Probing the active fraction of soil microbiomes using BONCAT-FACS
Standard DNA-based analyses of microbial communities cannot distinguish between active microbes and dead or dormant cells. Here, Couradeau et al. use BONCAT (bioorthogonal non-canonical amino acid tagging), flow cytometry, and 16S rRNA gene amplicon sequencing to identify active microbial cells in s...
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Nature Portfolio
2019
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oai:doaj.org-article:e378eb1778e44890a5e8c1576e06428f2021-12-02T16:57:32ZProbing the active fraction of soil microbiomes using BONCAT-FACS10.1038/s41467-019-10542-02041-1723https://doaj.org/article/e378eb1778e44890a5e8c1576e06428f2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10542-0https://doaj.org/toc/2041-1723Standard DNA-based analyses of microbial communities cannot distinguish between active microbes and dead or dormant cells. Here, Couradeau et al. use BONCAT (bioorthogonal non-canonical amino acid tagging), flow cytometry, and 16S rRNA gene amplicon sequencing to identify active microbial cells in soils.Estelle CouradeauJoelle SasseDanielle GoudeauNandita NathTerry C. HazenBen P. BowenRomy ChakrabortyRex R. MalmstromTrent R. NorthenNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019) |
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Science Q Estelle Couradeau Joelle Sasse Danielle Goudeau Nandita Nath Terry C. Hazen Ben P. Bowen Romy Chakraborty Rex R. Malmstrom Trent R. Northen Probing the active fraction of soil microbiomes using BONCAT-FACS |
description |
Standard DNA-based analyses of microbial communities cannot distinguish between active microbes and dead or dormant cells. Here, Couradeau et al. use BONCAT (bioorthogonal non-canonical amino acid tagging), flow cytometry, and 16S rRNA gene amplicon sequencing to identify active microbial cells in soils. |
format |
article |
author |
Estelle Couradeau Joelle Sasse Danielle Goudeau Nandita Nath Terry C. Hazen Ben P. Bowen Romy Chakraborty Rex R. Malmstrom Trent R. Northen |
author_facet |
Estelle Couradeau Joelle Sasse Danielle Goudeau Nandita Nath Terry C. Hazen Ben P. Bowen Romy Chakraborty Rex R. Malmstrom Trent R. Northen |
author_sort |
Estelle Couradeau |
title |
Probing the active fraction of soil microbiomes using BONCAT-FACS |
title_short |
Probing the active fraction of soil microbiomes using BONCAT-FACS |
title_full |
Probing the active fraction of soil microbiomes using BONCAT-FACS |
title_fullStr |
Probing the active fraction of soil microbiomes using BONCAT-FACS |
title_full_unstemmed |
Probing the active fraction of soil microbiomes using BONCAT-FACS |
title_sort |
probing the active fraction of soil microbiomes using boncat-facs |
publisher |
Nature Portfolio |
publishDate |
2019 |
url |
https://doaj.org/article/e378eb1778e44890a5e8c1576e06428f |
work_keys_str_mv |
AT estellecouradeau probingtheactivefractionofsoilmicrobiomesusingboncatfacs AT joellesasse probingtheactivefractionofsoilmicrobiomesusingboncatfacs AT daniellegoudeau probingtheactivefractionofsoilmicrobiomesusingboncatfacs AT nanditanath probingtheactivefractionofsoilmicrobiomesusingboncatfacs AT terrychazen probingtheactivefractionofsoilmicrobiomesusingboncatfacs AT benpbowen probingtheactivefractionofsoilmicrobiomesusingboncatfacs AT romychakraborty probingtheactivefractionofsoilmicrobiomesusingboncatfacs AT rexrmalmstrom probingtheactivefractionofsoilmicrobiomesusingboncatfacs AT trentrnorthen probingtheactivefractionofsoilmicrobiomesusingboncatfacs |
_version_ |
1718382536077344768 |