Concurrent X chromosome inactivation and upregulation during non-human primate preimplantation development revealed by single-cell RNA-sequencing

Abstract In mammals, dosage compensation of X-linked gene expression between males and females is achieved by inactivation of a single X chromosome in females, while upregulation of the single active X in males and females leads to X:autosome dosage balance. Studies in human embryos revealed that ra...

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Autores principales:	Ana Luíza Cidral, Joana C. Moreira de Mello, Joost Gribnau, Lygia V. Pereira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e378d0bcddb740f5be36ef0f11338c65
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spelling	oai:doaj.org-article:e378d0bcddb740f5be36ef0f11338c652021-12-02T16:51:31ZConcurrent X chromosome inactivation and upregulation during non-human primate preimplantation development revealed by single-cell RNA-sequencing10.1038/s41598-021-89175-72045-2322https://doaj.org/article/e378d0bcddb740f5be36ef0f11338c652021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89175-7https://doaj.org/toc/2045-2322Abstract In mammals, dosage compensation of X-linked gene expression between males and females is achieved by inactivation of a single X chromosome in females, while upregulation of the single active X in males and females leads to X:autosome dosage balance. Studies in human embryos revealed that random X chromosome inactivation starts at the preimplantation stage and is not complete by day 12 of development. Alternatively, others proposed that dosage compensation in human preimplantation embryos is achieved by dampening expression from the two X chromosomes in females. Here, we characterize X-linked dosage compensation in another primate, the marmoset (Callithrix jacchus). Analyzing scRNA-seq data from preimplantation embryos, we detected upregulation of XIST at the morula stage, where female embryos presented a significantly higher expression of XIST than males. Moreover, we show an increase of X-linked monoallelically expressed genes in female embryos between the morula and late blastocyst stages, indicative of XCI. Nevertheless, dosage compensation was not achieved by the late blastocyst stage. Finally, we show that X:autosome dosage compensation is achieved at the 8-cell stage, and demonstrate that X chromosome dampening in females does not take place in the marmoset. Our work contributes to the elucidation of primate X-linked dosage compensation.Ana Luíza CidralJoana C. Moreira de MelloJoost GribnauLygia V. PereiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Luíza Cidral Joana C. Moreira de Mello Joost Gribnau Lygia V. Pereira Concurrent X chromosome inactivation and upregulation during non-human primate preimplantation development revealed by single-cell RNA-sequencing
description	Abstract In mammals, dosage compensation of X-linked gene expression between males and females is achieved by inactivation of a single X chromosome in females, while upregulation of the single active X in males and females leads to X:autosome dosage balance. Studies in human embryos revealed that random X chromosome inactivation starts at the preimplantation stage and is not complete by day 12 of development. Alternatively, others proposed that dosage compensation in human preimplantation embryos is achieved by dampening expression from the two X chromosomes in females. Here, we characterize X-linked dosage compensation in another primate, the marmoset (Callithrix jacchus). Analyzing scRNA-seq data from preimplantation embryos, we detected upregulation of XIST at the morula stage, where female embryos presented a significantly higher expression of XIST than males. Moreover, we show an increase of X-linked monoallelically expressed genes in female embryos between the morula and late blastocyst stages, indicative of XCI. Nevertheless, dosage compensation was not achieved by the late blastocyst stage. Finally, we show that X:autosome dosage compensation is achieved at the 8-cell stage, and demonstrate that X chromosome dampening in females does not take place in the marmoset. Our work contributes to the elucidation of primate X-linked dosage compensation.
format	article
author	Ana Luíza Cidral Joana C. Moreira de Mello Joost Gribnau Lygia V. Pereira
author_facet	Ana Luíza Cidral Joana C. Moreira de Mello Joost Gribnau Lygia V. Pereira
author_sort	Ana Luíza Cidral
title	Concurrent X chromosome inactivation and upregulation during non-human primate preimplantation development revealed by single-cell RNA-sequencing
title_short	Concurrent X chromosome inactivation and upregulation during non-human primate preimplantation development revealed by single-cell RNA-sequencing
title_full	Concurrent X chromosome inactivation and upregulation during non-human primate preimplantation development revealed by single-cell RNA-sequencing
title_fullStr	Concurrent X chromosome inactivation and upregulation during non-human primate preimplantation development revealed by single-cell RNA-sequencing
title_full_unstemmed	Concurrent X chromosome inactivation and upregulation during non-human primate preimplantation development revealed by single-cell RNA-sequencing
title_sort	concurrent x chromosome inactivation and upregulation during non-human primate preimplantation development revealed by single-cell rna-sequencing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e378d0bcddb740f5be36ef0f11338c65
work_keys_str_mv	AT analuizacidral concurrentxchromosomeinactivationandupregulationduringnonhumanprimatepreimplantationdevelopmentrevealedbysinglecellrnasequencing AT joanacmoreirademello concurrentxchromosomeinactivationandupregulationduringnonhumanprimatepreimplantationdevelopmentrevealedbysinglecellrnasequencing AT joostgribnau concurrentxchromosomeinactivationandupregulationduringnonhumanprimatepreimplantationdevelopmentrevealedbysinglecellrnasequencing AT lygiavpereira concurrentxchromosomeinactivationandupregulationduringnonhumanprimatepreimplantationdevelopmentrevealedbysinglecellrnasequencing
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Concurrent X chromosome inactivation and upregulation during non-human primate preimplantation development revealed by single-cell RNA-sequencing

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