbiota clusters of subjects before the infection associated with the severity of clinical cold symptoms during rhinovirus infection. Rhinovirus infection and probiotic intervention did not significantly alter the composition of nasal or fecal microbiota. Our results suggest that nasal microbiota may influence the virus load, host innate immune response, and clinical symptoms during rhinovirus infection, however, further studies are needed.Markus J. LehtinenAshley A. HibberdSofia MännikköNicolas YeungTommi KaukoSofia ForsstenLiisa LehtorantaSampo J. LahtinenBuffy StahlAnna LyraRonald B. TurnerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Markus J. Lehtinen Ashley A. Hibberd Sofia Männikkö Nicolas Yeung Tommi Kauko Sofia Forssten Liisa Lehtoranta Sampo J. Lahtinen Buffy Stahl Anna Lyra Ronald B. Turner Nasal microbiota clusters associate with inflammatory response, viral load, and symptom severity in experimental rhinovirus challenge
description	Abstract The role of nasal and fecal microbiota in viral respiratory infections has not been established. We collected nasal swabs and washes, and fecal samples in a clinical study assessing the effect of probiotic Bifidobacterium animalis subsp. lactis Bl-04 on experimental rhinovirus infection. The nasal and fecal microbiota were characterized by 16S rRNA gene sequencing. The resulting data were compared with nasal inflammatory marker concentrations, viral load, and clinical symptoms. By using unsupervised clustering, the nasal microbiota divided into six clusters. The clusters predominant of Staphylococcus, Corynebacterium/Alloiococcus, Moraxella, and Pseudomonadaceae/Mixed had characteristic inflammatory marker and viral load profiles in nasal washes. The nasal microbiota clusters of subjects before the infection associated with the severity of clinical cold symptoms during rhinovirus infection. Rhinovirus infection and probiotic intervention did not significantly alter the composition of nasal or fecal microbiota. Our results suggest that nasal microbiota may influence the virus load, host innate immune response, and clinical symptoms during rhinovirus infection, however, further studies are needed.
format	article
author	Markus J. Lehtinen Ashley A. Hibberd Sofia Männikkö Nicolas Yeung Tommi Kauko Sofia Forssten Liisa Lehtoranta Sampo J. Lahtinen Buffy Stahl Anna Lyra Ronald B. Turner
author_facet	Markus J. Lehtinen Ashley A. Hibberd Sofia Männikkö Nicolas Yeung Tommi Kauko Sofia Forssten Liisa Lehtoranta Sampo J. Lahtinen Buffy Stahl Anna Lyra Ronald B. Turner
author_sort	Markus J. Lehtinen
title	Nasal microbiota clusters associate with inflammatory response, viral load, and symptom severity in experimental rhinovirus challenge
title_short	Nasal microbiota clusters associate with inflammatory response, viral load, and symptom severity in experimental rhinovirus challenge
title_full	Nasal microbiota clusters associate with inflammatory response, viral load, and symptom severity in experimental rhinovirus challenge
title_fullStr	Nasal microbiota clusters associate with inflammatory response, viral load, and symptom severity in experimental rhinovirus challenge
title_full_unstemmed	Nasal microbiota clusters associate with inflammatory response, viral load, and symptom severity in experimental rhinovirus challenge
title_sort	nasal microbiota clusters associate with inflammatory response, viral load, and symptom severity in experimental rhinovirus challenge
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/e356adef849345e5961d55ed4fc753c9
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Nasal microbiota clusters associate with inflammatory response, viral load, and symptom severity in experimental rhinovirus challenge

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