CaMK II Inhibition Attenuates ROS Dependent Necroptosis in Acinar Cells and Protects against Acute Pancreatitis in Mice

As a calcium-regulated protein, CaMK II is closely related to cell death, and it participates in the development of pathological processes such as reperfusion injury, myocardial infarction, and oligodendrocyte death. The function of CaMK II activation in acute pancreatitis (AP) remains unclear. In o...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Qingtian Zhu, Lu Hao, Qinhao Shen, Jiajia Pan, Weili Liu, Weijuan Gong, Lianghao Hu, Weiming Xiao, Mei Wang, Xinnong Liu, Yanbing Ding, Guotao Lu
Formato: article
Lenguaje:EN
Publicado: Hindawi Limited 2021
Materias:
Acceso en línea:https://doaj.org/article/e37f532b4270439d930e8af1b7acd0b0
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:e37f532b4270439d930e8af1b7acd0b0
record_format dspace
spelling oai:doaj.org-article:e37f532b4270439d930e8af1b7acd0b02021-11-29T00:55:54ZCaMK II Inhibition Attenuates ROS Dependent Necroptosis in Acinar Cells and Protects against Acute Pancreatitis in Mice1942-099410.1155/2021/4187398https://doaj.org/article/e37f532b4270439d930e8af1b7acd0b02021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/4187398https://doaj.org/toc/1942-0994As a calcium-regulated protein, CaMK II is closely related to cell death, and it participates in the development of pathological processes such as reperfusion injury, myocardial infarction, and oligodendrocyte death. The function of CaMK II activation in acute pancreatitis (AP) remains unclear. In our study, we confirmed that the expression of p-CaMK II was increased significantly and consistently in injured pancreatic tissues after caerulein-induced AP. Then, we found that KN93, an inhibitor of CaMK II, could mitigate the histopathological manifestations in pancreatic tissues, reduce serum levels of enzymology, and decrease oxidative stress products. Accordingly, we elucidated the effect of KN93 in vitro and found that KN93 had a protective effect on the pancreatic acinar cell necroptosis pathway by inhibiting the production of ROS and decreasing the expression of RIP3 and p-MLKL. In addition, we identified the protective effect of KN93 on AP through another mouse model induced by pancreatic duct ligation (PDL). Together, these data demonstrated that CaMK II participates in the development of AP and that inhibiting CaMK II activation could protect against AP by reducing acinar cell necroptosis, which may provide a new idea target for the prevention and treatment of AP in the clinic.Qingtian ZhuLu HaoQinhao ShenJiajia PanWeili LiuWeijuan GongLianghao HuWeiming XiaoMei WangXinnong LiuYanbing DingGuotao LuHindawi LimitedarticleCytologyQH573-671ENOxidative Medicine and Cellular Longevity, Vol 2021 (2021)
institution DOAJ
collection DOAJ
language EN
topic Cytology
QH573-671
spellingShingle Cytology
QH573-671
Qingtian Zhu
Lu Hao
Qinhao Shen
Jiajia Pan
Weili Liu
Weijuan Gong
Lianghao Hu
Weiming Xiao
Mei Wang
Xinnong Liu
Yanbing Ding
Guotao Lu
CaMK II Inhibition Attenuates ROS Dependent Necroptosis in Acinar Cells and Protects against Acute Pancreatitis in Mice
description As a calcium-regulated protein, CaMK II is closely related to cell death, and it participates in the development of pathological processes such as reperfusion injury, myocardial infarction, and oligodendrocyte death. The function of CaMK II activation in acute pancreatitis (AP) remains unclear. In our study, we confirmed that the expression of p-CaMK II was increased significantly and consistently in injured pancreatic tissues after caerulein-induced AP. Then, we found that KN93, an inhibitor of CaMK II, could mitigate the histopathological manifestations in pancreatic tissues, reduce serum levels of enzymology, and decrease oxidative stress products. Accordingly, we elucidated the effect of KN93 in vitro and found that KN93 had a protective effect on the pancreatic acinar cell necroptosis pathway by inhibiting the production of ROS and decreasing the expression of RIP3 and p-MLKL. In addition, we identified the protective effect of KN93 on AP through another mouse model induced by pancreatic duct ligation (PDL). Together, these data demonstrated that CaMK II participates in the development of AP and that inhibiting CaMK II activation could protect against AP by reducing acinar cell necroptosis, which may provide a new idea target for the prevention and treatment of AP in the clinic.
