Myelin-associated glycoprotein combined with chitin conduit inhibits painful neuroma formation after sciatic nerve transection

Studies have shown that myelin-associated glycoprotein (MAG) can inhibit axon regeneration after nerve injury. However, the effects of MAG on neuroma formation after peripheral nerve injury remain poorly understood. In this study, local injection of MAG combined with nerve cap made of chitin conduit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wei Pi, Ci Li, Meng Zhang, Wei Zhang, Pei-Xun Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2022
Materias:	autotomy; axon; chitin conduit; fibrosis; myelin-associated glycoprotein; painful neuroma; peripheral nerve; regeneration Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/e3944a0d301847f481d8c0541ad8962b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e3944a0d301847f481d8c0541ad8962b
record_format	dspace
spelling	oai:doaj.org-article:e3944a0d301847f481d8c0541ad8962b2021-11-19T12:16:44ZMyelin-associated glycoprotein combined with chitin conduit inhibits painful neuroma formation after sciatic nerve transection1673-537410.4103/1673-5374.327351https://doaj.org/article/e3944a0d301847f481d8c0541ad8962b2022-01-01T00:00:00Zhttp://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=6;spage=1343;epage=1347;aulast=Pihttps://doaj.org/toc/1673-5374Studies have shown that myelin-associated glycoprotein (MAG) can inhibit axon regeneration after nerve injury. However, the effects of MAG on neuroma formation after peripheral nerve injury remain poorly understood. In this study, local injection of MAG combined with nerve cap made of chitin conduit was used to intervene with the formation of painful neuroma after sciatic nerve transfection in rats. After 8 weeks of combined treatment, the autotomy behaviors were reduced in rats subjected to sciatic nerve transfection, the mRNA expression of nerve growth factor, a pain marker, in the proximal nerve stump was decreased, the density of regenerated axons was decreased, the thickness of the myelin sheath was increased, and the ratio of unmyelinated to myelinated axons was reduced. Moereover, the percentage of collagen fiber area and the percentage of fibrosis marker alpha-smooth muscle actin positive staining area in the proximal nerve stump were decreased. The combined treatment exhibited superior effects in these measures to chitin conduit treatment alone. These findings suggest that MAG combined with chitin conduit synergistically inhibits the formation of painful neuroma after sciatic nerve transection and alleviates neuropathic pain. This study was approved by the Animal Ethics Committee of Peking University People’s Hospital (approval No. 2019PHE027) on December 5, 2019.Wei PiCi LiMeng ZhangWei ZhangPei-Xun ZhangWolters Kluwer Medknow Publicationsarticleautotomy; axon; chitin conduit; fibrosis; myelin-associated glycoprotein; painful neuroma; peripheral nerve; regenerationNeurology. Diseases of the nervous systemRC346-429ENNeural Regeneration Research, Vol 17, Iss 6, Pp 1343-1347 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	autotomy; axon; chitin conduit; fibrosis; myelin-associated glycoprotein; painful neuroma; peripheral nerve; regeneration Neurology. Diseases of the nervous system RC346-429
spellingShingle	autotomy; axon; chitin conduit; fibrosis; myelin-associated glycoprotein; painful neuroma; peripheral nerve; regeneration Neurology. Diseases of the nervous system RC346-429 Wei Pi Ci Li Meng Zhang Wei Zhang Pei-Xun Zhang Myelin-associated glycoprotein combined with chitin conduit inhibits painful neuroma formation after sciatic nerve transection
description	Studies have shown that myelin-associated glycoprotein (MAG) can inhibit axon regeneration after nerve injury. However, the effects of MAG on neuroma formation after peripheral nerve injury remain poorly understood. In this study, local injection of MAG combined with nerve cap made of chitin conduit was used to intervene with the formation of painful neuroma after sciatic nerve transfection in rats. After 8 weeks of combined treatment, the autotomy behaviors were reduced in rats subjected to sciatic nerve transfection, the mRNA expression of nerve growth factor, a pain marker, in the proximal nerve stump was decreased, the density of regenerated axons was decreased, the thickness of the myelin sheath was increased, and the ratio of unmyelinated to myelinated axons was reduced. Moereover, the percentage of collagen fiber area and the percentage of fibrosis marker alpha-smooth muscle actin positive staining area in the proximal nerve stump were decreased. The combined treatment exhibited superior effects in these measures to chitin conduit treatment alone. These findings suggest that MAG combined with chitin conduit synergistically inhibits the formation of painful neuroma after sciatic nerve transection and alleviates neuropathic pain. This study was approved by the Animal Ethics Committee of Peking University People’s Hospital (approval No. 2019PHE027) on December 5, 2019.
format	article
author	Wei Pi Ci Li Meng Zhang Wei Zhang Pei-Xun Zhang
author_facet	Wei Pi Ci Li Meng Zhang Wei Zhang Pei-Xun Zhang
author_sort	Wei Pi
title	Myelin-associated glycoprotein combined with chitin conduit inhibits painful neuroma formation after sciatic nerve transection
title_short	Myelin-associated glycoprotein combined with chitin conduit inhibits painful neuroma formation after sciatic nerve transection
title_full	Myelin-associated glycoprotein combined with chitin conduit inhibits painful neuroma formation after sciatic nerve transection
title_fullStr	Myelin-associated glycoprotein combined with chitin conduit inhibits painful neuroma formation after sciatic nerve transection
title_full_unstemmed	Myelin-associated glycoprotein combined with chitin conduit inhibits painful neuroma formation after sciatic nerve transection
title_sort	myelin-associated glycoprotein combined with chitin conduit inhibits painful neuroma formation after sciatic nerve transection
publisher	Wolters Kluwer Medknow Publications
publishDate	2022
url	https://doaj.org/article/e3944a0d301847f481d8c0541ad8962b
work_keys_str_mv	AT weipi myelinassociatedglycoproteincombinedwithchitinconduitinhibitspainfulneuromaformationaftersciaticnervetransection AT cili myelinassociatedglycoproteincombinedwithchitinconduitinhibitspainfulneuromaformationaftersciaticnervetransection AT mengzhang myelinassociatedglycoproteincombinedwithchitinconduitinhibitspainfulneuromaformationaftersciaticnervetransection AT weizhang myelinassociatedglycoproteincombinedwithchitinconduitinhibitspainfulneuromaformationaftersciaticnervetransection AT peixunzhang myelinassociatedglycoproteincombinedwithchitinconduitinhibitspainfulneuromaformationaftersciaticnervetransection
_version_	1718420102589710336

Myelin-associated glycoprotein combined with chitin conduit inhibits painful neuroma formation after sciatic nerve transection

Ejemplares similares