Differential expression profile of gluten-specific T cells identified by single-cell RNA-seq.

Gluten-specific CD4+ T cells drive the pathogenesis of celiac disease and circulating gluten-specific T cells can be identified by staining with HLA-DQ:gluten tetramers. In this first single-cell RNA-seq study of tetramer-sorted T cells from untreated celiac disease patients blood, we found that glu...

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Autores principales:	Ying Yao, Łukasz Wyrozżemski, Knut E A Lundin, Geir Kjetil Sandve, Shuo-Wang Qiao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e35c933146b7496389afe831b83ade44
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spelling	oai:doaj.org-article:e35c933146b7496389afe831b83ade442021-12-02T20:17:12ZDifferential expression profile of gluten-specific T cells identified by single-cell RNA-seq.1932-620310.1371/journal.pone.0258029https://doaj.org/article/e35c933146b7496389afe831b83ade442021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258029https://doaj.org/toc/1932-6203Gluten-specific CD4+ T cells drive the pathogenesis of celiac disease and circulating gluten-specific T cells can be identified by staining with HLA-DQ:gluten tetramers. In this first single-cell RNA-seq study of tetramer-sorted T cells from untreated celiac disease patients blood, we found that gluten-specific T cells showed distinct transcriptomic profiles consistent with activated effector memory T cells that shared features with Th1 and follicular helper T cells. Compared to non-specific cells, gluten-specific T cells showed differential expression of several genes involved in T-cell receptor signaling, translational processes, apoptosis, fatty acid transport, and redox potentials. Many of the gluten-specific T cells studied shared T-cell receptor with each other, indicating that circulating gluten-specific T cells belong to a limited number of clones. Moreover, the transcriptional profiles of cells that shared the same clonal origin were transcriptionally more similar compared with between clonally unrelated gluten-specific cells.Ying YaoŁukasz WyrozżemskiKnut E A LundinGeir Kjetil SandveShuo-Wang QiaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0258029 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ying Yao Łukasz Wyrozżemski Knut E A Lundin Geir Kjetil Sandve Shuo-Wang Qiao Differential expression profile of gluten-specific T cells identified by single-cell RNA-seq.
description	Gluten-specific CD4+ T cells drive the pathogenesis of celiac disease and circulating gluten-specific T cells can be identified by staining with HLA-DQ:gluten tetramers. In this first single-cell RNA-seq study of tetramer-sorted T cells from untreated celiac disease patients blood, we found that gluten-specific T cells showed distinct transcriptomic profiles consistent with activated effector memory T cells that shared features with Th1 and follicular helper T cells. Compared to non-specific cells, gluten-specific T cells showed differential expression of several genes involved in T-cell receptor signaling, translational processes, apoptosis, fatty acid transport, and redox potentials. Many of the gluten-specific T cells studied shared T-cell receptor with each other, indicating that circulating gluten-specific T cells belong to a limited number of clones. Moreover, the transcriptional profiles of cells that shared the same clonal origin were transcriptionally more similar compared with between clonally unrelated gluten-specific cells.
format	article
author	Ying Yao Łukasz Wyrozżemski Knut E A Lundin Geir Kjetil Sandve Shuo-Wang Qiao
author_facet	Ying Yao Łukasz Wyrozżemski Knut E A Lundin Geir Kjetil Sandve Shuo-Wang Qiao
author_sort	Ying Yao
title	Differential expression profile of gluten-specific T cells identified by single-cell RNA-seq.
title_short	Differential expression profile of gluten-specific T cells identified by single-cell RNA-seq.
title_full	Differential expression profile of gluten-specific T cells identified by single-cell RNA-seq.
title_fullStr	Differential expression profile of gluten-specific T cells identified by single-cell RNA-seq.
title_full_unstemmed	Differential expression profile of gluten-specific T cells identified by single-cell RNA-seq.
title_sort	differential expression profile of gluten-specific t cells identified by single-cell rna-seq.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/e35c933146b7496389afe831b83ade44
work_keys_str_mv	AT yingyao differentialexpressionprofileofglutenspecifictcellsidentifiedbysinglecellrnaseq AT łukaszwyrozzemski differentialexpressionprofileofglutenspecifictcellsidentifiedbysinglecellrnaseq AT knutealundin differentialexpressionprofileofglutenspecifictcellsidentifiedbysinglecellrnaseq AT geirkjetilsandve differentialexpressionprofileofglutenspecifictcellsidentifiedbysinglecellrnaseq AT shuowangqiao differentialexpressionprofileofglutenspecifictcellsidentifiedbysinglecellrnaseq
_version_	1718374435009855488

Differential expression profile of gluten-specific T cells identified by single-cell RNA-seq.

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