Branched polyethylenimine-grafted-carboxymethyl chitosan copolymer enhances the delivery of pDNA or siRNA in vitro and in vivo

Seong-Cheol Park,* Joung-Pyo Nam,* Young-Min Kim,* Jun-Ho Kim, Jae-Woon Nah, Mi-Kyeong Jang Biomedical Polymer Laboratory, Department of Polymer Science and Engineering, Sunchon National University, Suncheon, Republic of Korea *These authors contributed equally to this work Abstract: To generate a g...

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Autores principales:	Park SC, Nam JP, Kim YM, Kim JH, Nah JW, Jang MK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e35d7778b6d44778984fa2e2dbfd0f97
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spelling	oai:doaj.org-article:e35d7778b6d44778984fa2e2dbfd0f972021-12-02T05:02:09ZBranched polyethylenimine-grafted-carboxymethyl chitosan copolymer enhances the delivery of pDNA or siRNA in vitro and in vivo1176-91141178-2013https://doaj.org/article/e35d7778b6d44778984fa2e2dbfd0f972013-09-01T00:00:00Zhttp://www.dovepress.com/branched-polyethylenimine-grafted-carboxymethyl-chitosan-copolymer-enh-a14492https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Seong-Cheol Park,* Joung-Pyo Nam,* Young-Min Kim,* Jun-Ho Kim, Jae-Woon Nah, Mi-Kyeong Jang Biomedical Polymer Laboratory, Department of Polymer Science and Engineering, Sunchon National University, Suncheon, Republic of Korea *These authors contributed equally to this work Abstract: To generate a good carrier for gene transfection, O-carboxymethyl chitosan-graft-branched polyethylenimine (OCMPEI) copolymers were synthesized by increasing the weight percentage of branched polyethylenimine conjugated to the carboxyl groups of O-carboxymethyl chitosan. These spherical polyplexes with plasmid deoxyribonucleic acid (pDNA) or small interfering ribonucleic acid (siRNA) had diameters of ~200–300 nm or ~10–25 nm, respectively, and displayed significant transfection efficiency in normal and tumor cells. In particular, expression of green fluorescent protein (GFP) following pDNA transfection was effectively suppressed by delivery of GFP-specific siRNA with the same copolymer. The optimized copolymer and polyplexes were nontoxic in vitro and in vivo. The use of endocytosis inhibitors to investigate the mechanisms of transfection of the polyplexes suggested the involvement of macropinocytosis. An in vivo study in mice showed excellent GFP expression in the lung, kidney, and liver. The results demonstrated that the OCMPEI copolymer prepared in this study is a promising carrier for in vitro and in vivo gene delivery applications. Keywords: chitosan, branched polyethylenimine, gene transfection, cytotoxicity, endocytosisPark SCNam JPKim YMKim JHNah JWJang MKDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss Issue 1, Pp 3663-3677 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Park SC Nam JP Kim YM Kim JH Nah JW Jang MK Branched polyethylenimine-grafted-carboxymethyl chitosan copolymer enhances the delivery of pDNA or siRNA in vitro and in vivo
description	Seong-Cheol Park,* Joung-Pyo Nam,* Young-Min Kim,* Jun-Ho Kim, Jae-Woon Nah, Mi-Kyeong Jang Biomedical Polymer Laboratory, Department of Polymer Science and Engineering, Sunchon National University, Suncheon, Republic of Korea *These authors contributed equally to this work Abstract: To generate a good carrier for gene transfection, O-carboxymethyl chitosan-graft-branched polyethylenimine (OCMPEI) copolymers were synthesized by increasing the weight percentage of branched polyethylenimine conjugated to the carboxyl groups of O-carboxymethyl chitosan. These spherical polyplexes with plasmid deoxyribonucleic acid (pDNA) or small interfering ribonucleic acid (siRNA) had diameters of ~200–300 nm or ~10–25 nm, respectively, and displayed significant transfection efficiency in normal and tumor cells. In particular, expression of green fluorescent protein (GFP) following pDNA transfection was effectively suppressed by delivery of GFP-specific siRNA with the same copolymer. The optimized copolymer and polyplexes were nontoxic in vitro and in vivo. The use of endocytosis inhibitors to investigate the mechanisms of transfection of the polyplexes suggested the involvement of macropinocytosis. An in vivo study in mice showed excellent GFP expression in the lung, kidney, and liver. The results demonstrated that the OCMPEI copolymer prepared in this study is a promising carrier for in vitro and in vivo gene delivery applications. Keywords: chitosan, branched polyethylenimine, gene transfection, cytotoxicity, endocytosis
format	article
author	Park SC Nam JP Kim YM Kim JH Nah JW Jang MK
author_facet	Park SC Nam JP Kim YM Kim JH Nah JW Jang MK
author_sort	Park SC
title	Branched polyethylenimine-grafted-carboxymethyl chitosan copolymer enhances the delivery of pDNA or siRNA in vitro and in vivo
title_short	Branched polyethylenimine-grafted-carboxymethyl chitosan copolymer enhances the delivery of pDNA or siRNA in vitro and in vivo
title_full	Branched polyethylenimine-grafted-carboxymethyl chitosan copolymer enhances the delivery of pDNA or siRNA in vitro and in vivo
title_fullStr	Branched polyethylenimine-grafted-carboxymethyl chitosan copolymer enhances the delivery of pDNA or siRNA in vitro and in vivo
title_full_unstemmed	Branched polyethylenimine-grafted-carboxymethyl chitosan copolymer enhances the delivery of pDNA or siRNA in vitro and in vivo
title_sort	branched polyethylenimine-grafted-carboxymethyl chitosan copolymer enhances the delivery of pdna or sirna in vitro and in vivo
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/e35d7778b6d44778984fa2e2dbfd0f97
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Branched polyethylenimine-grafted-carboxymethyl chitosan copolymer enhances the delivery of pDNA or siRNA in vitro and in vivo

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