Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications

Margarita Kurochkina,1 Elena Konshina,1 Aleksandr Oseev,2 Soeren Hirsch3 1Centre of Information Optical Technologies, ITMO University, Saint Petersburg, Russia; 2Institute of Micro and Sensor Systems, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany; 3Department of Engineering, University...

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Autores principales:	Kurochkina M, Konshina E, Oseev A, Hirsch S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	quantum dots hybrid structures photoluminescence exciton-plasmon interaction optical biosensor proteins Medical technology R855-855.5 Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/e3b2f50c1f104d27acdbf37b7b017196
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spelling	oai:doaj.org-article:e3b2f50c1f104d27acdbf37b7b0171962021-12-02T01:13:04ZHybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications1177-8903https://doaj.org/article/e3b2f50c1f104d27acdbf37b7b0171962018-04-01T00:00:00Zhttps://www.dovepress.com/hybrid-structures-based-on-gold-nanoparticles-and-semiconductor-quantu-peer-reviewed-article-NSAhttps://doaj.org/toc/1177-8903Margarita Kurochkina,1 Elena Konshina,1 Aleksandr Oseev,2 Soeren Hirsch3 1Centre of Information Optical Technologies, ITMO University, Saint Petersburg, Russia; 2Institute of Micro and Sensor Systems, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany; 3Department of Engineering, University of Applied Sciences Brandenburg, Brandenburg an der Havel, Germany Background: The luminescence amplification of semiconductor quantum dots (QD) in the presence of self-assembled gold nanoparticles (Au NPs) is one of way for creating biosensors with highly efficient transduction. Aims: The objective of this study was to fabricate the hybrid structures based on semiconductor CdSe/ZnS QDs and Au NP arrays and to use them as biosensors of protein. Methods: In this paper, the hybrid structures based on CdSe/ZnS QDs and Au NP arrays were fabricated using spin coating processes. Au NP arrays deposited on a glass wafer were investigated by optical microscopy and absorption spectroscopy depending on numbers of spin coating layers and their baking temperature. Bovine serum albumin (BSA) was used as the target protein analyte in a phosphate buffer. A confocal laser scanning microscope was used to study the luminescent properties of Au NP/QD hybrid structures and to test BSA. Results: The dimensions of Au NP aggregates increased and the space between them decreased with increasing processing temperature. At the same time, a blue shift of the plasmon resonance peak in the absorption spectra of Au NP arrays was observed. The deposition of CdSe/ZnS QDs with a core diameter of 5 nm on the surface of the Au NP arrays caused an increase in absorption and a red shift of the plasmon peak in the spectra. The exciton–plasmon enhancement of the QDs’ photoluminescence intensity has been obtained at room temperature for hybrid structures with Au NPs array pretreated at temperatures of 100°C and 150°C. It has been found that an increase in the weight content of BSA increases the photoluminescence intensity of such hybrid structures. Conclusion: The ability of the qualitative and quantitative determination of protein content in solution using the Au NP/QD structures as an optical biosensor has been shown experimentally. Keywords: quantum dots, hybrid structures, photoluminescence, exciton–plasmon interaction, optical biosensor, proteinsKurochkina MKonshina EOseev AHirsch SDove Medical Pressarticlequantum dotshybrid structuresphotoluminescenceexciton-plasmon interactionoptical biosensorproteinsMedical technologyR855-855.5Chemical technologyTP1-1185ENNanotechnology, Science and Applications, Vol Volume 11, Pp 15-21 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	quantum dots hybrid structures photoluminescence exciton-plasmon interaction optical biosensor proteins Medical technology R855-855.5 Chemical technology TP1-1185
spellingShingle	quantum dots hybrid structures photoluminescence exciton-plasmon interaction optical biosensor proteins Medical technology R855-855.5 Chemical technology TP1-1185 Kurochkina M Konshina E Oseev A Hirsch S Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications
description	Margarita Kurochkina,1 Elena Konshina,1 Aleksandr Oseev,2 Soeren Hirsch3 1Centre of Information Optical Technologies, ITMO University, Saint Petersburg, Russia; 2Institute of Micro and Sensor Systems, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany; 3Department of Engineering, University of Applied Sciences Brandenburg, Brandenburg an der Havel, Germany Background: The luminescence amplification of semiconductor quantum dots (QD) in the presence of self-assembled gold nanoparticles (Au NPs) is one of way for creating biosensors with highly efficient transduction. Aims: The objective of this study was to fabricate the hybrid structures based on semiconductor CdSe/ZnS QDs and Au NP arrays and to use them as biosensors of protein. Methods: In this paper, the hybrid structures based on CdSe/ZnS QDs and Au NP arrays were fabricated using spin coating processes. Au NP arrays deposited on a glass wafer were investigated by optical microscopy and absorption spectroscopy depending on numbers of spin coating layers and their baking temperature. Bovine serum albumin (BSA) was used as the target protein analyte in a phosphate buffer. A confocal laser scanning microscope was used to study the luminescent properties of Au NP/QD hybrid structures and to test BSA. Results: The dimensions of Au NP aggregates increased and the space between them decreased with increasing processing temperature. At the same time, a blue shift of the plasmon resonance peak in the absorption spectra of Au NP arrays was observed. The deposition of CdSe/ZnS QDs with a core diameter of 5 nm on the surface of the Au NP arrays caused an increase in absorption and a red shift of the plasmon peak in the spectra. The exciton–plasmon enhancement of the QDs’ photoluminescence intensity has been obtained at room temperature for hybrid structures with Au NPs array pretreated at temperatures of 100°C and 150°C. It has been found that an increase in the weight content of BSA increases the photoluminescence intensity of such hybrid structures. Conclusion: The ability of the qualitative and quantitative determination of protein content in solution using the Au NP/QD structures as an optical biosensor has been shown experimentally. Keywords: quantum dots, hybrid structures, photoluminescence, exciton–plasmon interaction, optical biosensor, proteins
format	article
author	Kurochkina M Konshina E Oseev A Hirsch S
author_facet	Kurochkina M Konshina E Oseev A Hirsch S
author_sort	Kurochkina M
title	Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications
title_short	Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications
title_full	Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications
title_fullStr	Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications
title_full_unstemmed	Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications
title_sort	hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/e3b2f50c1f104d27acdbf37b7b017196
work_keys_str_mv	AT kurochkinam hybridstructuresbasedongoldnanoparticlesandsemiconductorquantumdotsforbiosensorapplications AT konshinae hybridstructuresbasedongoldnanoparticlesandsemiconductorquantumdotsforbiosensorapplications AT oseeva hybridstructuresbasedongoldnanoparticlesandsemiconductorquantumdotsforbiosensorapplications AT hirschs hybridstructuresbasedongoldnanoparticlesandsemiconductorquantumdotsforbiosensorapplications
_version_	1718403228713877504

Hybrid structures based on gold nanoparticles and semiconductor quantum dots for biosensor applications

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