Identification and Analysis of Zinc Efficiency-Associated Loci in Maize

Zinc (Zn) deficiency, a globally predominant micronutrient disorder in crops and humans, reduces crop yields and adversely impacts human health. Despite numerous studies on the physiological mechanisms underlying Zn deficiency tolerance, its genetic basis of molecular mechanism is still poorly under...

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Autores principales:	Jianqin Xu, Xuejie Wang, Huaqing Zhu, Futong Yu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	maize (Zea mays L.) quantitative trait locus (QTL) zinc (Zn) deficiency tolerance ZRT/IRT-like protein (ZIP) candidate genes Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/e37dd9566ab54be9b1c2196e0f2eedaf
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spelling	oai:doaj.org-article:e37dd9566ab54be9b1c2196e0f2eedaf2021-11-15T07:00:22ZIdentification and Analysis of Zinc Efficiency-Associated Loci in Maize1664-462X10.3389/fpls.2021.739282https://doaj.org/article/e37dd9566ab54be9b1c2196e0f2eedaf2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.739282/fullhttps://doaj.org/toc/1664-462XZinc (Zn) deficiency, a globally predominant micronutrient disorder in crops and humans, reduces crop yields and adversely impacts human health. Despite numerous studies on the physiological mechanisms underlying Zn deficiency tolerance, its genetic basis of molecular mechanism is still poorly understood. Thus, the Zn efficiency of 20 maize inbred lines was evaluated, and a quantitative trait locus (QTL) analysis was performed in the recombination inbred line population derived from the most Zn-efficient (Ye478) and Zn-inefficient inbred line (Wu312) to identify the candidate genes associated with Zn deficiency tolerance. On this basis, we analyzed the expression of ZmZIP1-ZmZIP8. Thirteen QTLs for the traits associated with Zn deficiency tolerance were detected, explaining 7.6–63.5% of the phenotypic variation. The genes responsible for Zn uptake and transport across membranes (ZmZIP3, ZmHMA3, ZmHMA4) were identified, which probably form a sophisticated network to regulate the uptake, translocation, and redistribution of Zn. Additionally, we identified the genes involved in the indole-3-acetic acid (IAA) biosynthesis (ZmIGPS) and auxin-dependent gene regulation (ZmIAA). Notably, a high upregulation of ZmZIP3 was found in the Zn-deficient root of Ye478, but not in that of Wu312. Additionally, ZmZIP4, ZmZIP5, and ZmZIP7 were up-regulated in the Zn-deficient roots of Ye478 and Wu312. Our findings provide a new insight into the genetic basis of Zn deficiency tolerance.Jianqin XuXuejie WangHuaqing ZhuFutong YuFrontiers Media S.A.articlemaize (Zea mays L.)quantitative trait locus (QTL)zinc (Zn) deficiency toleranceZRT/IRT-like protein (ZIP)candidate genesPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	maize (Zea mays L.) quantitative trait locus (QTL) zinc (Zn) deficiency tolerance ZRT/IRT-like protein (ZIP) candidate genes Plant culture SB1-1110
spellingShingle	maize (Zea mays L.) quantitative trait locus (QTL) zinc (Zn) deficiency tolerance ZRT/IRT-like protein (ZIP) candidate genes Plant culture SB1-1110 Jianqin Xu Xuejie Wang Huaqing Zhu Futong Yu Identification and Analysis of Zinc Efficiency-Associated Loci in Maize
description	Zinc (Zn) deficiency, a globally predominant micronutrient disorder in crops and humans, reduces crop yields and adversely impacts human health. Despite numerous studies on the physiological mechanisms underlying Zn deficiency tolerance, its genetic basis of molecular mechanism is still poorly understood. Thus, the Zn efficiency of 20 maize inbred lines was evaluated, and a quantitative trait locus (QTL) analysis was performed in the recombination inbred line population derived from the most Zn-efficient (Ye478) and Zn-inefficient inbred line (Wu312) to identify the candidate genes associated with Zn deficiency tolerance. On this basis, we analyzed the expression of ZmZIP1-ZmZIP8. Thirteen QTLs for the traits associated with Zn deficiency tolerance were detected, explaining 7.6–63.5% of the phenotypic variation. The genes responsible for Zn uptake and transport across membranes (ZmZIP3, ZmHMA3, ZmHMA4) were identified, which probably form a sophisticated network to regulate the uptake, translocation, and redistribution of Zn. Additionally, we identified the genes involved in the indole-3-acetic acid (IAA) biosynthesis (ZmIGPS) and auxin-dependent gene regulation (ZmIAA). Notably, a high upregulation of ZmZIP3 was found in the Zn-deficient root of Ye478, but not in that of Wu312. Additionally, ZmZIP4, ZmZIP5, and ZmZIP7 were up-regulated in the Zn-deficient roots of Ye478 and Wu312. Our findings provide a new insight into the genetic basis of Zn deficiency tolerance.
format	article
author	Jianqin Xu Xuejie Wang Huaqing Zhu Futong Yu
author_facet	Jianqin Xu Xuejie Wang Huaqing Zhu Futong Yu
author_sort	Jianqin Xu
title	Identification and Analysis of Zinc Efficiency-Associated Loci in Maize
title_short	Identification and Analysis of Zinc Efficiency-Associated Loci in Maize
title_full	Identification and Analysis of Zinc Efficiency-Associated Loci in Maize
title_fullStr	Identification and Analysis of Zinc Efficiency-Associated Loci in Maize
title_full_unstemmed	Identification and Analysis of Zinc Efficiency-Associated Loci in Maize
title_sort	identification and analysis of zinc efficiency-associated loci in maize
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e37dd9566ab54be9b1c2196e0f2eedaf
work_keys_str_mv	AT jianqinxu identificationandanalysisofzincefficiencyassociatedlociinmaize AT xuejiewang identificationandanalysisofzincefficiencyassociatedlociinmaize AT huaqingzhu identificationandanalysisofzincefficiencyassociatedlociinmaize AT futongyu identificationandanalysisofzincefficiencyassociatedlociinmaize
_version_	1718428527129264128

Identification and Analysis of Zinc Efficiency-Associated Loci in Maize

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