Probing the active fraction of soil microbiomes using BONCAT-FACS

Probing the active fraction of soil microbiomes using BONCAT-FACS

Standard DNA-based analyses of microbial communities cannot distinguish between active microbes and dead or dormant cells. Here, Couradeau et al. use BONCAT (bioorthogonal non-canonical amino acid tagging), flow cytometry, and 16S rRNA gene amplicon sequencing to identify active microbial cells in s...

Full description

Saved in:
Bibliographic Details
Main Authors: Estelle Couradeau, Joelle Sasse, Danielle Goudeau, Nandita Nath, Terry C. Hazen, Ben P. Bowen, Romy Chakraborty, Rex R. Malmstrom, Trent R. Northen
Format: article
Language:EN
Published: Nature Portfolio 2019
Subjects:
Science
Q
Online Access:https://doaj.org/article/e378eb1778e44890a5e8c1576e06428f
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Standard DNA-based analyses of microbial communities cannot distinguish between active microbes and dead or dormant cells. Here, Couradeau et al. use BONCAT (bioorthogonal non-canonical amino acid tagging), flow cytometry, and 16S rRNA gene amplicon sequencing to identify active microbial cells in soils.

Similar Items