Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection

Abstract We have engineered streptavidin labelled Europium doped fluorescent silica nanoparticles which significantly increased sensitivity without compromising the specificity of the immunoassay. As a proof of concept, a time resolved fluorescence based sandwich immunoassay was developed to detect...

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Autores principales:	L. A. Avinash Chunduri, Aditya Kurdekar, Mohan Kumar Haleyurgirisetty, Eswarappa Pradeep Bulagonda, Venkataramaniah Kamisetti, Indira K. Hewlett
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e37182fe488446fea83b8870b4373791
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spelling	oai:doaj.org-article:e37182fe488446fea83b8870b43737912021-12-02T15:06:17ZFemtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection10.1038/s41598-017-07299-12045-2322https://doaj.org/article/e37182fe488446fea83b8870b43737912017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07299-1https://doaj.org/toc/2045-2322Abstract We have engineered streptavidin labelled Europium doped fluorescent silica nanoparticles which significantly increased sensitivity without compromising the specificity of the immunoassay. As a proof of concept, a time resolved fluorescence based sandwich immunoassay was developed to detect HIV-1 p24 antigen in clinical specimens. The detection range of the silica nanoparticle based immunoassay (SNIA) was found to be between 0.02 to 500 pg/mL in a linear dose dependent manner. SNIA offers 1000 fold enhancement over conventional colorimetric ELISA. Testing of plasma samples that were HIV negative showed no false positive results in the detection of HIV-1 p24 antigen. This highly sensitive p24 assay can help improve blood safety by reducing the antibody negative window period in blood donors in resource limited settings where nucleic acid testing is not practical or feasible. This technology can also be easily transferred to a lab-on-a-chip platform for use in resource limited settings and can also be easily adopted for the detection of other antigens.L. A. Avinash ChunduriAditya KurdekarMohan Kumar HaleyurgirisettyEswarappa Pradeep BulagondaVenkataramaniah KamisettiIndira K. HewlettNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q L. A. Avinash Chunduri Aditya Kurdekar Mohan Kumar Haleyurgirisetty Eswarappa Pradeep Bulagonda Venkataramaniah Kamisetti Indira K. Hewlett Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection
description	Abstract We have engineered streptavidin labelled Europium doped fluorescent silica nanoparticles which significantly increased sensitivity without compromising the specificity of the immunoassay. As a proof of concept, a time resolved fluorescence based sandwich immunoassay was developed to detect HIV-1 p24 antigen in clinical specimens. The detection range of the silica nanoparticle based immunoassay (SNIA) was found to be between 0.02 to 500 pg/mL in a linear dose dependent manner. SNIA offers 1000 fold enhancement over conventional colorimetric ELISA. Testing of plasma samples that were HIV negative showed no false positive results in the detection of HIV-1 p24 antigen. This highly sensitive p24 assay can help improve blood safety by reducing the antibody negative window period in blood donors in resource limited settings where nucleic acid testing is not practical or feasible. This technology can also be easily transferred to a lab-on-a-chip platform for use in resource limited settings and can also be easily adopted for the detection of other antigens.
format	article
author	L. A. Avinash Chunduri Aditya Kurdekar Mohan Kumar Haleyurgirisetty Eswarappa Pradeep Bulagonda Venkataramaniah Kamisetti Indira K. Hewlett
author_facet	L. A. Avinash Chunduri Aditya Kurdekar Mohan Kumar Haleyurgirisetty Eswarappa Pradeep Bulagonda Venkataramaniah Kamisetti Indira K. Hewlett
author_sort	L. A. Avinash Chunduri
title	Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection
title_short	Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection
title_full	Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection
title_fullStr	Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection
title_full_unstemmed	Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection
title_sort	femtogram level sensitivity achieved by surface engineered silica nanoparticles in the early detection of hiv infection
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e37182fe488446fea83b8870b4373791
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Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection

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