Characterization of Juvenile Hormone Related Genes Regulating Cantharidin Biosynthesis in Epicauta chinensis

Abstract Cantharidin is a defensive toxin biosynthesized by blister beetles. It is well known for medical applications and toxicity. However, the biosynthesis process of cantharidin is still not well understood. In the present study, three genes (methyl farnesoate epoxidase (EcMFE), juvenile hormone...

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Autores principales:	Ming Jiang, Shumin Lü, Yalin Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e3e42c6c624c40a78a5c61db59c009fc
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spelling	oai:doaj.org-article:e3e42c6c624c40a78a5c61db59c009fc2021-12-02T15:05:23ZCharacterization of Juvenile Hormone Related Genes Regulating Cantharidin Biosynthesis in Epicauta chinensis10.1038/s41598-017-02393-w2045-2322https://doaj.org/article/e3e42c6c624c40a78a5c61db59c009fc2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02393-whttps://doaj.org/toc/2045-2322Abstract Cantharidin is a defensive toxin biosynthesized by blister beetles. It is well known for medical applications and toxicity. However, the biosynthesis process of cantharidin is still not well understood. In the present study, three genes (methyl farnesoate epoxidase (EcMFE), juvenile hormone acid O-methyltransferase (EcJHAMT) and juvenile hormone epoxide hydrolase (EcJHEH)) were identified from Epicauta chinensis. The temporal and spatial expression patterns of these three genes revealed that the expression levels of EcMFE and EcJHEH were high in the first instar larval stage of E. chinensis with EcJHEH transcripts highest in the fifth larval instar. The expression level of EcJHAMT was significantly higher in the 2nd and 3rd larval instars. The transcripts of EcMFE, EcJHEH and EcJHAMT showed a similar tendency with the cantharidin production in male blister beetles after mating. We verified the functions of these three genes in cantharidin biosynthesis using the RNA interference method. Interference of EcMFE and EcJHEH significantly inhibited the biosynthesis of cantharidin in male E. chinensis after mating, but interference of EcJHAMT has no apparent influence on cantharidin biosynthesis. We propose that EcMFE and EcJHEH may be involved in the biosynthesis of cantharidin, but JH III might not be the direct precursor of cantharidin.Ming JiangShumin LüYalin ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ming Jiang Shumin Lü Yalin Zhang Characterization of Juvenile Hormone Related Genes Regulating Cantharidin Biosynthesis in Epicauta chinensis
description	Abstract Cantharidin is a defensive toxin biosynthesized by blister beetles. It is well known for medical applications and toxicity. However, the biosynthesis process of cantharidin is still not well understood. In the present study, three genes (methyl farnesoate epoxidase (EcMFE), juvenile hormone acid O-methyltransferase (EcJHAMT) and juvenile hormone epoxide hydrolase (EcJHEH)) were identified from Epicauta chinensis. The temporal and spatial expression patterns of these three genes revealed that the expression levels of EcMFE and EcJHEH were high in the first instar larval stage of E. chinensis with EcJHEH transcripts highest in the fifth larval instar. The expression level of EcJHAMT was significantly higher in the 2nd and 3rd larval instars. The transcripts of EcMFE, EcJHEH and EcJHAMT showed a similar tendency with the cantharidin production in male blister beetles after mating. We verified the functions of these three genes in cantharidin biosynthesis using the RNA interference method. Interference of EcMFE and EcJHEH significantly inhibited the biosynthesis of cantharidin in male E. chinensis after mating, but interference of EcJHAMT has no apparent influence on cantharidin biosynthesis. We propose that EcMFE and EcJHEH may be involved in the biosynthesis of cantharidin, but JH III might not be the direct precursor of cantharidin.
format	article
author	Ming Jiang Shumin Lü Yalin Zhang
author_facet	Ming Jiang Shumin Lü Yalin Zhang
author_sort	Ming Jiang
title	Characterization of Juvenile Hormone Related Genes Regulating Cantharidin Biosynthesis in Epicauta chinensis
title_short	Characterization of Juvenile Hormone Related Genes Regulating Cantharidin Biosynthesis in Epicauta chinensis
title_full	Characterization of Juvenile Hormone Related Genes Regulating Cantharidin Biosynthesis in Epicauta chinensis
title_fullStr	Characterization of Juvenile Hormone Related Genes Regulating Cantharidin Biosynthesis in Epicauta chinensis
title_full_unstemmed	Characterization of Juvenile Hormone Related Genes Regulating Cantharidin Biosynthesis in Epicauta chinensis
title_sort	characterization of juvenile hormone related genes regulating cantharidin biosynthesis in epicauta chinensis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e3e42c6c624c40a78a5c61db59c009fc
work_keys_str_mv	AT mingjiang characterizationofjuvenilehormonerelatedgenesregulatingcantharidinbiosynthesisinepicautachinensis AT shuminlu characterizationofjuvenilehormonerelatedgenesregulatingcantharidinbiosynthesisinepicautachinensis AT yalinzhang characterizationofjuvenilehormonerelatedgenesregulatingcantharidinbiosynthesisinepicautachinensis
_version_	1718388863060148224

Characterization of Juvenile Hormone Related Genes Regulating Cantharidin Biosynthesis in Epicauta chinensis

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