MEKK5 Interacts with and Negatively Regulates the E3 Ubiquitin Ligase NEDD4 for Mediating Lung Cancer Cell Migration

Our previous studies have shown that the HECT E3 ubiquitin ligase NEDD4 and kinase MEKK5 both play an essential role in lung cancer migration. A report predicts that MEKK5 may be ubiquitinated by NEDD4; however, interaction of MEKK5 with NEDD4 and ubiquitination of MEKK5 by NEDD4 have not been chara...

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Autores principales:	Aiqin Sun, Jun Zhu, Song Xia, Yanling Li, Tiantian Wu, Genbao Shao, Wannian Yang, Qiong Lin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	MEKK5 NEDD4 E3 ubiquitin ligase ubiquitination cell migration Science Q
Acceso en línea:	https://doaj.org/article/e3ace83e789243b6a19e6afe8c11528f
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spelling	oai:doaj.org-article:e3ace83e789243b6a19e6afe8c11528f2021-11-25T18:10:43ZMEKK5 Interacts with and Negatively Regulates the E3 Ubiquitin Ligase NEDD4 for Mediating Lung Cancer Cell Migration10.3390/life111111532075-1729https://doaj.org/article/e3ace83e789243b6a19e6afe8c11528f2021-10-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1153https://doaj.org/toc/2075-1729Our previous studies have shown that the HECT E3 ubiquitin ligase NEDD4 and kinase MEKK5 both play an essential role in lung cancer migration. A report predicts that MEKK5 may be ubiquitinated by NEDD4; however, interaction of MEKK5 with NEDD4 and ubiquitination of MEKK5 by NEDD4 have not been characterized. In this report, we show that NEDD4 interacts with MEKK5 through a conserved WW3 domain by the co-immunoprecipitation and the GST-pulldown assays. The ubiquitination assay indicates that MEKK5 is not a ubiquitination substrate of NEDD4, but negatively regulates NEDD4-mediated ubiquitination. Furthermore, overexpression of MEKK5 significantly reduced the NEDD4-promoted lung cancer cell migration. Taken together, our studies have defined an inhibitory role of MEKK5 in regulation of NEDD4-mediated ubiquitination.Aiqin SunJun ZhuSong XiaYanling LiTiantian WuGenbao ShaoWannian YangQiong LinMDPI AGarticleMEKK5NEDD4E3 ubiquitin ligaseubiquitinationcell migrationScienceQENLife, Vol 11, Iss 1153, p 1153 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	MEKK5 NEDD4 E3 ubiquitin ligase ubiquitination cell migration Science Q
spellingShingle	MEKK5 NEDD4 E3 ubiquitin ligase ubiquitination cell migration Science Q Aiqin Sun Jun Zhu Song Xia Yanling Li Tiantian Wu Genbao Shao Wannian Yang Qiong Lin MEKK5 Interacts with and Negatively Regulates the E3 Ubiquitin Ligase NEDD4 for Mediating Lung Cancer Cell Migration
description	Our previous studies have shown that the HECT E3 ubiquitin ligase NEDD4 and kinase MEKK5 both play an essential role in lung cancer migration. A report predicts that MEKK5 may be ubiquitinated by NEDD4; however, interaction of MEKK5 with NEDD4 and ubiquitination of MEKK5 by NEDD4 have not been characterized. In this report, we show that NEDD4 interacts with MEKK5 through a conserved WW3 domain by the co-immunoprecipitation and the GST-pulldown assays. The ubiquitination assay indicates that MEKK5 is not a ubiquitination substrate of NEDD4, but negatively regulates NEDD4-mediated ubiquitination. Furthermore, overexpression of MEKK5 significantly reduced the NEDD4-promoted lung cancer cell migration. Taken together, our studies have defined an inhibitory role of MEKK5 in regulation of NEDD4-mediated ubiquitination.
format	article
author	Aiqin Sun Jun Zhu Song Xia Yanling Li Tiantian Wu Genbao Shao Wannian Yang Qiong Lin
author_facet	Aiqin Sun Jun Zhu Song Xia Yanling Li Tiantian Wu Genbao Shao Wannian Yang Qiong Lin
author_sort	Aiqin Sun
title	MEKK5 Interacts with and Negatively Regulates the E3 Ubiquitin Ligase NEDD4 for Mediating Lung Cancer Cell Migration
title_short	MEKK5 Interacts with and Negatively Regulates the E3 Ubiquitin Ligase NEDD4 for Mediating Lung Cancer Cell Migration
title_full	MEKK5 Interacts with and Negatively Regulates the E3 Ubiquitin Ligase NEDD4 for Mediating Lung Cancer Cell Migration
title_fullStr	MEKK5 Interacts with and Negatively Regulates the E3 Ubiquitin Ligase NEDD4 for Mediating Lung Cancer Cell Migration
title_full_unstemmed	MEKK5 Interacts with and Negatively Regulates the E3 Ubiquitin Ligase NEDD4 for Mediating Lung Cancer Cell Migration
title_sort	mekk5 interacts with and negatively regulates the e3 ubiquitin ligase nedd4 for mediating lung cancer cell migration
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e3ace83e789243b6a19e6afe8c11528f
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MEKK5 Interacts with and Negatively Regulates the E3 Ubiquitin Ligase NEDD4 for Mediating Lung Cancer Cell Migration

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