The relative effects of Ca and Mg ions on MSC osteogenesis in the surface modification of microrough Ti implants

Jin-Woo Park,1 Takao Hanawa,2 Jong-Hyuk Chung31Department of Periodontology, School of Dentistry, Kyungpook National University, Daegu 41940, Republic of Korea; 2Department of Metallic Biomaterials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo 101-0062, Ja...

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Autores principales:	Park JW, Hanawa T, Chung JH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	divalent cations surface chemistry nanotopography bone implant Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e3b6dedd6f7b4b208fbec63746481f71
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spelling	oai:doaj.org-article:e3b6dedd6f7b4b208fbec63746481f712021-12-02T08:07:07ZThe relative effects of Ca and Mg ions on MSC osteogenesis in the surface modification of microrough Ti implants1178-2013https://doaj.org/article/e3b6dedd6f7b4b208fbec63746481f712019-07-01T00:00:00Zhttps://www.dovepress.com/the-relative-effects-of-ca-and-mg-ions-on-msc-osteogenesis-in-the-surf-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jin-Woo Park,1 Takao Hanawa,2 Jong-Hyuk Chung31Department of Periodontology, School of Dentistry, Kyungpook National University, Daegu 41940, Republic of Korea; 2Department of Metallic Biomaterials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo 101-0062, Japan; 3Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Republic of KoreaPurpose: Calcium (Ca) and magnesium (Mg) ions have been used as promising bioactive ions in the surface chemistry modification of titanium (Ti) bone implants to increase bone regeneration capacity. However, it is not clear which (Ca or Mg) plays the more important role in the early osteogenic differentiation of mesenchymal stem cells (MSCs) when applied to the surface of commercially available microstructured Ti implants. This study investigated the relative effect of these two ions on the early osteogenic functionality of primary mouse bone marrow MSCs in order to obtain insights into the surface design of Ti implants with enhanced early osteogenic capacity.Methods and results: Wet chemical treatment was performed to modify a microrough Ti implant surface using Ca or Mg ions. Both the Ca and Mg-incorporated surfaces accelerated early cellular events and the subsequent osteogenic differentiation of MSCs compared with an unmodified microrough Ti surface. Surface Mg modification exhibited a more potent osteoblast differentiation-promoting effect than the Ca modification. Surface Mg incorporation markedly inhibited the phosphorylation of β-catenin.Conclusion: These results indicate that alteration of the surface chemistry of microstructured Ti implants by wet chemical treatment with Mg ions exerts a more effect on promoting the early osteogenic differentiation of MSCs than Ca ions by enhancing early cellular functions, including focal adhesion development and stabilization of intracellular β-catenin.Keywords: divalent cations, surface chemistry, nanotopography, bone implantPark JWHanawa TChung JHDove Medical Pressarticledivalent cationssurface chemistrynanotopographybone implantMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 5697-5711 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	divalent cations surface chemistry nanotopography bone implant Medicine (General) R5-920
spellingShingle	divalent cations surface chemistry nanotopography bone implant Medicine (General) R5-920 Park JW Hanawa T Chung JH The relative effects of Ca and Mg ions on MSC osteogenesis in the surface modification of microrough Ti implants
description	Jin-Woo Park,1 Takao Hanawa,2 Jong-Hyuk Chung31Department of Periodontology, School of Dentistry, Kyungpook National University, Daegu 41940, Republic of Korea; 2Department of Metallic Biomaterials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo 101-0062, Japan; 3Department of Periodontology, School of Dentistry, Kyung Hee University, Seoul, Republic of KoreaPurpose: Calcium (Ca) and magnesium (Mg) ions have been used as promising bioactive ions in the surface chemistry modification of titanium (Ti) bone implants to increase bone regeneration capacity. However, it is not clear which (Ca or Mg) plays the more important role in the early osteogenic differentiation of mesenchymal stem cells (MSCs) when applied to the surface of commercially available microstructured Ti implants. This study investigated the relative effect of these two ions on the early osteogenic functionality of primary mouse bone marrow MSCs in order to obtain insights into the surface design of Ti implants with enhanced early osteogenic capacity.Methods and results: Wet chemical treatment was performed to modify a microrough Ti implant surface using Ca or Mg ions. Both the Ca and Mg-incorporated surfaces accelerated early cellular events and the subsequent osteogenic differentiation of MSCs compared with an unmodified microrough Ti surface. Surface Mg modification exhibited a more potent osteoblast differentiation-promoting effect than the Ca modification. Surface Mg incorporation markedly inhibited the phosphorylation of β-catenin.Conclusion: These results indicate that alteration of the surface chemistry of microstructured Ti implants by wet chemical treatment with Mg ions exerts a more effect on promoting the early osteogenic differentiation of MSCs than Ca ions by enhancing early cellular functions, including focal adhesion development and stabilization of intracellular β-catenin.Keywords: divalent cations, surface chemistry, nanotopography, bone implant
format	article
author	Park JW Hanawa T Chung JH
author_facet	Park JW Hanawa T Chung JH
author_sort	Park JW
title	The relative effects of Ca and Mg ions on MSC osteogenesis in the surface modification of microrough Ti implants
title_short	The relative effects of Ca and Mg ions on MSC osteogenesis in the surface modification of microrough Ti implants
title_full	The relative effects of Ca and Mg ions on MSC osteogenesis in the surface modification of microrough Ti implants
title_fullStr	The relative effects of Ca and Mg ions on MSC osteogenesis in the surface modification of microrough Ti implants
title_full_unstemmed	The relative effects of Ca and Mg ions on MSC osteogenesis in the surface modification of microrough Ti implants
title_sort	relative effects of ca and mg ions on msc osteogenesis in the surface modification of microrough ti implants
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/e3b6dedd6f7b4b208fbec63746481f71
work_keys_str_mv	AT parkjw therelativeeffectsofcaandmgionsonmscosteogenesisinthesurfacemodificationofmicroroughtiimplants AT hanawat therelativeeffectsofcaandmgionsonmscosteogenesisinthesurfacemodificationofmicroroughtiimplants AT chungjh therelativeeffectsofcaandmgionsonmscosteogenesisinthesurfacemodificationofmicroroughtiimplants AT parkjw relativeeffectsofcaandmgionsonmscosteogenesisinthesurfacemodificationofmicroroughtiimplants AT hanawat relativeeffectsofcaandmgionsonmscosteogenesisinthesurfacemodificationofmicroroughtiimplants AT chungjh relativeeffectsofcaandmgionsonmscosteogenesisinthesurfacemodificationofmicroroughtiimplants
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The relative effects of Ca and Mg ions on MSC osteogenesis in the surface modification of microrough Ti implants

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