NAD-seq for profiling the NAD+ capped transcriptome of Arabidopsis thaliana

Summary: Eukaryotic RNAs can be modified with a non-canonical 5′ nicotinamide adenine dinucleotide (NAD+) cap. NAD-seq identifies transcriptome-wide NAD+ capped RNAs. NAD-seq takes advantage of click chemistry to allow the capture of NAD+ capped RNAs. Unlike other approaches, NAD-seq does not requir...

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Autores principales:	Xiang Yu, Lee E. Vandivier, Brian D. Gregory
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Genomics RNAseq Model Organisms Plant sciences Molecular Biology Gene Expression Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/e3b2951872f144749e381e62ce4ac686
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spelling	oai:doaj.org-article:e3b2951872f144749e381e62ce4ac6862021-11-14T04:35:35ZNAD-seq for profiling the NAD+ capped transcriptome of Arabidopsis thaliana2666-166710.1016/j.xpro.2021.100901https://doaj.org/article/e3b2951872f144749e381e62ce4ac6862021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006079https://doaj.org/toc/2666-1667Summary: Eukaryotic RNAs can be modified with a non-canonical 5′ nicotinamide adenine dinucleotide (NAD+) cap. NAD-seq identifies transcriptome-wide NAD+ capped RNAs. NAD-seq takes advantage of click chemistry to allow the capture of NAD+ capped RNAs. Unlike other approaches, NAD-seq does not require DNA synthesis on beads, but this technique uses full NAD+ capped transcripts eluted from beads as the substrates for strand-specific RNA sequencing library preparation.For complete details on the use and execution of this protocol, please refer to Yu et al. (2021).Xiang YuLee E. VandivierBrian D. GregoryElsevierarticleGenomicsRNAseqModel OrganismsPlant sciencesMolecular BiologyGene ExpressionScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100901- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genomics RNAseq Model Organisms Plant sciences Molecular Biology Gene Expression Science (General) Q1-390
spellingShingle	Genomics RNAseq Model Organisms Plant sciences Molecular Biology Gene Expression Science (General) Q1-390 Xiang Yu Lee E. Vandivier Brian D. Gregory NAD-seq for profiling the NAD+ capped transcriptome of Arabidopsis thaliana
description	Summary: Eukaryotic RNAs can be modified with a non-canonical 5′ nicotinamide adenine dinucleotide (NAD+) cap. NAD-seq identifies transcriptome-wide NAD+ capped RNAs. NAD-seq takes advantage of click chemistry to allow the capture of NAD+ capped RNAs. Unlike other approaches, NAD-seq does not require DNA synthesis on beads, but this technique uses full NAD+ capped transcripts eluted from beads as the substrates for strand-specific RNA sequencing library preparation.For complete details on the use and execution of this protocol, please refer to Yu et al. (2021).
format	article
author	Xiang Yu Lee E. Vandivier Brian D. Gregory
author_facet	Xiang Yu Lee E. Vandivier Brian D. Gregory
author_sort	Xiang Yu
title	NAD-seq for profiling the NAD+ capped transcriptome of Arabidopsis thaliana
title_short	NAD-seq for profiling the NAD+ capped transcriptome of Arabidopsis thaliana
title_full	NAD-seq for profiling the NAD+ capped transcriptome of Arabidopsis thaliana
title_fullStr	NAD-seq for profiling the NAD+ capped transcriptome of Arabidopsis thaliana
title_full_unstemmed	NAD-seq for profiling the NAD+ capped transcriptome of Arabidopsis thaliana
title_sort	nad-seq for profiling the nad+ capped transcriptome of arabidopsis thaliana
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e3b2951872f144749e381e62ce4ac686
work_keys_str_mv	AT xiangyu nadseqforprofilingthenadcappedtranscriptomeofarabidopsisthaliana AT leeevandivier nadseqforprofilingthenadcappedtranscriptomeofarabidopsisthaliana AT briandgregory nadseqforprofilingthenadcappedtranscriptomeofarabidopsisthaliana
_version_	1718429929750659072

NAD-seq for profiling the NAD+ capped transcriptome of Arabidopsis thaliana

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