TAT-LHRH conjugated low molecular weight chitosan as a gene carrier specific for hepatocellular carcinoma cells

Lanxia Liu, Xia Dong, Dunwan Zhu, Liping Song, Hailing Zhang, Xigang G LengLaboratory of Bioengineering, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin Key Laboratory of Biomedical Materials, Tianjin, People’s Republic of...

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Autores principales:	Liu LX, Dong X, Zhu DW, Song LP, Zhang HL, Leng XG
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e3df31da60ec45c49443068c82f1101d
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spelling	oai:doaj.org-article:e3df31da60ec45c49443068c82f1101d2021-12-02T05:57:52ZTAT-LHRH conjugated low molecular weight chitosan as a gene carrier specific for hepatocellular carcinoma cells1178-2013https://doaj.org/article/e3df31da60ec45c49443068c82f1101d2014-06-01T00:00:00Zhttp://www.dovepress.com/tat-lhrh-conjugated-low-molecular-weight-chitosan-as-a-gene-carrier-sp-a17151https://doaj.org/toc/1178-2013 Lanxia Liu, Xia Dong, Dunwan Zhu, Liping Song, Hailing Zhang, Xigang G LengLaboratory of Bioengineering, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin Key Laboratory of Biomedical Materials, Tianjin, People’s Republic of ChinaAbstract: To develop a chitosan-based nonviral gene carrier capable of delivering genes specifically into hepatoma cells, a bifunctional peptide composed of the TAT (transactivator of transcription) peptide and luteinizing hormone-releasing hormone (LHRH) was conjugated with low molecular weight chitosan, resulting in a TAT-LHRH-chitosan conjugate (TLC). TLC/DNA nanoparticles (TLCDNPs) were characterized by agarose gel retardation, atomic force microscopy, and dynamic light scattering analysis. In vitro targeting specificity and transfection efficiency were analyzed with a GE IN Cell Analyzer 2000 High-Content Cellular Analysis System. The results demonstrated that TLC had stronger DNA condensing power than unmodified chitosan, and that TLCDNPs were of roughly round shape with average diameter of 70–85 nm and zeta potential of +30 mV and were relatively stable in solution. The in vitro study demonstrated TLC was highly selective for hepatoma cells and essentially nontoxic.Keywords: nanoparticles, cancer treatment, gene therapy, hepatoma, targeted gene deliveryLiu LXDong XZhu DWSong LPZhang HLLeng XGDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 2879-2889 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Liu LX Dong X Zhu DW Song LP Zhang HL Leng XG TAT-LHRH conjugated low molecular weight chitosan as a gene carrier specific for hepatocellular carcinoma cells
description	Lanxia Liu, Xia Dong, Dunwan Zhu, Liping Song, Hailing Zhang, Xigang G LengLaboratory of Bioengineering, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin Key Laboratory of Biomedical Materials, Tianjin, People’s Republic of ChinaAbstract: To develop a chitosan-based nonviral gene carrier capable of delivering genes specifically into hepatoma cells, a bifunctional peptide composed of the TAT (transactivator of transcription) peptide and luteinizing hormone-releasing hormone (LHRH) was conjugated with low molecular weight chitosan, resulting in a TAT-LHRH-chitosan conjugate (TLC). TLC/DNA nanoparticles (TLCDNPs) were characterized by agarose gel retardation, atomic force microscopy, and dynamic light scattering analysis. In vitro targeting specificity and transfection efficiency were analyzed with a GE IN Cell Analyzer 2000 High-Content Cellular Analysis System. The results demonstrated that TLC had stronger DNA condensing power than unmodified chitosan, and that TLCDNPs were of roughly round shape with average diameter of 70–85 nm and zeta potential of +30 mV and were relatively stable in solution. The in vitro study demonstrated TLC was highly selective for hepatoma cells and essentially nontoxic.Keywords: nanoparticles, cancer treatment, gene therapy, hepatoma, targeted gene delivery
format	article
author	Liu LX Dong X Zhu DW Song LP Zhang HL Leng XG
author_facet	Liu LX Dong X Zhu DW Song LP Zhang HL Leng XG
author_sort	Liu LX
title	TAT-LHRH conjugated low molecular weight chitosan as a gene carrier specific for hepatocellular carcinoma cells
title_short	TAT-LHRH conjugated low molecular weight chitosan as a gene carrier specific for hepatocellular carcinoma cells
title_full	TAT-LHRH conjugated low molecular weight chitosan as a gene carrier specific for hepatocellular carcinoma cells
title_fullStr	TAT-LHRH conjugated low molecular weight chitosan as a gene carrier specific for hepatocellular carcinoma cells
title_full_unstemmed	TAT-LHRH conjugated low molecular weight chitosan as a gene carrier specific for hepatocellular carcinoma cells
title_sort	tat-lhrh conjugated low molecular weight chitosan as a gene carrier specific for hepatocellular carcinoma cells
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/e3df31da60ec45c49443068c82f1101d
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TAT-LHRH conjugated low molecular weight chitosan as a gene carrier specific for hepatocellular carcinoma cells

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