NAD-seq for profiling the NAD+ capped transcriptome of Arabidopsis thaliana
Summary: Eukaryotic RNAs can be modified with a non-canonical 5′ nicotinamide adenine dinucleotide (NAD+) cap. NAD-seq identifies transcriptome-wide NAD+ capped RNAs. NAD-seq takes advantage of click chemistry to allow the capture of NAD+ capped RNAs. Unlike other approaches, NAD-seq does not requir...
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| Main Authors: | , , |
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| Format: | article |
| Language: | EN |
| Published: |
Elsevier
2021
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| Subjects: | |
| Online Access: | https://doaj.org/article/e3b2951872f144749e381e62ce4ac686 |
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| Summary: | Summary: Eukaryotic RNAs can be modified with a non-canonical 5′ nicotinamide adenine dinucleotide (NAD+) cap. NAD-seq identifies transcriptome-wide NAD+ capped RNAs. NAD-seq takes advantage of click chemistry to allow the capture of NAD+ capped RNAs. Unlike other approaches, NAD-seq does not require DNA synthesis on beads, but this technique uses full NAD+ capped transcripts eluted from beads as the substrates for strand-specific RNA sequencing library preparation.For complete details on the use and execution of this protocol, please refer to Yu et al. (2021). |
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