Bioreducible and acid-labile poly(amido amine)s for efficient gene delivery

Zhi-Qiang Yu,1 Jun-Jie Yan,1 Ye-Zi You,1,* Qing-Hui Zhou2,*1Chinese Academy of Sciences, Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui, People&rsquo;s Republic of China; 2Department of Medic...

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Autores principales:	Zhou QH, You YZ, Yan JJ, Yu ZQ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e3aa2870ca5c4b1dac39d1a32bd15f03
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spelling	oai:doaj.org-article:e3aa2870ca5c4b1dac39d1a32bd15f032021-12-02T08:07:37ZBioreducible and acid-labile poly(amido amine)s for efficient gene delivery1176-91141178-2013https://doaj.org/article/e3aa2870ca5c4b1dac39d1a32bd15f032012-11-01T00:00:00Zhttp://www.dovepress.com/bioreducible-and-acid-labile-polyamido-amines-for-efficient-gene-deliv-a11594https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Zhi-Qiang Yu,1 Jun-Jie Yan,1 Ye-Zi You,1,* Qing-Hui Zhou2,1Chinese Academy of Sciences, Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui, People&rsquo;s Republic of China; 2Department of Medicine, University of California at Los Angeles, Los Angeles, CA, USAThese authors contributed equally to this workAbstract: Intracellular processes, including endosomal escape and intracellular release, are efficiency-determining steps in achieving successful gene delivery. It has been found that the presence of acid-labile units in polymers can facilitate endosomal escape and that the presence of reducible units in polymers can lead to intracellular release. In this study, poly(amido amine)s with both bioreducible and acid-labile properties were synthesized to improve gene delivery compared with single-responsive carriers. Transfection and cytotoxicity were evaluated in three cell lines. The complexes of DNA with dual-responsive polymers showed higher gene transfection efficiency than single-responsive polymers and polyethylenimine. At the same time, these polymers were tens of times less cytotoxic than polyethylenimine. Therefore, a polymer that is both reducible and acid-labile is a promising material for efficient and biocompatible gene delivery.Keywords: reducible, acid labile, gene delivery, dual-responsive, endosomal escape, intracellular releaseZhou QHYou YZYan JJYu ZQDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 5819-5832 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhou QH You YZ Yan JJ Yu ZQ Bioreducible and acid-labile poly(amido amine)s for efficient gene delivery
description	Zhi-Qiang Yu,1 Jun-Jie Yan,1 Ye-Zi You,1,* Qing-Hui Zhou2,1Chinese Academy of Sciences, Key Laboratory of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei, Anhui, People&rsquo;s Republic of China; 2Department of Medicine, University of California at Los Angeles, Los Angeles, CA, USAThese authors contributed equally to this workAbstract: Intracellular processes, including endosomal escape and intracellular release, are efficiency-determining steps in achieving successful gene delivery. It has been found that the presence of acid-labile units in polymers can facilitate endosomal escape and that the presence of reducible units in polymers can lead to intracellular release. In this study, poly(amido amine)s with both bioreducible and acid-labile properties were synthesized to improve gene delivery compared with single-responsive carriers. Transfection and cytotoxicity were evaluated in three cell lines. The complexes of DNA with dual-responsive polymers showed higher gene transfection efficiency than single-responsive polymers and polyethylenimine. At the same time, these polymers were tens of times less cytotoxic than polyethylenimine. Therefore, a polymer that is both reducible and acid-labile is a promising material for efficient and biocompatible gene delivery.Keywords: reducible, acid labile, gene delivery, dual-responsive, endosomal escape, intracellular release
format	article
author	Zhou QH You YZ Yan JJ Yu ZQ
author_facet	Zhou QH You YZ Yan JJ Yu ZQ
author_sort	Zhou QH
title	Bioreducible and acid-labile poly(amido amine)s for efficient gene delivery
title_short	Bioreducible and acid-labile poly(amido amine)s for efficient gene delivery
title_full	Bioreducible and acid-labile poly(amido amine)s for efficient gene delivery
title_fullStr	Bioreducible and acid-labile poly(amido amine)s for efficient gene delivery
title_full_unstemmed	Bioreducible and acid-labile poly(amido amine)s for efficient gene delivery
title_sort	bioreducible and acid-labile poly(amido amine)s for efficient gene delivery
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/e3aa2870ca5c4b1dac39d1a32bd15f03
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Bioreducible and acid-labile poly(amido amine)s for efficient gene delivery

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