Probing the active fraction of soil microbiomes using BONCAT-FACS

Probing the active fraction of soil microbiomes using BONCAT-FACS

Standard DNA-based analyses of microbial communities cannot distinguish between active microbes and dead or dormant cells. Here, Couradeau et al. use BONCAT (bioorthogonal non-canonical amino acid tagging), flow cytometry, and 16S rRNA gene amplicon sequencing to identify active microbial cells in s...

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Auteurs principaux: Estelle Couradeau, Joelle Sasse, Danielle Goudeau, Nandita Nath, Terry C. Hazen, Ben P. Bowen, Romy Chakraborty, Rex R. Malmstrom, Trent R. Northen
Format: article
Langue:EN
Publié: Nature Portfolio 2019
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Accès en ligne:https://doaj.org/article/e378eb1778e44890a5e8c1576e06428f
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spelling oai:doaj.org-article:e378eb1778e44890a5e8c1576e06428f2021-12-02T16:57:32ZProbing the active fraction of soil microbiomes using BONCAT-FACS10.1038/s41467-019-10542-02041-1723https://doaj.org/article/e378eb1778e44890a5e8c1576e06428f2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10542-0https://doaj.org/toc/2041-1723Standard DNA-based analyses of microbial communities cannot distinguish between active microbes and dead or dormant cells. Here, Couradeau et al. use BONCAT (bioorthogonal non-canonical amino acid tagging), flow cytometry, and 16S rRNA gene amplicon sequencing to identify active microbial cells in soils.Estelle CouradeauJoelle SasseDanielle GoudeauNandita NathTerry C. HazenBen P. BowenRomy ChakrabortyRex R. MalmstromTrent R. NorthenNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Estelle Couradeau
Joelle Sasse
Danielle Goudeau
Nandita Nath
Terry C. Hazen
Ben P. Bowen
Romy Chakraborty
Rex R. Malmstrom
Trent R. Northen
Probing the active fraction of soil microbiomes using BONCAT-FACS
description Standard DNA-based analyses of microbial communities cannot distinguish between active microbes and dead or dormant cells. Here, Couradeau et al. use BONCAT (bioorthogonal non-canonical amino acid tagging), flow cytometry, and 16S rRNA gene amplicon sequencing to identify active microbial cells in soils.
format article
author Estelle Couradeau
Joelle Sasse
Danielle Goudeau
Nandita Nath
Terry C. Hazen
Ben P. Bowen
Romy Chakraborty
Rex R. Malmstrom
Trent R. Northen
author_facet Estelle Couradeau
Joelle Sasse
Danielle Goudeau
Nandita Nath
Terry C. Hazen
Ben P. Bowen
Romy Chakraborty
Rex R. Malmstrom
Trent R. Northen
author_sort Estelle Couradeau
title Probing the active fraction of soil microbiomes using BONCAT-FACS
title_short Probing the active fraction of soil microbiomes using BONCAT-FACS
title_full Probing the active fraction of soil microbiomes using BONCAT-FACS
title_fullStr Probing the active fraction of soil microbiomes using BONCAT-FACS
title_full_unstemmed Probing the active fraction of soil microbiomes using BONCAT-FACS
title_sort probing the active fraction of soil microbiomes using boncat-facs
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/e378eb1778e44890a5e8c1576e06428f
work_keys_str_mv AT estellecouradeau probingtheactivefractionofsoilmicrobiomesusingboncatfacs
AT joellesasse probingtheactivefractionofsoilmicrobiomesusingboncatfacs
AT daniellegoudeau probingtheactivefractionofsoilmicrobiomesusingboncatfacs
AT nanditanath probingtheactivefractionofsoilmicrobiomesusingboncatfacs
AT terrychazen probingtheactivefractionofsoilmicrobiomesusingboncatfacs
AT benpbowen probingtheactivefractionofsoilmicrobiomesusingboncatfacs
AT romychakraborty probingtheactivefractionofsoilmicrobiomesusingboncatfacs
AT rexrmalmstrom probingtheactivefractionofsoilmicrobiomesusingboncatfacs
AT trentrnorthen probingtheactivefractionofsoilmicrobiomesusingboncatfacs
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