Identification, expression and target gene analyses of Micrornas in Spodoptera litura.

MicroRNAs (miRNAs) are small RNAs widely present in animals and plants and involved in post-transcriptional regulation of gene transcripts. In this study we identified and validated 58 miRNAs from an EST dataset of Spodoptera litura based on the computational and experimental analysis of sequence co...

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Autores principales:	Zhongchen Rao, Wenyin He, Lin Liu, Sichun Zheng, Lihua Huang, Qili Feng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e38ac956dd08429fa8247aba47cd18a4
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spelling	oai:doaj.org-article:e38ac956dd08429fa8247aba47cd18a42021-11-18T07:17:20ZIdentification, expression and target gene analyses of Micrornas in Spodoptera litura.1932-620310.1371/journal.pone.0037730https://doaj.org/article/e38ac956dd08429fa8247aba47cd18a42012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22662202/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203MicroRNAs (miRNAs) are small RNAs widely present in animals and plants and involved in post-transcriptional regulation of gene transcripts. In this study we identified and validated 58 miRNAs from an EST dataset of Spodoptera litura based on the computational and experimental analysis of sequence conservation and secondary structure of miRNA by comparing the miRNA sequences in the miRbase. RT-PCR was conducted to examine the expression of these miRNAs and stem-loop RT-PCR assay was performed to examine expression of 11 mature miRNAs (out of the 58 putative miRNA) that showed significant changes in different tissues and stages of the insect development. One hundred twenty eight possible target genes against the 11 miRNAs were predicted by using computational methods. Binding of one miRNA (sli-miR-928b) with the three possible target mRNAs was confirmed by Southern blotting, implying its possible function in regulation of the target genes.Zhongchen RaoWenyin HeLin LiuSichun ZhengLihua HuangQili FengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37730 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhongchen Rao Wenyin He Lin Liu Sichun Zheng Lihua Huang Qili Feng Identification, expression and target gene analyses of Micrornas in Spodoptera litura.
description	MicroRNAs (miRNAs) are small RNAs widely present in animals and plants and involved in post-transcriptional regulation of gene transcripts. In this study we identified and validated 58 miRNAs from an EST dataset of Spodoptera litura based on the computational and experimental analysis of sequence conservation and secondary structure of miRNA by comparing the miRNA sequences in the miRbase. RT-PCR was conducted to examine the expression of these miRNAs and stem-loop RT-PCR assay was performed to examine expression of 11 mature miRNAs (out of the 58 putative miRNA) that showed significant changes in different tissues and stages of the insect development. One hundred twenty eight possible target genes against the 11 miRNAs were predicted by using computational methods. Binding of one miRNA (sli-miR-928b) with the three possible target mRNAs was confirmed by Southern blotting, implying its possible function in regulation of the target genes.
format	article
author	Zhongchen Rao Wenyin He Lin Liu Sichun Zheng Lihua Huang Qili Feng
author_facet	Zhongchen Rao Wenyin He Lin Liu Sichun Zheng Lihua Huang Qili Feng
author_sort	Zhongchen Rao
title	Identification, expression and target gene analyses of Micrornas in Spodoptera litura.
title_short	Identification, expression and target gene analyses of Micrornas in Spodoptera litura.
title_full	Identification, expression and target gene analyses of Micrornas in Spodoptera litura.
title_fullStr	Identification, expression and target gene analyses of Micrornas in Spodoptera litura.
title_full_unstemmed	Identification, expression and target gene analyses of Micrornas in Spodoptera litura.
title_sort	identification, expression and target gene analyses of micrornas in spodoptera litura.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e38ac956dd08429fa8247aba47cd18a4
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Identification, expression and target gene analyses of Micrornas in Spodoptera litura.

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