format article
author Qingtian Zhu
Lu Hao
Qinhao Shen
Jiajia Pan
Weili Liu
Weijuan Gong
Lianghao Hu
Weiming Xiao
Mei Wang
Xinnong Liu
Yanbing Ding
Guotao Lu
author_facet Qingtian Zhu
Lu Hao
Qinhao Shen
Jiajia Pan
Weili Liu
Weijuan Gong
Lianghao Hu
Weiming Xiao
Mei Wang
Xinnong Liu
Yanbing Ding
Guotao Lu
author_sort Qingtian Zhu
title CaMK II Inhibition Attenuates ROS Dependent Necroptosis in Acinar Cells and Protects against Acute Pancreatitis in Mice
title_short CaMK II Inhibition Attenuates ROS Dependent Necroptosis in Acinar Cells and Protects against Acute Pancreatitis in Mice
title_full CaMK II Inhibition Attenuates ROS Dependent Necroptosis in Acinar Cells and Protects against Acute Pancreatitis in Mice
title_fullStr CaMK II Inhibition Attenuates ROS Dependent Necroptosis in Acinar Cells and Protects against Acute Pancreatitis in Mice
title_full_unstemmed CaMK II Inhibition Attenuates ROS Dependent Necroptosis in Acinar Cells and Protects against Acute Pancreatitis in Mice
title_sort camk ii inhibition attenuates ros dependent necroptosis in acinar cells and protects against acute pancreatitis in mice
publisher Hindawi Limited
publishDate 2021
url https://doaj.org/article/e37f532b4270439d930e8af1b7acd0b0
work_keys_str_mv AT qingtianzhu camkiiinhibitionattenuatesrosdependentnecroptosisinacinarcellsandprotectsagainstacutepancreatitisinmice
AT luhao camkiiinhibitionattenuatesrosdependentnecroptosisinacinarcellsandprotectsagainstacutepancreatitisinmice
AT qinhaoshen camkiiinhibitionattenuatesrosdependentnecroptosisinacinarcellsandprotectsagainstacutepancreatitisinmice
AT jiajiapan camkiiinhibitionattenuatesrosdependentnecroptosisinacinarcellsandprotectsagainstacutepancreatitisinmice
AT weililiu camkiiinhibitionattenuatesrosdependentnecroptosisinacinarcellsandprotectsagainstacutepancreatitisinmice
AT weijuangong camkiiinhibitionattenuatesrosdependentnecroptosisinacinarcellsandprotectsagainstacutepancreatitisinmice
AT lianghaohu camkiiinhibitionattenuatesrosdependentnecroptosisinacinarcellsandprotectsagainstacutepancreatitisinmice
AT weimingxiao camkiiinhibitionattenuatesrosdependentnecroptosisinacinarcellsandprotectsagainstacutepancreatitisinmice
AT meiwang camkiiinhibitionattenuatesrosdependentnecroptosisinacinarcellsandprotectsagainstacutepancreatitisinmice
AT xinnongliu camkiiinhibitionattenuatesrosdependentnecroptosisinacinarcellsandprotectsagainstacutepancreatitisinmice
AT yanbingding camkiiinhibitionattenuatesrosdependentnecroptosisinacinarcellsandprotectsagainstacutepancreatitisinmice
AT guotaolu camkiiinhibitionattenuatesrosdependentnecroptosisinacinarcellsandprotectsagainstacutepancreatitisinmice
_version_ 1718407804855779328