Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources.

CRP (cAMP receptor protein), the global regulator of genes for carbon source utilization in the absence of glucose, is the best-studied prokaryotic transcription factor. A total of 195 target promoters on the Escherichia coli genome have been proposed to be under the control of cAMP-bound CRP. Using...

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Autores principales:	Tomohiro Shimada, Nobuyuki Fujita, Kaneyoshi Yamamoto, Akira Ishihama
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e3cd8e2ed7bb4d40a00285ddf5b38059
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spelling	oai:doaj.org-article:e3cd8e2ed7bb4d40a00285ddf5b380592021-11-18T06:52:46ZNovel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources.1932-620310.1371/journal.pone.0020081https://doaj.org/article/e3cd8e2ed7bb4d40a00285ddf5b380592011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21673794/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203CRP (cAMP receptor protein), the global regulator of genes for carbon source utilization in the absence of glucose, is the best-studied prokaryotic transcription factor. A total of 195 target promoters on the Escherichia coli genome have been proposed to be under the control of cAMP-bound CRP. Using the newly developed Genomic SELEX screening system of transcription factor-binding sequences, however, we have identified a total of at least 254 CRP-binding sites. Based on their location on the E. coli genome, we predict a total of at least 183 novel regulation target operons, altogether with the 195 hitherto known targets, reaching to the minimum of 378 promoters as the regulation targets of cAMP-CRP. All the promoters selected from the newly identified targets and examined by using the lacZ reporter assay were found to be under the control of CRP, indicating that the Genomic SELEX screening allowed to identify the CRP targets with high accuracy. Based on the functions of novel target genes, we conclude that CRP plays a key regulatory role in the whole processes from the selective transport of carbon sources, the glycolysis-gluconeogenesis switching to the metabolisms downstream of glycolysis, including tricarboxylic acid (TCA) cycle, pyruvate dehydrogenase (PDH) pathway and aerobic respiration. One unique regulation mode is that a single and the same CRP molecule bound within intergenic regions often regulates both of divergently transcribed operons.Tomohiro ShimadaNobuyuki FujitaKaneyoshi YamamotoAkira IshihamaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 6, p e20081 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tomohiro Shimada Nobuyuki Fujita Kaneyoshi Yamamoto Akira Ishihama Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources.
description	CRP (cAMP receptor protein), the global regulator of genes for carbon source utilization in the absence of glucose, is the best-studied prokaryotic transcription factor. A total of 195 target promoters on the Escherichia coli genome have been proposed to be under the control of cAMP-bound CRP. Using the newly developed Genomic SELEX screening system of transcription factor-binding sequences, however, we have identified a total of at least 254 CRP-binding sites. Based on their location on the E. coli genome, we predict a total of at least 183 novel regulation target operons, altogether with the 195 hitherto known targets, reaching to the minimum of 378 promoters as the regulation targets of cAMP-CRP. All the promoters selected from the newly identified targets and examined by using the lacZ reporter assay were found to be under the control of CRP, indicating that the Genomic SELEX screening allowed to identify the CRP targets with high accuracy. Based on the functions of novel target genes, we conclude that CRP plays a key regulatory role in the whole processes from the selective transport of carbon sources, the glycolysis-gluconeogenesis switching to the metabolisms downstream of glycolysis, including tricarboxylic acid (TCA) cycle, pyruvate dehydrogenase (PDH) pathway and aerobic respiration. One unique regulation mode is that a single and the same CRP molecule bound within intergenic regions often regulates both of divergently transcribed operons.
format	article
author	Tomohiro Shimada Nobuyuki Fujita Kaneyoshi Yamamoto Akira Ishihama
author_facet	Tomohiro Shimada Nobuyuki Fujita Kaneyoshi Yamamoto Akira Ishihama
author_sort	Tomohiro Shimada
title	Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources.
title_short	Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources.
title_full	Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources.
title_fullStr	Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources.
title_full_unstemmed	Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources.
title_sort	novel roles of camp receptor protein (crp) in regulation of transport and metabolism of carbon sources.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/e3cd8e2ed7bb4d40a00285ddf5b38059
work_keys_str_mv	AT tomohiroshimada novelrolesofcampreceptorproteincrpinregulationoftransportandmetabolismofcarbonsources AT nobuyukifujita novelrolesofcampreceptorproteincrpinregulationoftransportandmetabolismofcarbonsources AT kaneyoshiyamamoto novelrolesofcampreceptorproteincrpinregulationoftransportandmetabolismofcarbonsources AT akiraishihama novelrolesofcampreceptorproteincrpinregulationoftransportandmetabolismofcarbonsources
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Novel roles of cAMP receptor protein (CRP) in regulation of transport and metabolism of carbon sources.

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