Initial osteoblast adhesion and subsequent differentiation on zirconia surfaces are regulated by integrins and heparin-sensitive molecule

Feng Luo,1,2 Guang Hong,3,4 Hiroyuki Matsui,2 Kosei Endo,5 Qianbing Wan,1 Keiichi Sasaki2 1State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; 2Division of Advanced Prosthetic Dent...

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Autores principales:	Luo F, Hong G, Matsui H, Endo K, Wan Q, Sasaki K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	zirconia biocompatibility adhesion RGD peptide integrins Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e39485d095a341f4b587b90600da869c
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spelling	oai:doaj.org-article:e39485d095a341f4b587b90600da869c2021-12-02T02:20:01ZInitial osteoblast adhesion and subsequent differentiation on zirconia surfaces are regulated by integrins and heparin-sensitive molecule1178-2013https://doaj.org/article/e39485d095a341f4b587b90600da869c2018-11-01T00:00:00Zhttps://www.dovepress.com/initial-osteoblast-adhesion-and-subsequent-differentiation-on-zirconia-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Feng Luo,1,2 Guang Hong,3,4 Hiroyuki Matsui,2 Kosei Endo,5 Qianbing Wan,1 Keiichi Sasaki2 1State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; 2Division of Advanced Prosthetic Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Japan; 3Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Japan; 4Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia; 5Division of Aging and Geriatric Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Japan Purpose: It is well known that zirconia materials have good biocompatibility; however, little is known regarding the mechanism by which cells attach to these materials. The purpose of this study is to elucidate the mechanism of cell attachment. Materials and methods: In this study, we examined the surface characteristics of ceria-stabilized zirconia/alumina nanocomposite (NANOZR), yttria-stabilized zirconia (Y-TZP) and commercially pure titanium (CpTi), and we evaluated the initial response of osteoblast-like cells to them with different inhibitors. Results: Under the same polishing treatment, the three materials, NANOZR, Y-TZP and CpTi, show similar surface wettability but different surface roughness. Osteoblasts could adhere to the surface of all three materials, and spindle shapes were clearer in serum-containing media compared to PBS and serum-free culture media, suggesting that serum-contained proteins are helpful for the initial cell adhesion and spreading. Cell adhesion and proliferation were disrupted in the presence of EDTA. RGD-peptide interfered with cell proliferation by affecting cell protrusion and stress fibers. Monoclonal antibody against non-RGD type integrin α2β1 enhanced proliferation in Y-TZP, CpTi and culture dish but not in NANOZR. Cell proliferation on NANOZR was specifically inhibited in the presence of heparin. Furthermore, under heparin administration, spindle shape formation was maintained but actin cytoskeleton was disrupted, resulting in loose cellular spreading. Conclusion: These results suggest that RGD type integrins and heparin-sensitive protein in coordination regulate cell morphology and proliferation on NANOZR, through the regulation of cell polarity and stress fiber formation, respectively. Keywords: zirconia, biocompatibility, adhesion, RGD-peptide, integrinsLuo FHong GMatsui HEndo KWan QSasaki KDove Medical PressarticlezirconiabiocompatibilityadhesionRGD peptideintegrinsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 7657-7667 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	zirconia biocompatibility adhesion RGD peptide integrins Medicine (General) R5-920
spellingShingle	zirconia biocompatibility adhesion RGD peptide integrins Medicine (General) R5-920 Luo F Hong G Matsui H Endo K Wan Q Sasaki K Initial osteoblast adhesion and subsequent differentiation on zirconia surfaces are regulated by integrins and heparin-sensitive molecule
description	Feng Luo,1,2 Guang Hong,3,4 Hiroyuki Matsui,2 Kosei Endo,5 Qianbing Wan,1 Keiichi Sasaki2 1State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China; 2Division of Advanced Prosthetic Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Japan; 3Liaison Center for Innovative Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Japan; 4Faculty of Dental Medicine, Airlangga University, Surabaya, Indonesia; 5Division of Aging and Geriatric Dentistry, Graduate School of Dentistry, Tohoku University, Sendai, Japan Purpose: It is well known that zirconia materials have good biocompatibility; however, little is known regarding the mechanism by which cells attach to these materials. The purpose of this study is to elucidate the mechanism of cell attachment. Materials and methods: In this study, we examined the surface characteristics of ceria-stabilized zirconia/alumina nanocomposite (NANOZR), yttria-stabilized zirconia (Y-TZP) and commercially pure titanium (CpTi), and we evaluated the initial response of osteoblast-like cells to them with different inhibitors. Results: Under the same polishing treatment, the three materials, NANOZR, Y-TZP and CpTi, show similar surface wettability but different surface roughness. Osteoblasts could adhere to the surface of all three materials, and spindle shapes were clearer in serum-containing media compared to PBS and serum-free culture media, suggesting that serum-contained proteins are helpful for the initial cell adhesion and spreading. Cell adhesion and proliferation were disrupted in the presence of EDTA. RGD-peptide interfered with cell proliferation by affecting cell protrusion and stress fibers. Monoclonal antibody against non-RGD type integrin α2β1 enhanced proliferation in Y-TZP, CpTi and culture dish but not in NANOZR. Cell proliferation on NANOZR was specifically inhibited in the presence of heparin. Furthermore, under heparin administration, spindle shape formation was maintained but actin cytoskeleton was disrupted, resulting in loose cellular spreading. Conclusion: These results suggest that RGD type integrins and heparin-sensitive protein in coordination regulate cell morphology and proliferation on NANOZR, through the regulation of cell polarity and stress fiber formation, respectively. Keywords: zirconia, biocompatibility, adhesion, RGD-peptide, integrins
format	article
author	Luo F Hong G Matsui H Endo K Wan Q Sasaki K
author_facet	Luo F Hong G Matsui H Endo K Wan Q Sasaki K
author_sort	Luo F
title	Initial osteoblast adhesion and subsequent differentiation on zirconia surfaces are regulated by integrins and heparin-sensitive molecule
title_short	Initial osteoblast adhesion and subsequent differentiation on zirconia surfaces are regulated by integrins and heparin-sensitive molecule
title_full	Initial osteoblast adhesion and subsequent differentiation on zirconia surfaces are regulated by integrins and heparin-sensitive molecule
title_fullStr	Initial osteoblast adhesion and subsequent differentiation on zirconia surfaces are regulated by integrins and heparin-sensitive molecule
title_full_unstemmed	Initial osteoblast adhesion and subsequent differentiation on zirconia surfaces are regulated by integrins and heparin-sensitive molecule
title_sort	initial osteoblast adhesion and subsequent differentiation on zirconia surfaces are regulated by integrins and heparin-sensitive molecule
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/e39485d095a341f4b587b90600da869c
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Initial osteoblast adhesion and subsequent differentiation on zirconia surfaces are regulated by integrins and heparin-sensitive molecule

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