Enhancing chondrogenic potential via mesenchymal stem cell sheet multilayering

Advanced tissue engineering approaches for direct articular cartilage replacement in vivo employ mesenchymal stem cell (MSC) sources, exploiting innate chondrogenic potential to fabricate hyaline-like constructs in vitro within three-dimensional (3D) culture conditions. Cell sheet technology represe...

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Autores principales:	Hallie Thorp, Kyungsook Kim, Sophia Bou-Ghannam, Makoto Kondo, Travis Maak, David W. Grainger, Teruo Okano
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Tissue engineering Chondrogenic differentiation Scaffold-free Cellular interactions Cell sheet technology Medicine (General) R5-920 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/e3e2a85b4c104a94a2a41d7f88aab775
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spelling	oai:doaj.org-article:e3e2a85b4c104a94a2a41d7f88aab7752021-12-04T04:34:36ZEnhancing chondrogenic potential via mesenchymal stem cell sheet multilayering2352-320410.1016/j.reth.2021.11.004https://doaj.org/article/e3e2a85b4c104a94a2a41d7f88aab7752021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352320421000869https://doaj.org/toc/2352-3204Advanced tissue engineering approaches for direct articular cartilage replacement in vivo employ mesenchymal stem cell (MSC) sources, exploiting innate chondrogenic potential to fabricate hyaline-like constructs in vitro within three-dimensional (3D) culture conditions. Cell sheet technology represents one such advanced 3D scaffold-free cell culture platform, and previous work has shown that 3D MSC sheets are capable of in vitro hyaline-like chondrogenic differentiation. The present study aims to build upon this understanding and elucidate the effects of an established cell sheet manipulation technique, cell sheet multilayering, on fabrication of MSC-derived hyaline-like cartilage 3D layered constructs in vitro. To achieve this goal, multilayered MSC sheets are prepared and assessed for structural and biochemical transitions throughout chondrogenesis. Results support MSC multilayering as a means of increasing construct thickness and 3D cellular interactions related to in vitro chondrogenesis, including N-cadherin, connexin 43, and integrin β-1. Data indicate that increasing construct thickness from 14 μm (1-layer construct) to 25 μm (2-layer construct) increases these cellular interactions and subsequent in vitro MSC chondrogenesis. However, a clear initial thickness threshold (33 μm - 3-layer construct) is evident that decreases the rate and extent of in vitro chondrogenesis, specifically chondrogenic gene expressions (Sox9, aggrecan, type II collagen) and sulfated proteoglycan accumulation in deposited extracellular matrix (ECM). Together, these data support the utility of cell sheet multilayering as a platform for tailoring construct thickness and subsequent MSC chondrogenesis for future articular cartilage regeneration applications.Hallie ThorpKyungsook KimSophia Bou-GhannamMakoto KondoTravis MaakDavid W. GraingerTeruo OkanoElsevierarticleTissue engineeringChondrogenic differentiationScaffold-freeCellular interactionsCell sheet technologyMedicine (General)R5-920CytologyQH573-671ENRegenerative Therapy, Vol 18, Iss , Pp 487-496 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Tissue engineering Chondrogenic differentiation Scaffold-free Cellular interactions Cell sheet technology Medicine (General) R5-920 Cytology QH573-671
spellingShingle	Tissue engineering Chondrogenic differentiation Scaffold-free Cellular interactions Cell sheet technology Medicine (General) R5-920 Cytology QH573-671 Hallie Thorp Kyungsook Kim Sophia Bou-Ghannam Makoto Kondo Travis Maak David W. Grainger Teruo Okano Enhancing chondrogenic potential via mesenchymal stem cell sheet multilayering
description	Advanced tissue engineering approaches for direct articular cartilage replacement in vivo employ mesenchymal stem cell (MSC) sources, exploiting innate chondrogenic potential to fabricate hyaline-like constructs in vitro within three-dimensional (3D) culture conditions. Cell sheet technology represents one such advanced 3D scaffold-free cell culture platform, and previous work has shown that 3D MSC sheets are capable of in vitro hyaline-like chondrogenic differentiation. The present study aims to build upon this understanding and elucidate the effects of an established cell sheet manipulation technique, cell sheet multilayering, on fabrication of MSC-derived hyaline-like cartilage 3D layered constructs in vitro. To achieve this goal, multilayered MSC sheets are prepared and assessed for structural and biochemical transitions throughout chondrogenesis. Results support MSC multilayering as a means of increasing construct thickness and 3D cellular interactions related to in vitro chondrogenesis, including N-cadherin, connexin 43, and integrin β-1. Data indicate that increasing construct thickness from 14 μm (1-layer construct) to 25 μm (2-layer construct) increases these cellular interactions and subsequent in vitro MSC chondrogenesis. However, a clear initial thickness threshold (33 μm - 3-layer construct) is evident that decreases the rate and extent of in vitro chondrogenesis, specifically chondrogenic gene expressions (Sox9, aggrecan, type II collagen) and sulfated proteoglycan accumulation in deposited extracellular matrix (ECM). Together, these data support the utility of cell sheet multilayering as a platform for tailoring construct thickness and subsequent MSC chondrogenesis for future articular cartilage regeneration applications.
format	article
author	Hallie Thorp Kyungsook Kim Sophia Bou-Ghannam Makoto Kondo Travis Maak David W. Grainger Teruo Okano
author_facet	Hallie Thorp Kyungsook Kim Sophia Bou-Ghannam Makoto Kondo Travis Maak David W. Grainger Teruo Okano
author_sort	Hallie Thorp
title	Enhancing chondrogenic potential via mesenchymal stem cell sheet multilayering
title_short	Enhancing chondrogenic potential via mesenchymal stem cell sheet multilayering
title_full	Enhancing chondrogenic potential via mesenchymal stem cell sheet multilayering
title_fullStr	Enhancing chondrogenic potential via mesenchymal stem cell sheet multilayering
title_full_unstemmed	Enhancing chondrogenic potential via mesenchymal stem cell sheet multilayering
title_sort	enhancing chondrogenic potential via mesenchymal stem cell sheet multilayering
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e3e2a85b4c104a94a2a41d7f88aab775
work_keys_str_mv	AT halliethorp enhancingchondrogenicpotentialviamesenchymalstemcellsheetmultilayering AT kyungsookkim enhancingchondrogenicpotentialviamesenchymalstemcellsheetmultilayering AT sophiaboughannam enhancingchondrogenicpotentialviamesenchymalstemcellsheetmultilayering AT makotokondo enhancingchondrogenicpotentialviamesenchymalstemcellsheetmultilayering AT travismaak enhancingchondrogenicpotentialviamesenchymalstemcellsheetmultilayering AT davidwgrainger enhancingchondrogenicpotentialviamesenchymalstemcellsheetmultilayering AT teruookano enhancingchondrogenicpotentialviamesenchymalstemcellsheetmultilayering
_version_	1718373001562423296

Enhancing chondrogenic potential via mesenchymal stem cell sheet multilayering

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