In vitro fibrillogenesis of tropocollagen type III in collagen type I affects its relative fibrillar topology and mechanics

Abstract Tropocollagen types I and III were simultaneously fibrilized in vitro, and the differences between the geometric and mechanical properties of the heterotypic fibrils with different mixing ratios of tropocollagen III to I were investigated. Transmission electron microscopy was used to confir...

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Autores principales:	Meisam Asgari, Neda Latifi, Hossein K. Heris, Hojatollah Vali, Luc Mongeau
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e3e8131e5e4345a19217f20a5f425a92
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spelling	oai:doaj.org-article:e3e8131e5e4345a19217f20a5f425a922021-12-02T11:41:11ZIn vitro fibrillogenesis of tropocollagen type III in collagen type I affects its relative fibrillar topology and mechanics10.1038/s41598-017-01476-y2045-2322https://doaj.org/article/e3e8131e5e4345a19217f20a5f425a922017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01476-yhttps://doaj.org/toc/2045-2322Abstract Tropocollagen types I and III were simultaneously fibrilized in vitro, and the differences between the geometric and mechanical properties of the heterotypic fibrils with different mixing ratios of tropocollagen III to I were investigated. Transmission electron microscopy was used to confirm the simultaneous presence of both tropocollagen types within the heterotypic fibrils. The incorporation of collagen III in I caused the fibrils to be thinner with a shorter D-banding than pure collagen I. Hertzian contact model was used to obtain the elastic moduli from atomic force microscope indentation testing using a force volume analysis. The results indicated that an increase in the percentage of tropocollagen III reduced the mechanical stiffness of the obtained fibrils. The mechanical stiffness of the collagen fibrils was found to be greater at higher loading frequencies. This observation might explain the dominance of collagen III over I in soft distensible organs such as human vocal folds.Meisam AsgariNeda LatifiHossein K. HerisHojatollah ValiLuc MongeauNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meisam Asgari Neda Latifi Hossein K. Heris Hojatollah Vali Luc Mongeau In vitro fibrillogenesis of tropocollagen type III in collagen type I affects its relative fibrillar topology and mechanics
description	Abstract Tropocollagen types I and III were simultaneously fibrilized in vitro, and the differences between the geometric and mechanical properties of the heterotypic fibrils with different mixing ratios of tropocollagen III to I were investigated. Transmission electron microscopy was used to confirm the simultaneous presence of both tropocollagen types within the heterotypic fibrils. The incorporation of collagen III in I caused the fibrils to be thinner with a shorter D-banding than pure collagen I. Hertzian contact model was used to obtain the elastic moduli from atomic force microscope indentation testing using a force volume analysis. The results indicated that an increase in the percentage of tropocollagen III reduced the mechanical stiffness of the obtained fibrils. The mechanical stiffness of the collagen fibrils was found to be greater at higher loading frequencies. This observation might explain the dominance of collagen III over I in soft distensible organs such as human vocal folds.
format	article
author	Meisam Asgari Neda Latifi Hossein K. Heris Hojatollah Vali Luc Mongeau
author_facet	Meisam Asgari Neda Latifi Hossein K. Heris Hojatollah Vali Luc Mongeau
author_sort	Meisam Asgari
title	In vitro fibrillogenesis of tropocollagen type III in collagen type I affects its relative fibrillar topology and mechanics
title_short	In vitro fibrillogenesis of tropocollagen type III in collagen type I affects its relative fibrillar topology and mechanics
title_full	In vitro fibrillogenesis of tropocollagen type III in collagen type I affects its relative fibrillar topology and mechanics
title_fullStr	In vitro fibrillogenesis of tropocollagen type III in collagen type I affects its relative fibrillar topology and mechanics
title_full_unstemmed	In vitro fibrillogenesis of tropocollagen type III in collagen type I affects its relative fibrillar topology and mechanics
title_sort	in vitro fibrillogenesis of tropocollagen type iii in collagen type i affects its relative fibrillar topology and mechanics
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e3e8131e5e4345a19217f20a5f425a92
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In vitro fibrillogenesis of tropocollagen type III in collagen type I affects its relative fibrillar topology and mechanics

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