Vitamin D deficiency promotes accumulation of bioactive lipids and increased endocannabinoid tone in zebrafish

Vitamin D is well known for its traditional role in bone mineral homeostasis; however, recent evidence suggests that vitamin D also plays a significant role in metabolic control. This study served to investigate putative linkages between vitamin D deficiency (VDD) and metabolic disruption of bioacti...

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Autores principales:	Megan M. Knuth, Whitney L. Stutts, Morgan M. Ritter, Kenneth P. Garrard, Seth W. Kullman
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	MS metabolomics lipidomics lipids nutrition endocannabinoid biosynthesis Biochemistry QD415-436
Acceso en línea:	https://doaj.org/article/e3a28bc75cff47f38e5ff3a3b680fa88
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spelling	oai:doaj.org-article:e3a28bc75cff47f38e5ff3a3b680fa882021-11-14T04:27:38ZVitamin D deficiency promotes accumulation of bioactive lipids and increased endocannabinoid tone in zebrafish0022-227510.1016/j.jlr.2021.100142https://doaj.org/article/e3a28bc75cff47f38e5ff3a3b680fa882021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0022227521001243https://doaj.org/toc/0022-2275Vitamin D is well known for its traditional role in bone mineral homeostasis; however, recent evidence suggests that vitamin D also plays a significant role in metabolic control. This study served to investigate putative linkages between vitamin D deficiency (VDD) and metabolic disruption of bioactive lipids by MS imaging. Our approach employed infrared-matrix-assisted laser desorption electrospray ionization MS imaging for lipid metabolite profiling in 6-month-old zebrafish fed either a VDD or a vitamin D-sufficient (VDS) diet. Using a lipidomics pipeline, we found that VDD zebrafish had a greater abundance of bioactive lipids (N-acyls, endocannabinoids [ECs], diacylglycerols/triacylglycerols, bile acids/bile alcohols, and vitamin D derivatives) suggestive of increased EC tone compared with VDS zebrafish. Tandem MS was performed on several differentially expressed metabolites with sufficient ion abundances to aid in structural elucidation and provide additional support for MS annotations. To confirm activation of the EC pathways, we subsequently examined expression of genes involved in EC biosynthesis, metabolism, and receptor signaling in adipose tissue and liver from VDD and VDS zebrafish. Gene expression changes were congruent with increased EC tone, with VDD zebrafish demonstrating increased synthesis and metabolism of anandamide compared with VDS zebrafish. Taken together, our data suggest that VDD may promote accumulation of bioactive lipids and increased EC tone in zebrafish.Megan M. KnuthWhitney L. StuttsMorgan M. RitterKenneth P. GarrardSeth W. KullmanElsevierarticleMSmetabolomicslipidomicslipidsnutritionendocannabinoid biosynthesisBiochemistryQD415-436ENJournal of Lipid Research, Vol 62, Iss , Pp 100142- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	MS metabolomics lipidomics lipids nutrition endocannabinoid biosynthesis Biochemistry QD415-436
spellingShingle	MS metabolomics lipidomics lipids nutrition endocannabinoid biosynthesis Biochemistry QD415-436 Megan M. Knuth Whitney L. Stutts Morgan M. Ritter Kenneth P. Garrard Seth W. Kullman Vitamin D deficiency promotes accumulation of bioactive lipids and increased endocannabinoid tone in zebrafish
description	Vitamin D is well known for its traditional role in bone mineral homeostasis; however, recent evidence suggests that vitamin D also plays a significant role in metabolic control. This study served to investigate putative linkages between vitamin D deficiency (VDD) and metabolic disruption of bioactive lipids by MS imaging. Our approach employed infrared-matrix-assisted laser desorption electrospray ionization MS imaging for lipid metabolite profiling in 6-month-old zebrafish fed either a VDD or a vitamin D-sufficient (VDS) diet. Using a lipidomics pipeline, we found that VDD zebrafish had a greater abundance of bioactive lipids (N-acyls, endocannabinoids [ECs], diacylglycerols/triacylglycerols, bile acids/bile alcohols, and vitamin D derivatives) suggestive of increased EC tone compared with VDS zebrafish. Tandem MS was performed on several differentially expressed metabolites with sufficient ion abundances to aid in structural elucidation and provide additional support for MS annotations. To confirm activation of the EC pathways, we subsequently examined expression of genes involved in EC biosynthesis, metabolism, and receptor signaling in adipose tissue and liver from VDD and VDS zebrafish. Gene expression changes were congruent with increased EC tone, with VDD zebrafish demonstrating increased synthesis and metabolism of anandamide compared with VDS zebrafish. Taken together, our data suggest that VDD may promote accumulation of bioactive lipids and increased EC tone in zebrafish.
format	article
author	Megan M. Knuth Whitney L. Stutts Morgan M. Ritter Kenneth P. Garrard Seth W. Kullman
author_facet	Megan M. Knuth Whitney L. Stutts Morgan M. Ritter Kenneth P. Garrard Seth W. Kullman
author_sort	Megan M. Knuth
title	Vitamin D deficiency promotes accumulation of bioactive lipids and increased endocannabinoid tone in zebrafish
title_short	Vitamin D deficiency promotes accumulation of bioactive lipids and increased endocannabinoid tone in zebrafish
title_full	Vitamin D deficiency promotes accumulation of bioactive lipids and increased endocannabinoid tone in zebrafish
title_fullStr	Vitamin D deficiency promotes accumulation of bioactive lipids and increased endocannabinoid tone in zebrafish
title_full_unstemmed	Vitamin D deficiency promotes accumulation of bioactive lipids and increased endocannabinoid tone in zebrafish
title_sort	vitamin d deficiency promotes accumulation of bioactive lipids and increased endocannabinoid tone in zebrafish
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e3a28bc75cff47f38e5ff3a3b680fa88
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Vitamin D deficiency promotes accumulation of bioactive lipids and increased endocannabinoid tone in zebrafish

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