Sequence investigation of 34 forensic autosomal STRs with massively parallel sequencing

Abstract STRs vary not only in the length of the repeat units and the number of repeats but also in the region with which they conform to an incremental repeat pattern. Massively parallel sequencing (MPS) offers new possibilities in the analysis of STRs since they can simultaneously sequence multipl...

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Autores principales:	Suhua Zhang, Yong Niu, Yingnan Bian, Rixia Dong, Xiling Liu, Yun Bao, Chao Jin, Hancheng Zheng, Chengtao Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e3b6f079b5d143109132cda75d39f337
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spelling	oai:doaj.org-article:e3b6f079b5d143109132cda75d39f3372021-12-02T16:08:15ZSequence investigation of 34 forensic autosomal STRs with massively parallel sequencing10.1038/s41598-018-24495-92045-2322https://doaj.org/article/e3b6f079b5d143109132cda75d39f3372018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24495-9https://doaj.org/toc/2045-2322Abstract STRs vary not only in the length of the repeat units and the number of repeats but also in the region with which they conform to an incremental repeat pattern. Massively parallel sequencing (MPS) offers new possibilities in the analysis of STRs since they can simultaneously sequence multiple targets in a single reaction and capture potential internal sequence variations. Here, we sequenced 34 STRs applied in the forensic community of China with a custom-designed panel. MPS performance were evaluated from sequencing reads analysis, concordance study and sensitivity testing. High coverage sequencing data were obtained to determine the constitute ratios and heterozygous balance. No actual inconsistent genotypes were observed between capillary electrophoresis (CE) and MPS, demonstrating the reliability of the panel and the MPS technology. With the sequencing data from the 200 investigated individuals, 346 and 418 alleles were obtained via CE and MPS technologies at the 34 STRs, indicating MPS technology provides higher discrimination than CE detection. The whole study demonstrated that STR genotyping with the custom panel and MPS technology has the potential not only to reveal length and sequence variations but also to satisfy the demands of high throughput and high multiplexing with acceptable sensitivity.Suhua ZhangYong NiuYingnan BianRixia DongXiling LiuYun BaoChao JinHancheng ZhengChengtao LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-7 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Suhua Zhang Yong Niu Yingnan Bian Rixia Dong Xiling Liu Yun Bao Chao Jin Hancheng Zheng Chengtao Li Sequence investigation of 34 forensic autosomal STRs with massively parallel sequencing
description	Abstract STRs vary not only in the length of the repeat units and the number of repeats but also in the region with which they conform to an incremental repeat pattern. Massively parallel sequencing (MPS) offers new possibilities in the analysis of STRs since they can simultaneously sequence multiple targets in a single reaction and capture potential internal sequence variations. Here, we sequenced 34 STRs applied in the forensic community of China with a custom-designed panel. MPS performance were evaluated from sequencing reads analysis, concordance study and sensitivity testing. High coverage sequencing data were obtained to determine the constitute ratios and heterozygous balance. No actual inconsistent genotypes were observed between capillary electrophoresis (CE) and MPS, demonstrating the reliability of the panel and the MPS technology. With the sequencing data from the 200 investigated individuals, 346 and 418 alleles were obtained via CE and MPS technologies at the 34 STRs, indicating MPS technology provides higher discrimination than CE detection. The whole study demonstrated that STR genotyping with the custom panel and MPS technology has the potential not only to reveal length and sequence variations but also to satisfy the demands of high throughput and high multiplexing with acceptable sensitivity.
format	article
author	Suhua Zhang Yong Niu Yingnan Bian Rixia Dong Xiling Liu Yun Bao Chao Jin Hancheng Zheng Chengtao Li
author_facet	Suhua Zhang Yong Niu Yingnan Bian Rixia Dong Xiling Liu Yun Bao Chao Jin Hancheng Zheng Chengtao Li
author_sort	Suhua Zhang
title	Sequence investigation of 34 forensic autosomal STRs with massively parallel sequencing
title_short	Sequence investigation of 34 forensic autosomal STRs with massively parallel sequencing
title_full	Sequence investigation of 34 forensic autosomal STRs with massively parallel sequencing
title_fullStr	Sequence investigation of 34 forensic autosomal STRs with massively parallel sequencing
title_full_unstemmed	Sequence investigation of 34 forensic autosomal STRs with massively parallel sequencing
title_sort	sequence investigation of 34 forensic autosomal strs with massively parallel sequencing
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/e3b6f079b5d143109132cda75d39f337
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_version_	1718384565614018560

Sequence investigation of 34 forensic autosomal STRs with massively parallel sequencing

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