Nerve influence on the metabolism of type I and type II diabetic corneal stroma: an in vitro study

Abstract Corneal innervation plays a major role in the pathobiology of diabetic corneal disease. However, innervation impact has mainly been investigated in the context of diabetic epitheliopathy and wound healing. Further studies are warranted in the corneal stroma-nerve interactions. This study un...

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Autores principales:	Amy E. Whelchel, Sarah E. Nicholas, Jian-Xing Ma, Dimitrios Karamichos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e371bc0601aa45068d729c0d4278fd22
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spelling	oai:doaj.org-article:e371bc0601aa45068d729c0d4278fd222021-12-02T16:10:37ZNerve influence on the metabolism of type I and type II diabetic corneal stroma: an in vitro study10.1038/s41598-021-93164-12045-2322https://doaj.org/article/e371bc0601aa45068d729c0d4278fd222021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93164-1https://doaj.org/toc/2045-2322Abstract Corneal innervation plays a major role in the pathobiology of diabetic corneal disease. However, innervation impact has mainly been investigated in the context of diabetic epitheliopathy and wound healing. Further studies are warranted in the corneal stroma-nerve interactions. This study unravels the nerve influence on corneal stroma metabolism. Corneal stromal cells were isolated from healthy (HCFs) and diabetes mellitus (Type1DM and Type2 DM) donors. Cells were cultured on polycarbonate membranes, stimulated by stable Vitamin C, and stroma-only and stroma-nerve co-cultures were investigated for metabolic alterations. Innervated compared to stroma-only constructs exhibited significant alterations in pyrimidine, glycerol phosphate shuttle, electron transport chain and glycolysis. The most highly altered metabolites between healthy and T1DMs innervated were phosphatidylethanolamine biosynthesis, and pyrimidine, methionine, aspartate metabolism. Healthy and T2DMs main pathways included aspartate, glycerol phosphate shuttle, electron transport chain, and gluconeogenesis. The metabolic impact on T1DMs and T2DMs was pyrimidine, purine, aspartate, and methionine. Interestingly, the glucose-6-phosphate and oxaloacetate was higher in T2DMs compared to T1DMs. Our in vitro co-culture model allows the examination of key metabolic pathways corresponding to corneal innervation in the diabetic stroma. These novel findings can pave the way for future studies to fully understand the metabolic distinctions in the diabetic cornea.Amy E. WhelchelSarah E. NicholasJian-Xing MaDimitrios KaramichosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amy E. Whelchel Sarah E. Nicholas Jian-Xing Ma Dimitrios Karamichos Nerve influence on the metabolism of type I and type II diabetic corneal stroma: an in vitro study
description	Abstract Corneal innervation plays a major role in the pathobiology of diabetic corneal disease. However, innervation impact has mainly been investigated in the context of diabetic epitheliopathy and wound healing. Further studies are warranted in the corneal stroma-nerve interactions. This study unravels the nerve influence on corneal stroma metabolism. Corneal stromal cells were isolated from healthy (HCFs) and diabetes mellitus (Type1DM and Type2 DM) donors. Cells were cultured on polycarbonate membranes, stimulated by stable Vitamin C, and stroma-only and stroma-nerve co-cultures were investigated for metabolic alterations. Innervated compared to stroma-only constructs exhibited significant alterations in pyrimidine, glycerol phosphate shuttle, electron transport chain and glycolysis. The most highly altered metabolites between healthy and T1DMs innervated were phosphatidylethanolamine biosynthesis, and pyrimidine, methionine, aspartate metabolism. Healthy and T2DMs main pathways included aspartate, glycerol phosphate shuttle, electron transport chain, and gluconeogenesis. The metabolic impact on T1DMs and T2DMs was pyrimidine, purine, aspartate, and methionine. Interestingly, the glucose-6-phosphate and oxaloacetate was higher in T2DMs compared to T1DMs. Our in vitro co-culture model allows the examination of key metabolic pathways corresponding to corneal innervation in the diabetic stroma. These novel findings can pave the way for future studies to fully understand the metabolic distinctions in the diabetic cornea.
format	article
author	Amy E. Whelchel Sarah E. Nicholas Jian-Xing Ma Dimitrios Karamichos
author_facet	Amy E. Whelchel Sarah E. Nicholas Jian-Xing Ma Dimitrios Karamichos
author_sort	Amy E. Whelchel
title	Nerve influence on the metabolism of type I and type II diabetic corneal stroma: an in vitro study
title_short	Nerve influence on the metabolism of type I and type II diabetic corneal stroma: an in vitro study
title_full	Nerve influence on the metabolism of type I and type II diabetic corneal stroma: an in vitro study
title_fullStr	Nerve influence on the metabolism of type I and type II diabetic corneal stroma: an in vitro study
title_full_unstemmed	Nerve influence on the metabolism of type I and type II diabetic corneal stroma: an in vitro study
title_sort	nerve influence on the metabolism of type i and type ii diabetic corneal stroma: an in vitro study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e371bc0601aa45068d729c0d4278fd22
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Nerve influence on the metabolism of type I and type II diabetic corneal stroma: an in vitro study

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