Probing the active fraction of soil microbiomes using BONCAT-FACS
Standard DNA-based analyses of microbial communities cannot distinguish between active microbes and dead or dormant cells. Here, Couradeau et al. use BONCAT (bioorthogonal non-canonical amino acid tagging), flow cytometry, and 16S rRNA gene amplicon sequencing to identify active microbial cells in s...
Guardado en:
Autores principales: | Estelle Couradeau, Joelle Sasse, Danielle Goudeau, Nandita Nath, Terry C. Hazen, Ben P. Bowen, Romy Chakraborty, Rex R. Malmstrom, Trent R. Northen |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2019
|
Materias: | |
Acceso en línea: | https://doaj.org/article/e378eb1778e44890a5e8c1576e06428f |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
Bacteria increase arid-land soil surface temperature through the production of sunscreens
por: Estelle Couradeau, et al.
Publicado: (2016) -
Large Blooms of <italic toggle="yes">Bacillales</italic> (<italic toggle="yes">Firmicutes</italic>) Underlie the Response to Wetting of Cyanobacterial Biocrusts at Various Stages of Maturity
por: Ulas Karaoz, et al.
Publicado: (2018) -
Hidden diversity of soil giant viruses
por: Frederik Schulz, et al.
Publicado: (2018) -
A rapid FACS-based strategy to isolate human gene knockin and knockout clones.
por: João F Mata, et al.
Publicado: (2012) -
Publisher Correction: A FACS-based approach to obtain viable eosinophils from human adipose tissue
por: James D. Hernandez, et al.
Publicado: (2021)