Use of Microsatellites in Genetic Diversity Assessment, Parentage Testing and Individual Identification of the Kangal Shepherd Dog

The Kangal Shepherd Dog is considered the most common dog breed of Turkish origin. This study investigated variations in ten autosomal microsatellite markers (PEZ01, PEZ03, PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 and FHC2079) for the purposes of genetic diversity assessment of the Kangal...

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Autores principales:	Vladimir Dimitrijević, Marko Ristanić, Ljubodrag Stanisić, Darko Drobnjak, Milivoje Urosević, Umit Ozkanal, Zoran Stanimirović
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2020
Materias:	allele frequency canine microsatellites genetic characterization kangal parentage testing Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/e3e4afe556b445aca0737bb5d1df696c
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spelling	oai:doaj.org-article:e3e4afe556b445aca0737bb5d1df696c2021-11-17T21:27:53ZUse of Microsatellites in Genetic Diversity Assessment, Parentage Testing and Individual Identification of the Kangal Shepherd Dog1820-744810.2478/acve-2020-0012https://doaj.org/article/e3e4afe556b445aca0737bb5d1df696c2020-06-01T00:00:00Zhttps://doi.org/10.2478/acve-2020-0012https://doaj.org/toc/1820-7448The Kangal Shepherd Dog is considered the most common dog breed of Turkish origin. This study investigated variations in ten autosomal microsatellite markers (PEZ01, PEZ03, PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 and FHC2079) for the purposes of genetic diversity assessment of the Kangal breed. In addition, the use of markers was assessed in parentage testing and individual identification within the Kangal breed. The microsatellite markers were typed in 51 Kangal dogs. The total number of alleles in the study population was 69. The mean number of alleles per locus was 6.9, and varied from four (FHC2079) to 12 (PEZ12). The polymorphic information content (PIC) ranged from 0.52 (FHC2079) to 0.87 (PEZ12), with the mean value for all loci of 0.717. Power of exclusion (PE) in 10 microsatellites investigated varied between 0.143 (FHC2054) and 0.472 (PEZ08) per locus. In order to determine the efficiency of using microsatellites for individual identification in the Kangal breed, power of discrimination (PD) and matching probability (MP) were calculated for each microsatellite marker. The panel achieved high combined MP (6.77 × 10-10) and high combined PD value of 99.99999%. The obtained results may contribute to further recognition of the Kangal breed, and confirm that the investigated microsatellites enable a reliable parentage testing and individual identification of the breed.Vladimir DimitrijevićMarko RistanićLjubodrag StanisićDarko DrobnjakMilivoje UrosevićUmit OzkanalZoran StanimirovićSciendoarticleallele frequencycanine microsatellitesgenetic characterizationkangalparentage testingVeterinary medicineSF600-1100ENActa Veterinaria, Vol 70, Iss 2, Pp 170-181 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	allele frequency canine microsatellites genetic characterization kangal parentage testing Veterinary medicine SF600-1100
spellingShingle	allele frequency canine microsatellites genetic characterization kangal parentage testing Veterinary medicine SF600-1100 Vladimir Dimitrijević Marko Ristanić Ljubodrag Stanisić Darko Drobnjak Milivoje Urosević Umit Ozkanal Zoran Stanimirović Use of Microsatellites in Genetic Diversity Assessment, Parentage Testing and Individual Identification of the Kangal Shepherd Dog
description	The Kangal Shepherd Dog is considered the most common dog breed of Turkish origin. This study investigated variations in ten autosomal microsatellite markers (PEZ01, PEZ03, PEZ05, PEZ06, PEZ08, PEZ12, PEZ20, FHC2010, FHC2054 and FHC2079) for the purposes of genetic diversity assessment of the Kangal breed. In addition, the use of markers was assessed in parentage testing and individual identification within the Kangal breed. The microsatellite markers were typed in 51 Kangal dogs. The total number of alleles in the study population was 69. The mean number of alleles per locus was 6.9, and varied from four (FHC2079) to 12 (PEZ12). The polymorphic information content (PIC) ranged from 0.52 (FHC2079) to 0.87 (PEZ12), with the mean value for all loci of 0.717. Power of exclusion (PE) in 10 microsatellites investigated varied between 0.143 (FHC2054) and 0.472 (PEZ08) per locus. In order to determine the efficiency of using microsatellites for individual identification in the Kangal breed, power of discrimination (PD) and matching probability (MP) were calculated for each microsatellite marker. The panel achieved high combined MP (6.77 × 10-10) and high combined PD value of 99.99999%. The obtained results may contribute to further recognition of the Kangal breed, and confirm that the investigated microsatellites enable a reliable parentage testing and individual identification of the breed.
format	article
author	Vladimir Dimitrijević Marko Ristanić Ljubodrag Stanisić Darko Drobnjak Milivoje Urosević Umit Ozkanal Zoran Stanimirović
author_facet	Vladimir Dimitrijević Marko Ristanić Ljubodrag Stanisić Darko Drobnjak Milivoje Urosević Umit Ozkanal Zoran Stanimirović
author_sort	Vladimir Dimitrijević
title	Use of Microsatellites in Genetic Diversity Assessment, Parentage Testing and Individual Identification of the Kangal Shepherd Dog
title_short	Use of Microsatellites in Genetic Diversity Assessment, Parentage Testing and Individual Identification of the Kangal Shepherd Dog
title_full	Use of Microsatellites in Genetic Diversity Assessment, Parentage Testing and Individual Identification of the Kangal Shepherd Dog
title_fullStr	Use of Microsatellites in Genetic Diversity Assessment, Parentage Testing and Individual Identification of the Kangal Shepherd Dog
title_full_unstemmed	Use of Microsatellites in Genetic Diversity Assessment, Parentage Testing and Individual Identification of the Kangal Shepherd Dog
title_sort	use of microsatellites in genetic diversity assessment, parentage testing and individual identification of the kangal shepherd dog
publisher	Sciendo
publishDate	2020
url	https://doaj.org/article/e3e4afe556b445aca0737bb5d1df696c
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Use of Microsatellites in Genetic Diversity Assessment, Parentage Testing and Individual Identification of the Kangal Shepherd Dog

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