Electrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays

Abstract Dopamine is a key molecule in neurotransmission and has been known to be responsible for several neurological diseases. Hence, its sensitive and selective detection is important for the early diagnosis of diseases related to abnormal levels of dopamine. In this study, we reported a new cyli...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Da-Seul Kim, Ee-Seul Kang, Seungho Baek, Sung-Sik Choo, Yong-Ho Chung, Donghyun Lee, Junhong Min, Tae-Hyung Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Dopamine Detection Human Neuronal Cells Nanocylinders Potassium Citrate Monohydrate Real Human Plasma Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e37d854f0a9945508d7ff25d159a956f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e37d854f0a9945508d7ff25d159a956f
record_format	dspace
spelling	oai:doaj.org-article:e37d854f0a9945508d7ff25d159a956f2021-12-02T15:07:56ZElectrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays10.1038/s41598-018-32477-02045-2322https://doaj.org/article/e37d854f0a9945508d7ff25d159a956f2018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32477-0https://doaj.org/toc/2045-2322Abstract Dopamine is a key molecule in neurotransmission and has been known to be responsible for several neurological diseases. Hence, its sensitive and selective detection is important for the early diagnosis of diseases related to abnormal levels of dopamine. In this study, we reported a new cylindrical gold nanoelectrode (CAuNE) platform fabricated via sequential laser interference lithography and electrochemical deposition. Among the fabricated electrodes, CAuNEs with a diameter of 700 nm, 150 s deposited, was found to be the best for electrochemical dopamine detection. According to cyclic voltammetry results, the linear range of the CAuNE-700 nm was 1–100 µM of dopamine with a limit of detection (LOD) of 5.83 µM. Moreover, owing to the homogeneous periodic features of CAuNEs, human neural cells were successfully cultured and maintained for more than 5 days in vitro without the use of any extracellular matrix proteins and dopamine was detectable in the presence of these cells on the electrode. Therefore, we concluded that the developed dopamine sensing platform CAuNE can be used for many applications including early diagnosis of neurological diseases; function tests of dopaminergic neurons derived from various stem cell sources; and toxicity assessments of drugs, chemicals, and nanomaterials on human neuronal cells.Da-Seul KimEe-Seul KangSeungho BaekSung-Sik ChooYong-Ho ChungDonghyun LeeJunhong MinTae-Hyung KimNature PortfolioarticleDopamine DetectionHuman Neuronal CellsNanocylindersPotassium Citrate MonohydrateReal Human PlasmaMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dopamine Detection Human Neuronal Cells Nanocylinders Potassium Citrate Monohydrate Real Human Plasma Medicine R Science Q
spellingShingle	Dopamine Detection Human Neuronal Cells Nanocylinders Potassium Citrate Monohydrate Real Human Plasma Medicine R Science Q Da-Seul Kim Ee-Seul Kang Seungho Baek Sung-Sik Choo Yong-Ho Chung Donghyun Lee Junhong Min Tae-Hyung Kim Electrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays
description	Abstract Dopamine is a key molecule in neurotransmission and has been known to be responsible for several neurological diseases. Hence, its sensitive and selective detection is important for the early diagnosis of diseases related to abnormal levels of dopamine. In this study, we reported a new cylindrical gold nanoelectrode (CAuNE) platform fabricated via sequential laser interference lithography and electrochemical deposition. Among the fabricated electrodes, CAuNEs with a diameter of 700 nm, 150 s deposited, was found to be the best for electrochemical dopamine detection. According to cyclic voltammetry results, the linear range of the CAuNE-700 nm was 1–100 µM of dopamine with a limit of detection (LOD) of 5.83 µM. Moreover, owing to the homogeneous periodic features of CAuNEs, human neural cells were successfully cultured and maintained for more than 5 days in vitro without the use of any extracellular matrix proteins and dopamine was detectable in the presence of these cells on the electrode. Therefore, we concluded that the developed dopamine sensing platform CAuNE can be used for many applications including early diagnosis of neurological diseases; function tests of dopaminergic neurons derived from various stem cell sources; and toxicity assessments of drugs, chemicals, and nanomaterials on human neuronal cells.
format	article
author	Da-Seul Kim Ee-Seul Kang Seungho Baek Sung-Sik Choo Yong-Ho Chung Donghyun Lee Junhong Min Tae-Hyung Kim
author_facet	Da-Seul Kim Ee-Seul Kang Seungho Baek Sung-Sik Choo Yong-Ho Chung Donghyun Lee Junhong Min Tae-Hyung Kim
author_sort	Da-Seul Kim
title	Electrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays
title_short	Electrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays
title_full	Electrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays
title_fullStr	Electrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays
title_full_unstemmed	Electrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays
title_sort	electrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/e37d854f0a9945508d7ff25d159a956f
work_keys_str_mv	AT daseulkim electrochemicaldetectionofdopamineusingperiodiccylindricalgoldnanoelectrodearrays AT eeseulkang electrochemicaldetectionofdopamineusingperiodiccylindricalgoldnanoelectrodearrays AT seunghobaek electrochemicaldetectionofdopamineusingperiodiccylindricalgoldnanoelectrodearrays AT sungsikchoo electrochemicaldetectionofdopamineusingperiodiccylindricalgoldnanoelectrodearrays AT yonghochung electrochemicaldetectionofdopamineusingperiodiccylindricalgoldnanoelectrodearrays AT donghyunlee electrochemicaldetectionofdopamineusingperiodiccylindricalgoldnanoelectrodearrays AT junhongmin electrochemicaldetectionofdopamineusingperiodiccylindricalgoldnanoelectrodearrays AT taehyungkim electrochemicaldetectionofdopamineusingperiodiccylindricalgoldnanoelectrodearrays
_version_	1718388353056899072

Electrochemical detection of dopamine using periodic cylindrical gold nanoelectrode arrays

Ejemplares similares